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spaceman101
Friend to all



Registered: 01/18/13
Posts: 11,726
Loc: In heaven bored as hell
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I don't think I've heard much at all of lophs being sprouted on agar but that shouldn't stop the fairy
Keep us updated on your germination rates on agar but be careful of the tap root while replanting the sprouts to good clean soil
-------------------- ------------- Check out my Pollen Trade thread for spreading Good genetics far and wide Great Vendors thread where we can discuss "Non Shroomery" Vendors that sell good products worth checking into A few things I wanna get my hands on check it out and let me know if you have any of these Need help getting started growing mushrooms Here's The Noob Forum
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mentalexploration
Salvia Cultivator


Registered: 11/01/15
Posts: 126
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Thank you for the advice. I'll post up some photos, when I get them going tomorrow.
-------------------- Have Blosser salvia plants and leaves available PM for trade!
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spaceman101
Friend to all



Registered: 01/18/13
Posts: 11,726
Loc: In heaven bored as hell
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Quote:
mentalexploration said: Thank you for the advice. I'll post up some photos, when I get them going tomorrow.

That's me btw
-------------------- ------------- Check out my Pollen Trade thread for spreading Good genetics far and wide Great Vendors thread where we can discuss "Non Shroomery" Vendors that sell good products worth checking into A few things I wanna get my hands on check it out and let me know if you have any of these Need help getting started growing mushrooms Here's The Noob Forum
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Raven44
Entry not permitted to muggles



Registered: 12/07/13
Posts: 1,970
Loc: My sovereign reality bubble
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Quote:
mentalexploration said: I'll be pouring agar in a petri dish, and praying to the fairy, as I sprinkle them onto the surface.
U gonna try and do some grafting to agar?
I plan on this in time..
U could make many many loph callus theoretically from one slice of a button...
From each callus, many lophs can be made basically
Maybe u can even go from loph button slice to rooting media. And be able to root many clones from one button slice???
Anyone know the best auxins and cytokins to use for cacti?
Hmmmm cacti biotechnology I'm thinkin....
Edited by Raven44 (03/05/16 11:05 PM)
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spaceman101
Friend to all



Registered: 01/18/13
Posts: 11,726
Loc: In heaven bored as hell
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Re: Loph Growers Unite! [Re: Raven44]
#22977970 - 03/05/16 11:12 PM (7 years, 10 months ago) |
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Lophs don't do so well being calloused and placed on a wet media so I would suspect failure from this endeavor but I'm still excited to see what you might be able to do
-------------------- ------------- Check out my Pollen Trade thread for spreading Good genetics far and wide Great Vendors thread where we can discuss "Non Shroomery" Vendors that sell good products worth checking into A few things I wanna get my hands on check it out and let me know if you have any of these Need help getting started growing mushrooms Here's The Noob Forum
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ferrel_human
stone eater



Registered: 06/26/09
Posts: 16,318
Loc: Texas
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Re: Loph Growers Unite! [Re: Raven44]
#22977990 - 03/05/16 11:16 PM (7 years, 10 months ago) |
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Man i wish i understood all these twchnical terms. Exactly what will ypu be doing with said seeds mentalexploration?
-------------------- Nature is my church and walking through it is gospel. It tells no lies and reveals all to those who look, and listen, closely. -Karode
 Looking for Mimosa tenuiflora seeds. Buttons for trade
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mentalexploration
Salvia Cultivator


Registered: 11/01/15
Posts: 126
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Re: Loph Growers Unite! [Re: Raven44]
#22978069 - 03/05/16 11:40 PM (7 years, 10 months ago) |
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Quote:
Raven44 said:
Quote:
mentalexploration said: I'll be pouring agar in a petri dish, and praying to the fairy, as I sprinkle them onto the surface.
U gonna try and do some grafting to agar?
I plan on this in time..
U could make many many loph callus theoretically from one slice of a button...
From each callus, many lophs can be made basically
Maybe u can even go from loph button slice to rooting media. And be able to root many clones from one button slice???
Anyone know the best auxins and cytokins to use for cacti?
Hmmmm cacti biotechnology I'm thinkin....
I'll have to first germinate seed onto agar, as I don't currently have any explant material to use, for inducing a callus. So I'll germinate seeds first, then once big enough, take some tissue and apply to an experimental callus medium. Once the calli have been induced, then I'll subculture and put onto a multiplication medium to grow it into an actual cacti, then off to rooting =)
-------------------- Have Blosser salvia plants and leaves available PM for trade!
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Raven44
Entry not permitted to muggles



