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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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Freespores.com
    #12691576 -

i think may of sent me a syringe that had some dormant contam within it, this is very possible correct?


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A man once said to a dervish "Why do I not see you more often?" The dervish replied "Because the words 'why have you not seen me more often?' are sweeter to my ear than the words 'why have you come again?'"

-Mulla Jami, Idries Shah's caravan of dreams, 1968

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Re: Freespores.com [Re: robbyr727]
    #12691603 -

Need more info than that. When did you notice contam and what methods did you use during inoculation? How did you sterilize jars?


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Ask me about ARX!!!

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Re: Freespores.com [Re: bootedboy]
    #12693444 -

Ok i guess i would say it started when i first got the spores in the mail, i eagerly wanted to attempt a LC so i did about 6 jars with the karo light corn syrup using about .7 - 1 cc per jar, 2 table spoons of karo per 200 ml of water, the water was pre boiled before going into the jars, then the jars where microwaved, the microwave was cleaned and generously rubbed down with 70% isopropyl alcohol, afterwards they were covered with tin foil, and the lids where fit over them rubberside up, not tightly but enough to where i could swirl the liquid around and tell if it was hot and not have it leak out on my hand, ANY WHO, after inoculation(yes i flame sterilized and cleaned with alcohol between each jar)  and about a week and a half of time there was a nice fluffy cloud  of white strands within two of the jars, it appeared to me as mycellium from the pictures i had witnessed on this website, only two of these  seemed legitimate the others had looked quite funky, i finally decide to test them in a brf verm jar, i made nine at first, dumb me, within two days these were contaminated with a hard green right at the inoculation point, ok bam those all go into the garbage, a few days later i think maybe i should just stick to spores directly into the substrate insted of doing the whole LC/agar kind of thing and keeping my cube strain alive for months and months, atleast untill i was more experience, so one night i sat down, made 3 jars brf style, did the same thing i alwas do, put them over a pan of boiling water on a steamer pan that fits snug right into the top of the other pan and a lid is place on top, then the water is boiled and the jars are steamed for 90 minutes then are cooled for 3-5 hours, the spores were injected into the cake, and guess what same thing, within two days a nasty green appears in the same exact place the cakes were inoculated.


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A man once said to a dervish "Why do I not see you more often?" The dervish replied "Because the words 'why have you not seen me more often?' are sweeter to my ear than the words 'why have you come again?'"

-Mulla Jami, Idries Shah's caravan of dreams, 1968

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Re: Freespores.com [Re: robbyr727]
    #12696071 -

Microwave sterilization is not a valid method of sterilization . It works for some but it's in the "use at your own peril" category.

Edit: also were the jars covered with anything when you microwaved them because if not when you took them out they were exposed to open contaminant laden air, so even if they had been successfully sterilized they would have been contaminated at that point.


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Wildflower seed on the sand and stone, may the four winds blow you safely home
Curriculum vapidum (dry herb vapes)

Edited by NizzyJones (06/06/10 09:22 AM)

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Re: Freespores.com [Re: NizzyJones]
    #12696517 -

Did you use a glovebox to do the inoculation work?


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Ask me about ARX!!!

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Re: Freespores.com [Re: robbyr727]
    #12739939 -

robbyr727 said:
cooled for 3-5 hours, the spores were injected into the cake, and guess what same thing, within two days a nasty green appears in the same exact place the cakes were inoculated.


The glovebox might be your solution, or did you consider that 3-5 hours is not adequate time to cool the jars? If the spores died on impact then the contaminants have nothing to compete with, and will grow freely. Most let their jars sit at least 12 hours, aka overnight, it seems.

Or, it could be the syringe.


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and let it be so Praise Psilocybe

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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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