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Offlineblackout
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new liquid innoc all look contamed will they work?
    #1260219 - 01/29/03 10:42 PM (21 years, 2 months ago)

i got some new malt extract for my liquid innocs or "honey water". i scraped some spores in and it made a sort of film in one day on the top of the liquid. i opened it and tossed it. it smelt fine. then i got a vendor syringe and put some in a fresh batch. got the same thing. when i shook it up it looked ok but there are no clouds of mycelium and it all forms a skim on the top. i have a similarly made jar with no spores in it and it has no growth at all so it is no sterilisation. i was very concious injecting the second jar.
has anybody had similar malt extract water that looked bad but actually worked?

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Offlinethe spiral
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Re: new liquid innoc all look contamed will they work? [Re: blackout]
    #1263047 - 01/30/03 06:20 PM (21 years, 2 months ago)

honey water can be a very very tricky thing. my friend has attempted it many times, but it's questionable if he's even gotten it to work once - it's very inviting to contams. That said, many people on here do report success with it - a lot of it is luck and your willingness to risk ruining a bunch of jars or bags.
After a couple days you should definately be seeing some cloudy or stringy growth in the solution. Don't shake the jar really hard, but swirl it to mix things up a bit.

Good luck!


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Re: new liquid innoc all look contamed will they work? [Re: the spiral]
    #1263452 - 01/30/03 08:36 PM (21 years, 2 months ago)

i think i will have to make some small jars with a small amount of substrate and try them before i go injecting my big bags

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Offlinedebianlinux
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Re: new liquid innoc all look contamed will they work? [Re: the spiral]
    #1264280 - 01/31/03 03:50 AM (21 years, 2 months ago)

Quote:

honey water can be a very very tricky thing. my friend has attempted it many times, but it's questionable if he's even gotten it to work once - it's very inviting to contams. That said, many people on here do report success with it - a lot of it is luck and your willingness to risk ruining a bunch of jars or bags.
After a couple days you should definately be seeing some cloudy or stringy growth in the solution. Don't shake the jar really hard, but swirl it to mix things up a bit.

Good luck!




i've never had anything but stellar success with honey water... seems like contams would make themselves rather apparent BEFORE you shot that crap in your jars... i dunno maybe I'm just stupendously lucky?? the trick, IMO, is to NEVER let the HW be exposed to air once it has been sterilized. that means making/using some sort of lid (see 6t's lid, or MM's) that allows entry of a needle without exposure to outside air. i have just used taped holes and made sure I was infront of a HEPA's outflow when I untaped and sucked up.

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Re: new liquid innoc all look contamed will they work? [Re: debianlinux]
    #1264336 - 01/31/03 04:19 AM (21 years, 2 months ago)

mine has a self healing lid on it so no air gets in at all. it smells fine but doesnt look it

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Offlinedebianlinux
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Re: new liquid innoc all look contamed will they work? [Re: blackout]
    #1264457 - 01/31/03 05:05 AM (21 years, 2 months ago)

Quote:

mine has a self healing lid on it so no air gets in at all. it smells fine but doesnt look it




you didn't outline your sterilization procedure or water/sugar ratio. optimal ratio is 96% water and 4% sugar. too much sugar and you will have problems. not sterilizing properly will obviously cause problems. i mix up the water/sugar and pc for 10-20 minutes at 10psi.

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