Registered: 12/07/13
Posts: 1,970
Loc: My sovereign reality bubble
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Well maybe they would have to be simple placed onto rooting media and skip callus step...??
I haven't looked into it at all yet
If u could root little baby cuttings it'd still be sweet tho. Just not as sweet as forming callus, cause forming callus u can make more clones basically..
How do u know they won't like to form callus? Maybe u can use a high agar content, and a Petri dish with ventilation or something of the sort. Petri with gas exchange to keep humidty down is more like it.. filtered GE of course... just a thought. .. I see ur point totally i think
Cacti form callus in dry conditions I would think.. but maybe w the right media additives that wouldn't be the case??
After all plants can grow in the dark w out the need for light w the right media additives. And callus cells are special embryonic cells that have the ability to form any plant or in this case cacti organ...
I'm not saying ur wrong by any means, I haven't looked into this at all and need to. Maybe u have more so..??
Either way I will let u guys know when the time comes
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ferrel_human
stone eater



Registered: 06/26/09
Posts: 16,318
Loc: Texas
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Re: Loph Growers Unite! [Re: Raven44] 1
#22978101 - 03/05/16 11:52 PM (7 years, 10 months ago) |
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Quote:
mentalexploration said:
Quote:
Raven44 said:
Quote:
mentalexploration said: I'll be pouring agar in a petri dish, and praying to the fairy, as I sprinkle them onto the surface.
U gonna try and do some grafting to agar?
I plan on this in time..
U could make many many loph callus theoretically from one slice of a button...
From each callus, many lophs can be made basically
Maybe u can even go from loph button slice to rooting media. And be able to root many clones from one button slice???
Anyone know the best auxins and cytokins to use for cacti?
Hmmmm cacti biotechnology I'm thinkin....
I'll have to first germinate seed onto agar, as I don't currently have any explant material to use, for inducing a callus. So I'll germinate seeds first, then once big enough, take some tissue and apply to an experimental callus medium. Once the calli have been induced, then I'll subculture and put onto a multiplication medium to grow it into an actual cacti, then off to rooting =)
What?
-------------------- Nature is my church and walking through it is gospel. It tells no lies and reveals all to those who look, and listen, closely. -Karode
 Looking for Mimosa tenuiflora seeds. Buttons for trade
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Raven44
Entry not permitted to muggles



Registered: 12/07/13
Posts: 1,970
Loc: My sovereign reality bubble
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Quote:
mentalexploration said:
Quote:
Raven44 said:
Quote:
mentalexploration said: I'll be pouring agar in a petri dish, and praying to the fairy, as I sprinkle them onto the surface.
U gonna try and do some grafting to agar?
I plan on this in time..
U could make many many loph callus theoretically from one slice of a button...
From each callus, many lophs can be made basically
Maybe u can even go from loph button slice to rooting media. And be able to root many clones from one button slice???
Anyone know the best auxins and cytokins to use for cacti?
Hmmmm cacti biotechnology I'm thinkin....
I'll have to first germinate seed onto agar, as I don't currently have any explant material to use, for inducing a callus. So I'll germinate seeds first, then once big enough, take some tissue and apply to an experimental callus medium. Once the calli have been induced, then I'll subculture and put onto a multiplication medium to grow it into an actual cacti, then off to rooting =)
Right.. of course... can't do anything w seed except wait... lol
Once u have calli, it's onto a root initiation media... auxins are used here in combination w other things..
Then once u have roots, it's onto a shoot initiation media usually containing cytokins...
Gotta have roots before u can get a cacti shoots of u will when starting from calli I believe correct...?
Maybe u can go from calli to shoot initiation media, then to rooting media.. but then once it's rooted u will need to go to shoot media again... which prob won't matter much...
Otherwise u would have a cacti cut, which u immediately rooted. Then u can harden it off basically..
Anyways I wish u luck
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mentalexploration
Salvia Cultivator


