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SomeGuy
I feel better now :)


Registered: 04/18/10
Posts: 7,496
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Last seen: 9 years, 8 months
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pf tek c2c inoculation! updated 1
#12752577 - 06/16/10 01:28 PM (13 years, 7 months ago) |
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So, some people have been asking me about my super simple technique, so here it is.Cake-2-cake this is what you need. A fully colonized pf cake, a couple of syringes, some tp, napkins or whatever, and alcohol. here's what I do, fill the syringes under the tap. My water is treated, so I know it's sterile. flaming the needle. as soon as I move it from the flame, drape it with the alcohol soaked paper towel or whatever. next clean your lid with alcahol, insert the needle. As you can see in this pic, you want to turn the cake on it's side, and go in the bottom hole. If you don't do that, the cake will absorb all the water and you won't get any back! here's a closer look. You need to move the needle back and forth to tear up the mycelium, but try to stay on the outermost part of the cake, not all up in the substrate. Squirt the water. Look at the tip of the syringe. You need to see the hole in the end of the needle, so that you can keep it against the glass. This is so you can keep the bigger pieces of verm. out, they will clog the syringe. Go slow. Move the jar a little to manipulate the water back towards the syringe, try to get most of it. here's what I got back, 12 &15cc's that's more than enough for 10 cakes. That's it. Give the cake a couple days to heal before birthing. Proceed with inoculation in your normal manner. The best thing about this tek is that the mycelium moves forward genetically, instead of starting over with spores each time. Over time the myc. best suited to brf will predominate and your mush get bigger and better each time. first generation MS after 7 generations doing it this way. Questions and comments are welcome, lets work together, and this will really be great!
Edited by SomeGuy (06/27/10 02:20 PM)
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bootedboy
SpaceCowboy



Registered: 06/30/08
Posts: 1,381
Last seen: 2 months, 2 days
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Re: pf tek c2c inoculation! [Re: SomeGuy] 1
#12752674 - 06/16/10 01:51 PM (13 years, 7 months ago) |
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Quote:
paducahovoids said: but try to stay on the outermost part of the cake, not all up in the substrate.
I like to get all up in the substrate. 
Nice tek!!! May wanna spell check it, just because it is a tek. Alcahol is spelled alcohol.
-------------------- Ask me about ARX!!!
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SomeGuy
I feel better now :)


Registered: 04/18/10
Posts: 7,496
Loc:
Last seen: 9 years, 8 months
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Re: pf tek c2c inoculation! [Re: bootedboy]
#12752689 - 06/16/10 01:55 PM (13 years, 7 months ago) |
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duly noted!
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wygram
Myconaut

Registered: 01/28/07 
Posts: 573
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Re: pf tek c2c inoculation! [Re: SomeGuy]
#12753023 - 06/16/10 02:55 PM (13 years, 7 months ago) |
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Quote:
paducahovoids said: My water is treated, so I know it's sterile.
Unless it has so much chlorine that it would be dangerous to consume it is not sterile or close to it. Water treatment plants use giant sand filters that colonize with bacterial sludge to trap more bacteria. Water from the faucet is never going to be sterile.
To get some clean water: boil water for a 10-20 minutes and then suck it up while it's still boiling.
-------------------- Changing your mind is one of the best ways of finding out whether or not you still have one.
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Numinosum
President of Turd Town


Registered: 05/19/09
Posts: 1,175
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Re: pf tek c2c inoculation! [Re: wygram]
#12753159 - 06/16/10 03:22 PM (13 years, 7 months ago) |
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Quote:
wygram said:
Quote:
paducahovoids said: My water is treated, so I know it's sterile.
Unless it has so much chlorine that it would be dangerous to consume it is not sterile or close to it. Water treatment plants use giant sand filters that colonize with bacterial sludge to trap more bacteria. Water from the faucet is never going to be sterile.
To get some clean water: boil water for a 10-20 minutes and then suck it up while it's still boiling.
Yeah I agree with this, even if you have an inline germicidal UV the water still passes through the following pipes to get to the faucet, then through the screen etc. Every replace a dirty screen?
And your filling it in the clearly unsterile sink along with the clearly unsterile dishes.
-------------------- ...within my memory is the knowledge of hyper-light drive ships and how to build them. Doc_T's Efficiency Challenge
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SomeGuy
I feel better now :)


