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Amazon Shop: Agar, Petri Dish

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Invisibledog
straw dog

Registered: 06/20/02
Posts: 2,790
Loc: Route 66
dish sterilization
    #1265485 - 01/31/03 12:13 PM (18 years, 1 month ago)

I'm eager to continue my experimentation with agar but the only petri dishes I have on hand are some plastic disposable ones, and they have been used. Is there any reason why I should not use bleach to sterilize these dishes for re-use?

They melt in the PC.  :frown:

 


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Fascism (fash'izem) n. A governmental system marked by a centralized dictatorship, stringent socioeconomic controls, and often belligerent nationalism. see also: the Bush Administration.


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Offlinevatoloco
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Registered: 01/30/02
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Post deleted by MOE THE MAD SCIENTIST [Re: dog]
    #1265657 - 01/31/03 01:10 PM (18 years, 1 month ago)



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Invisibledog
straw dog

Registered: 06/20/02
Posts: 2,790
Loc: Route 66
Re: dish sterilization [Re: vatoloco]
    #1265706 - 01/31/03 01:25 PM (18 years, 1 month ago)

Positive. I wrapped two of them in aluminum foil and PC'd for 50 minutes at 15 PSI. When I unwrapped them afterwards I found shriveled blobs of plastic.


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Fascism (fash'izem) n. A governmental system marked by a centralized dictatorship, stringent socioeconomic controls, and often belligerent nationalism. see also: the Bush Administration.


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Offlinedebianlinux
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Re: dish sterilization [Re: dog]
    #1265766 - 01/31/03 01:54 PM (18 years, 1 month ago)

Quote:

I'm eager to continue my experimentation with agar but the only petri dishes I have on hand are some plastic disposable ones, and they have been used. Is there any reason why I should not use bleach to sterilize these dishes for re-use?

They melt in the PC.  :frown:

   




bleach is kinda "sticky". I've been told the best way to rinse bleach is to use alternating hot/cold running water (seems to work for me). because of the "stickiness" factor I'd recommend that you get a dish and fill it with alcohol and just soak them in there (that is what I do, works well), the alcohol evaporates just fine.


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Invisibledog
straw dog

Registered: 06/20/02
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Re: dish sterilization [Re: debianlinux]
    #1265807 - 01/31/03 02:05 PM (18 years, 1 month ago)

Good suggestion... how long do you soak?


--------------------


Fascism (fash'izem) n. A governmental system marked by a centralized dictatorship, stringent socioeconomic controls, and often belligerent nationalism. see also: the Bush Administration.


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Offlinedebianlinux
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Re: dish sterilization [Re: dog]
    #1265812 - 01/31/03 02:09 PM (18 years, 1 month ago)

Quote:

Good suggestion... how long do you soak? 




that really depends,... greatly. sometimes as long as 3 weeks :blush:. i'd say anywhere from 36-48 hours would be sufficient (1 hour is probably good but better safe than sorry, right?)


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Anonymous

Re: dish sterilization [Re: debianlinux]
    #1265832 - 01/31/03 02:20 PM (18 years, 1 month ago)

Quote:

Quote:

I'm eager to continue my experimentation with agar but the only petri dishes I have on hand are some plastic disposable ones, and they have been used. Is there any reason why I should not use bleach to sterilize these dishes for re-use?

They melt in the PC.  :frown:

   




bleach is kinda "sticky". I've been told the best way to rinse bleach is to use alternating hot/cold running water (seems to work for me). because of the "stickiness" factor I'd recommend that you get a dish and fill it with alcohol and just soak them in there (that is what I do, works well), the alcohol evaporates just fine. 



After washing with bleach use a dilute water and vinigar rinse. This cutes through the bleach film that was left after sterelization.


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Invisibledeanofmean
mycophagous

Registered: 12/07/02
Posts: 2,017
Loc: PNW
Re: dish sterilization [Re: dog]
    #1265859 - 01/31/03 02:34 PM (18 years, 1 month ago)

sorry if this sounds too obvious, but have you tried just boiling or the microwave ?
another thought, you could wipe bleach residue out with an isopropyl tissue in the glove .


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Amazon Shop: Agar, Petri Dish

Mushrooms, Mycology and Psychedelics >> Mushroom Cultivation

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