Registered: 11/01/15
Posts: 126
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Re: Loph Growers Unite! [Re: Raven44]
#22978121 - 03/06/16 12:06 AM (7 years, 10 months ago) |
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Quote:
Raven44 said:
Quote:
mentalexploration said:
Quote:
Raven44 said:
Quote:
mentalexploration said: I'll be pouring agar in a petri dish, and praying to the fairy, as I sprinkle them onto the surface.
U gonna try and do some grafting to agar?
I plan on this in time..
U could make many many loph callus theoretically from one slice of a button...
From each callus, many lophs can be made basically
Maybe u can even go from loph button slice to rooting media. And be able to root many clones from one button slice???
Anyone know the best auxins and cytokins to use for cacti?
Hmmmm cacti biotechnology I'm thinkin....
I'll have to first germinate seed onto agar, as I don't currently have any explant material to use, for inducing a callus. So I'll germinate seeds first, then once big enough, take some tissue and apply to an experimental callus medium. Once the calli have been induced, then I'll subculture and put onto a multiplication medium to grow it into an actual cacti, then off to rooting =)
Right.. of course... can't do anything w seed except wait... lol
Once u have calli, it's onto a root initiation media... auxins are used here in combination w other things..
Then once u have roots, it's onto a shoot initiation media usually containing cytokins...
Gotta have roots before u can get a cacti shoots of u will when starting from calli I believe correct...?
Maybe u can go from calli to shoot initiation media, then to rooting media.. but then once it's rooted u will need to go to shoot media again... which prob won't matter much...
Otherwise u would have a cacti cut, which u immediately rooted. Then u can harden it off basically..
Anyways I wish u luck 
No need for roots at the moment.. Just need callus and then i can get them multiplied, and when so many are created, then they can be rooted. The purpose of agar media is to feed the nutrients to the plant/cacti, when roots are not present. After roots, it'll be going out of in vitro conditions.
-------------------- Have Blosser salvia plants and leaves available PM for trade!
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Raven44
Entry not permitted to muggles



Registered: 12/07/13
Posts: 1,970
Loc: My sovereign reality bubble
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I understand bro lol
Maybe Ur not understanding me entirely??
Obviously u don't need roots or whatever like u said....
I hate to start this on here... but must point out u seem to habe contradicted urself...
by saying: "then I'll subculture onto multiplication media and get some actual cacti, then off to rooting.."
And then saying: "no need for roots atm, just need callus and then can them multiplied, and when so many are created, then they can be rooted..:
Neways... good luck bro 
I'd be interested in ur experimental callus media, and rooting media, and shoot media I'll share mine when I research and mix it up...
Anyone wondering what we are talking about.. read this...
http://www.google.com/url?sa=t&source=web&cd=1&rct=j&q=biotechnology%20of%20cannabis%20sativa&ved=0ahUKEwiLxoHWxKvLAhWJtoMKHWkKCMYQFggfMAA&url=https%3A%2F%2Fwww.dmt-nexus.me%2Fusers%2Fcosmicspore%2FThe_Biotechnology_of_Cannabis_sativa_2nd_edition.pdf&usg=AFQjCNHhGHkwi0TF3ExAcHjqtQmY91w89A&sig2=qgu6B6qja7PPVvjl0BDI8w
We are simply discussing plant tissue culture methods, as rookies would lol. I will be using this for cannabis experiments first before cacti.
Edited by Raven44 (03/06/16 12:31 AM)
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ferrel_human
stone eater



Registered: 06/26/09
Posts: 16,318
Loc: Texas
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Re: Loph Growers Unite! [Re: Raven44]
#22978156 - 03/06/16 12:29 AM (7 years, 10 months ago) |
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Im gettong it now. What is included in agar? Whats it made of?
-------------------- Nature is my church and walking through it is gospel. It tells no lies and reveals all to those who look, and listen, closely. -Karode
 Looking for Mimosa tenuiflora seeds. Buttons for trade
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Raven44
Entry not permitted to muggles



Registered: 12/07/13
Posts: 1,970
Loc: My sovereign reality bubble
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Skip to tissue culture in the pdf...
U add vitamins and hormones ect... u need lab pipettes to dispense small . amounts of stuff..., .5 ul incriminates w some...
Orchids need totally duff stuff added than cannabis, cacti need totally diff stuff than bith of those I'm sure..
That pdf will steer u straight in no time.
Plant tissue culture is aka micro propagation
Edited by Raven44 (03/06/16 12:35 AM)
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mentalexploration
Salvia Cultivator