Registered: 04/18/10
Posts: 7,496
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Re: pf tek c2c inoculation! [Re: Numinosum]
#12753175 - 06/16/10 03:27 PM (13 years, 7 months ago) |
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true, but clearly sterile enough, thats the secret, working between the syringe and jar, keeps it sterile, and as far as the tap water, I've been using this technique for 2 years and never had a prob.
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GaneshNoIgnorance
remover of ignorance



Registered: 04/13/10
Posts: 691
Last seen: 2 months, 21 days
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Re: pf tek c2c inoculation! [Re: SomeGuy]
#12753309 - 06/16/10 03:51 PM (13 years, 7 months ago) |
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Do you have a dry verm layer?
-------------------- "I have come to tell you that you are free. Many ages ago, My consciousness left man, that he might develop himself. I return to find this development approaching completion, but hindered by fear and by misunderstanding. You have built for yourselves psychic suits of armor, and clad in them, your vision is restricted, your movements are clumsy and painful, your skin is bruised, and your spirit is broiled in the sun. I am chaos. I am the substance from which your artists and scientists build rhythms. I am the spirit with which your children and clowns laugh in happy anarchy. I am chaos. I am alive, and I tell you that you are free." HAIL ERIS! ALL HAIL DISCORDIA!
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anonjon
Partially Right

Registered: 11/03/08
Posts: 6,322
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I can't understand why you stick with cakes for 2 years. Have you tried growing grain spawn?
-------------------- The above post is fictional, hypothetical, or downright nonsensical.
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Shea25
Just some guy



Registered: 01/27/09
Posts: 7,772
Loc: Westcoast Canada
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Re: pf tek c2c inoculation! [Re: SomeGuy]
#12754064 - 06/16/10 06:06 PM (13 years, 7 months ago) |
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Mold spores can easily fall into the water as it leaves the faucet, if this a tek for other people you should recommend sterilizing the syringe and water.
Use a tek like this for getting a sterilize syringe http://www.shroomery.org/forums/showflat.php/Number/12098357#12098357
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SomeGuy
I feel better now :)


Registered: 04/18/10
Posts: 7,496
Loc:
Last seen: 9 years, 8 months
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Re: pf tek c2c inoculation! [Re: Shea25]
#12754376 - 06/16/10 07:05 PM (13 years, 7 months ago) |
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1 I do have a dry verm. layer on that. It is a standard pf 2 I've done grain, but did you see how dirty my house is? This was a super easy simple way to continue the strain, plus it's about 10 days faster +-than waiting for spores and a couple less steps 3 Mold spores cold never Easily fall in the middle oF a vigourously running faucet, it's a liquid flow hood
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Base Icks



Registered: 03/19/10
Posts: 6,191
Loc: Shroomshire
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Re: pf tek c2c inoculation! [Re: SomeGuy]
#12754807 - 06/16/10 08:28 PM (13 years, 7 months ago) |
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Quote:
paducahovoids said: it's a liquid flow hood
Made me lol
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Sanguine
Candy flippin'



Registered: 11/08/09
Posts: 1,332
Loc: Southeast
Last seen: 6 years, 16 days
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Re: pf tek c2c inoculation! [Re: Base Icks]
#12754833 - 06/16/10 08:33 PM (13 years, 7 months ago) |
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This is sweet!
--------------------
   "The greatest delight which the fields and woods minister is the suggestion of an occult relation between man and the vegetable. I am not alone and unacknowledged. They nod to me, and I to them." --Ralph Waldo Emerson Ozz's Poo Pictorial Shirley Knott's Spore Print Tek No PC wbs APE grow log
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Doc_T
Random Dude




