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Invisibledeprave
GrandmasterToker

Registered: 11/12/02
Posts: 366
Loc: DownUnder
My setup *DELETED*
    #1269514 - 02/01/03 10:20 PM (14 years, 6 months ago)

Post deleted by deprave


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Invisibledeprave
GrandmasterToker

Registered: 11/12/02
Posts: 366
Loc: DownUnder
Re: My setup [Re: deprave]
    #1269520 - 02/01/03 10:21 PM (14 years, 6 months ago)

omg i fergot so much shit lol one sec


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Invisibledeprave
GrandmasterToker

Registered: 11/12/02
Posts: 366
Loc: DownUnder
Re: My setup [Re: deprave]
    #1269530 - 02/01/03 10:25 PM (14 years, 6 months ago)

ok the INCOLATION:

The jars are allowed to cool for 24 hours, they are then removed from the pc after the kitchen has been disinfacted, the oven is set to 200 jars are placed on the extended rack, make sure airbuble is in syringe, flame sterlize with bic till its red hot, wait 5 minutes or so, tinfoil is removed, syringe has been shooked througly during waiting for cooling of needle, each jar is shot up with 1cc on each hole in which i watch the spore water go into the jar and flow on the substrate, they are quickly taped up, also besided the oven tek i have tried using my home-made glovebox, im almost convinced this isnt the time of contamination


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Anonymous

Re: My setup [Re: deprave]
    #1269535 - 02/01/03 10:27 PM (14 years, 6 months ago)

Temp the jars are setting at? Incubator? Light conditions?


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Invisibledeprave
GrandmasterToker

Registered: 11/12/02
Posts: 366
Loc: DownUnder
Re: My setup [Re: deprave]
    #1269543 - 02/01/03 10:29 PM (14 years, 6 months ago)

I made the syringes with prints, to do this I boiled the syringe a razor, a glass, rubber gloves, i then put the boiled water in the glass which was also boil, but plastic wrap ontop and let it cool in a sterile place, one everything had cooled i did the oven take to scrape the spoores into the glass of water with the sterilized blade, shook the glass up real good back and forth and sucked it up with the needle to make a long story short


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Invisibledeprave
GrandmasterToker

Registered: 11/12/02
Posts: 366
Loc: DownUnder
Re: My setup [Re: deprave]
    #1269546 - 02/01/03 10:30 PM (14 years, 6 months ago)

the jars a setting in an incubator that read around 80.2, its a very large rubbermaid box in a dark place


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Invisibledeprave
GrandmasterToker

Registered: 11/12/02
Posts: 366
Loc: DownUnder
Re: My setup [Re: ]
    #1269552 - 02/01/03 10:32 PM (14 years, 6 months ago)

Quote:

Jars are incubated at 82 F 40% humidity




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Invisibledeprave
GrandmasterToker

Registered: 11/12/02
Posts: 366
Loc: DownUnder
Re: My setup [Re: deprave]
    #1269555 - 02/01/03 10:33 PM (14 years, 6 months ago)

Ive been thru two equadorian prints and am now on Amazonian


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Anonymous

Re: My setup [Re: deprave]
    #1269563 - 02/01/03 10:36 PM (14 years, 6 months ago)

Perhaps it's not you, but your source? I haven't fruited anything, but I have never contamed a jar. I'll read the process again but I think it's safe to say you'r problem is the prints. I won't try a print till I fruit something and have the chance to make my own. Then again I'm a dog and don't know too much :smile: 


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Invisibledeprave
GrandmasterToker

Registered: 11/12/02
Posts: 366
Loc: DownUnder
Re: My setup [Re: ]
    #1269574 - 02/01/03 10:41 PM (14 years, 6 months ago)

Yea alright maybe it was i got one of the equadorians from some no name site on the web and i didnt know what the hell i was doing then, then i got on from a person that was new to printing and the whole shing also, but the new amazonians i got from MushiMUsh and i did get some dark sporewater from this one, also it still could take a bit longer for them to germ, i just want this time to be a winner, specialy cause my bday is coming up, any suggestiongs or anything woudl sure be help, i plan on doing cakes for the first flush and then casing with a 50/50 mix


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Anonymous

Re: My setup [Re: deprave]
    #1269593 - 02/01/03 10:52 PM (14 years, 6 months ago)

Hey Dawg, im still new so I can't say that much. But I think first things first, and if you'r starting from a print you have 2x the chance of fucking it up, as compared with starting with a syringe. Ya know? I figured I would start with the least amount of possiblities that are against me :wink: Don't let it get you down, 3 fruiting attempts here.....all to no avail :frown: I can grow jars like a mofo though :smile: 


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Invisibledeprave
GrandmasterToker

Registered: 11/12/02
Posts: 366
Loc: DownUnder
Re: My setup [Re: ]
    #1269602 - 02/01/03 10:55 PM (14 years, 6 months ago)

yea i tried with a straight syringe but my homemade ones are much darker and sexier


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InvisibleSubGen1us
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Registered: 11/26/02
Posts: 3,427
Re: My setup [Re: deprave]
    #1269743 - 02/02/03 12:22 AM (14 years, 6 months ago)

hello
Now that u have a pc why not do grains. I hear that people should start with the pf tek and i did the same. Problem is i could never get a cake to not contam.
Just lately i have gotten a pc like u. I decided to do some wild bird seed.
so far i am happy because i have a few jars of pesa right now that i innoced on the 25 and 8 days later(today) they are 80% - wat the heck i was thinking, why didnt i do this before.


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InvisibleSubGen1us
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Registered: 11/26/02
Posts: 3,427
Re: My setup [Re: SubGen1us]
    #1269747 - 02/02/03 12:25 AM (14 years, 6 months ago)

dont wait so long after steralizeing ur needle. heat it, whipe down with alcohol let dry for 5-10 seconds then innoculate.

also u could try putting the weight up tp 15 pounds


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Invisibledeprave
GrandmasterToker

Registered: 11/12/02
Posts: 366
Loc: DownUnder
Re: My setup [Re: SubGen1us]
    #1269759 - 02/02/03 12:34 AM (14 years, 6 months ago)

its a weight, its always 10 psi yea ive been thinking about birdseed, cant seem to find any millet without sunflower seeds except unshelled, but i herd thats good to no? My pc only holds half pints thou also, not sure bout birdseed in halfpints


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Invisibledeprave
GrandmasterToker

Registered: 11/12/02
Posts: 366
Loc: DownUnder
Re: My setup [Re: deprave]
    #1269760 - 02/02/03 12:35 AM (14 years, 6 months ago)

is amazonian a slow colonizer anyone know?


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