Registered: 11/01/15
Posts: 126
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Re: Loph Growers Unite! [Re: Raven44]
#22978261 - 03/06/16 02:41 AM (7 years, 10 months ago) |
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I guess you didn't understand me either. At the end of the day, they'll need roots for them to be given away to people, so they can be brought to nature, once they get big enough in the lab environment. Once they are out of being in vitro, roots are necessary, for survival. So, what I said was, no roots are needed for me to do callus, then to multiply as many as I would like, and let them get as big as I'd like, then, I can do roots, and place them outdoors in a good climate. The population of these things are decreasing. Re-introducing them is the goal of this experiment. Do you understand now?
-------------------- Have Blosser salvia plants and leaves available PM for trade!
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Raven44
Entry not permitted to muggles



Registered: 12/07/13
Posts: 1,970
Loc: My sovereign reality bubble
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I understand all that well friend, not sure where the confusion comes from...
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Raven44
Entry not permitted to muggles



Registered: 12/07/13
Posts: 1,970
Loc: My sovereign reality bubble
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Quote:
mentalexploration said:
Quote:
I'll have to first germinate seed onto agar, as I don't currently have any explant material to use, for inducing a callus. So I'll germinate seeds first, then once big enough, take some tissue and apply to an experimental callus medium. Once the calli have been induced, then I'll subculture and put onto a multiplication medium to grow it into an actual cacti, then off to rooting =)
I understand everything ur saying, u just didn't make sense to me here..
When u say," once calli are induced, then I'll subculture and put onto a multiplication media to grow it into an actual cacti, then off to rooting"...
If By " then subculture", u mean then u will place calli onto rooting media... that makes sense...
However u said u will be forming calli, then subculture and place onto multiplication media to grow into an actual cacti...
In my mind, the growing into an actual cacti part isn't until after u have roots formed.... this is my confusion
Yeah u gotta break up the calli, and each piece of each calli gets put onto multiplication media so that the calli can recover. In which case you then can place the recovered calli onto rooting media. Once roots formed, then onto shoot initiation media.
Then they can be hardened off and given to friends and planted in the wild ect..
Am I mistaken?
U can't form actual cacti (like u said/ i interpreted..) or actual "shoots if u will" or u can't put calli onto shoot initiation media until u have roots... this is what it seemed like u were saying
Edited by Raven44 (03/06/16 08:39 AM)
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Fuzz-nutter



Registered: 04/06/13
Posts: 1,740
Loc: Canada
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Re: Loph Growers Unite! [Re: Raven44]
#22978839 - 03/06/16 10:33 AM (7 years, 10 months ago) |
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First attempt at loph to trich (caespitosa) can't believe how fairly simple it was. It's now starting to show growth
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Bagels
Huntress



Registered: 07/11/14
Posts: 1,029
Loc: NZ
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That looks great! Thanks for sharing. Thinking about this myself
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FlyOnTheWall
Stranger


Registered: 06/14/15
Posts: 1,274
Last seen: 6 years, 19 days
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Quote:
Fuzz-nutter said:

First attempt at loph to trich (caespitosa) can't believe how fairly simple it was. It's now starting to show growth
That is good to hear. I am feeling tempted to try my hand at a loph to trich graft, but I have been a little intimidated, so I am happy to hear you say that it was easy.
I recently had to move into a new place and my growing situation is going to be less than ideal for the next 10 months.
I'm going to have to rely on artificial lighting during this time, so currently my lophs and 2 bridgesii are under 5 24w CFLs, and the trichs are of course already starting to look etiolated.
I read another post on here where intelligentlife was talking about using etiolated trichs as grafting stock for lophs.
He said etiolated trichs can make good grafting stock, and that then the trich/loph graft won't need as much light as a normal trich.
During these next 10 months, if I keep my trichs under these 5 24w CFL's, I know they will get really etiolated, so I am hoping that grafting a loph button onto the top of each trich might be a good solution to prevent this.
I really just want to keep my trichs alive for the next 10 months without them getting too badly deformed by etiolation and potentially becoming unhealthy. If I had lophs grafted on them I could degraft when I am in a new place and can keep them outside again.
Any thoughts on this from anyone? Does anyone think that might be a good idea?
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