Registered: 03/06/09
Posts: 42,395
Loc: Colorado
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Re: pf tek c2c inoculation! [Re: anonjon]
#12754864 - 06/16/10 08:39 PM (13 years, 7 months ago) |
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Quote:
anonjon said: I can't understand why you stick with cakes for 2 years.
Because cakes rawk hard. Have you tried cakes? 
-------------------- You make it all possible. Doesn't it feel good?
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bootedboy
SpaceCowboy



Registered: 06/30/08
Posts: 1,381
Last seen: 2 months, 2 days
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Re: pf tek c2c inoculation! [Re: Doc_T]
#12755189 - 06/16/10 09:36 PM (13 years, 7 months ago) |
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Quote:
Doc_T said:
Quote:
anonjon said: I can't understand why you stick with cakes for 2 years.
Because cakes rawk hard. Have you tried cakes? 
Dude Anonjon is a TC Doc and his PE straw basket grow rocks.  He isn't gonna waste any time with cakes, but I still do on ocassion.
-------------------- Ask me about ARX!!!
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Doc_T
Random Dude




Registered: 03/06/09
Posts: 42,395
Loc: Colorado
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Re: pf tek c2c inoculation! [Re: bootedboy]
#12755206 - 06/16/10 09:39 PM (13 years, 7 months ago) |
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Quote:
bootedboy said: Dude Anonjon is a TC Doc .

I hadn't noticed.

It's ok to disagree with the TC's. They're just people.
-------------------- You make it all possible. Doesn't it feel good?
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Shea25
Just some guy



Registered: 01/27/09
Posts: 7,772
Loc: Westcoast Canada
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Re: pf tek c2c inoculation! [Re: Doc_T]
#12755229 - 06/16/10 09:43 PM (13 years, 7 months ago) |
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Quote:
Doc_T said:
Quote:
bootedboy said: Dude Anonjon is a TC Doc .

I hadn't noticed.

It's ok to disagree with the TC's. They're just people.
Its true there are plenty of people on here with just as much knowledge.
Cakes do rock
Blam, especially when you can barely see the cake

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SomeGuy
I feel better now :)


Registered: 04/18/10
Posts: 7,496
Loc:
Last seen: 9 years, 8 months
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Re: pf tek c2c inoculation! [Re: Shea25]
#12755566 - 06/16/10 10:40 PM (13 years, 7 months ago) |
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sure grains rock...bulk rocks....poo rocks, but I get an honest 12-15(up to 18)g off of 4 flushes per pint in 28 days. It doesn't take many cakes to have more than I need, and it works. I could try a grain to coir or something, but it's been done. I haven't seen this technique demonstrated. You could knock up a lc or bulk with it too
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Shea25
Just some guy



Registered: 01/27/09
Posts: 7,772
Loc: Westcoast Canada
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Re: pf tek c2c inoculation! [Re: SomeGuy]
#12755581 - 06/16/10 10:42 PM (13 years, 7 months ago) |
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Quote:
12-15(up to 18)g off of 4 flushes per pint
Use half pints they preform better then pints.
I can get 13+ dry grams from a 1/2 pint cake 3 flushes
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bootedboy
SpaceCowboy



Registered: 06/30/08
Posts: 1,381
Last seen: 2 months, 2 days
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Re: pf tek c2c inoculation! [Re: Shea25]
#12755626 - 06/16/10 10:47 PM (13 years, 7 months ago) |
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Quote:
Shea25 said: Its true there are plenty of people on here with just as much knowledge
I agree, and you are one of them Shea25, but it is also reasonable to assume that a TC knows what they are talking about and their grows are of a certain caliber so it is not a big surprise when they dis on cakes.
-------------------- Ask me about ARX!!!
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SomeGuy
I feel better now :)


Registered: 04/18/10
Posts: 7,496
Loc:
Last seen: 9 years, 8 months
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Re: pf tek c2c inoculation! [Re: Shea25]
#12755637 - 06/16/10 10:50 PM (13 years, 7 months ago) |
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apparently I need to use your substrate, what is it, alien?
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