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Offlinetravis873o
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cease to show any progress after start....
    #12598841 - 05/20/10 03:21 PM (13 years, 8 months ago)

so i'm doing the PFtech and so far the mycelium is about in half dollar sized circles.  however, i have yet to see progress for about a week or 2 now.  i don't see any contamination. and they were kept at about 65 (out of my control, i know it's bad) but now they are at about 75-80, yet they still show no progress. Any thoughts of what the problem is and how to correct this?


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InvisibleDoc_T
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Re: cease to show any progress after start.... [Re: travis873o]
    #12598874 - 05/20/10 03:26 PM (13 years, 8 months ago)

pics?


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Offlinetravis873o
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Re: cease to show any progress after start.... [Re: Doc_T]
    #12598990 - 05/20/10 03:46 PM (13 years, 8 months ago)

the sharpie marks are just so i could check the progress and make sure i wasn't just imagining things.


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InvisibleSet
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Re: cease to show any progress after start.... [Re: travis873o]
    #12599045 - 05/20/10 03:53 PM (13 years, 8 months ago)

how large are your GE holes


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InvisibleDoc_T
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Re: cease to show any progress after start.... [Re: Set]
    #12599066 - 05/20/10 03:56 PM (13 years, 8 months ago)

Wrong jars, too compacted is my guess. And gas exchange maybe.


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You make it all possible. Doesn't it feel good?


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Offlinetravis873o
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Re: cease to show any progress after start.... [Re: Doc_T]
    #12599095 - 05/20/10 04:03 PM (13 years, 8 months ago)

if so, what kind of jars should i be using?

and the bottom ones, the ge holes are about an inch in diameter and the top is basically, the jar ring and the rest is a (can't remember the name for it) but a cotton disc that allows gas exchange. the 1 inch holes have this cotton disc as well


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Offlinetravis873o
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Re: cease to show any progress after start.... [Re: travis873o]
    #12603168 - 05/21/10 09:50 AM (13 years, 8 months ago)

bump......


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Offlinek00laid
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Re: cease to show any progress after start.... [Re: travis873o]
    #12603321 - 05/21/10 10:30 AM (13 years, 8 months ago)

hoping you mean tyvek. or polyfill (which isnt in discs)

also if thats the PF tek why didnt you use a dry verm layer as a contaminant barrier. it could either be lack of moisture, too compact (which i had troubles with as well) or lack of gas exchange. are you positive these cotton discs allow for decent air exchange?


what were your ratios for brf:water:verm.

because it seems you did not follow the pf tek closely


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Offlinetravis873o
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Re: cease to show any progress after start.... [Re: k00laid]
    #12606324 - 05/21/10 08:31 PM (13 years, 8 months ago)

there is a dry layer of verm ontop (about quarter to half inch ontop). and for ratios of verm and water, i just soaked the verm until it could hold no more water (there was water in the bottom of container)  and then added 1/4 cup of verm i believe, (i followed direction from a book). then added the brf from what the book called for.

and it's not tyvek it's synthetic filter discs http://www.mycosupply.com/cgi-bin/shopper.cgi?preadd=action&key=SFDI01

i didn't pack it into the jars either, i just spooned it in loosely


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InvisibleShea25
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Re: cease to show any progress after start.... [Re: travis873o]
    #12606336 - 05/21/10 08:33 PM (13 years, 8 months ago)

Quote:

travis873o said:
there is a dry layer of verm ontop (about quarter to half inch ontop). and for ratios of verm and water, i just soaked the verm until it could hold no more water (there was water in the bottom of container)  and then added 1/4 cup of verm i believe, (i followed direction from a book). then added the brf from what the book called for.

and it's not tyvek it's synthetic filter discs http://www.mycosupply.com/cgi-bin/shopper.cgi?preadd=action&key=SFDI01

i didn't pack it into the jars either, i just spooned it in loosely





The cakes were made wrong right from the beginning,they are prob to wet

Give this a look and follow it to a T
http://www.shroomery.org/forums/showflat.php/Number/11585613/fpart/1


Edited by Shea25 (05/21/10 08:34 PM)


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InvisibleSammy Agar
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Re: cease to show any progress after start.... [Re: travis873o]
    #12606566 - 05/21/10 09:14 PM (13 years, 8 months ago)

Quote:

travis873o said:
there is a dry layer of verm ontop (about quarter to half inch ontop). and for ratios of verm and water, i just soaked the verm until it could hold no more water (there was water in the bottom of container)  and then added 1/4 cup of verm i believe, (i followed direction from a book). then added the brf from what the book called for.

and it's not tyvek it's synthetic filter discs http://www.mycosupply.com/cgi-bin/shopper.cgi?preadd=action&key=SFDI01

i didn't pack it into the jars either, i just spooned it in loosely



I'm not following. Could go into a bit more depth on how exactly you made those? I mean it does sound all wrong, but I would just like to help you correct your wrong turns and get you back on the right path.  :peace:


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Offlinetravis873o
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Re: cease to show any progress after start.... [Re: Sammy Agar]
    #12608734 - 05/22/10 09:22 AM (13 years, 8 months ago)

i went according to "Psilocybin Mushroom Handbook" which called for 1/4 cup brf per jar and 1/2 cup verm per jar (plus extra for casing layer) and mix water w/ verm as I described above.  then mix in brf in after that.

the way they were stored however may have blocked the top of the containers from getting the proper GE could this have caused my problems?


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InvisibleSammy Agar
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Re: cease to show any progress after start.... [Re: travis873o]
    #12608897 - 05/22/10 10:09 AM (13 years, 8 months ago)

absolutely. they need air just like us. you must provide a way for GE.:smile:


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InvisibleJavadog
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Re: cease to show any progress after start.... [Re: Sammy Agar]
    #12609002 - 05/22/10 10:39 AM (13 years, 8 months ago)

Let me start to add the descriptions of all the things that I am doing
wrong, in the hopes of that I can help others do better....

1. I varied the density of my substrate in my second batch of
jars (yes, second.  I am a baby) and I found that the second batch
is colonizing slightly slower.

2. Of my first 12 jars, only one has shown contams after 2 weeks.
This was a jar in which the verm barrier did not come up the the
top of the jar....I noticed, belatedly, that the barrier was able
to shift around in the free space above the substrate.  I think that
this allowed contams (green mold) to get to through the barrier.

So, the jar with all the air at the top is problematic.  How did you
reach the substrate with a syringe?

Good luck, and post what works.


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Offlinetravis873o
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Re: cease to show any progress after start.... [Re: Javadog]
    #12609723 - 05/22/10 01:23 PM (13 years, 8 months ago)

how did i reach the substrate w/ the syringe? i just stuck it down throught the top layer of verm. the needle was long enough


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Offlinetravis873o
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Re: cease to show any progress after start.... [Re: travis873o]
    #12609749 - 05/22/10 01:28 PM (13 years, 8 months ago)

also it's still possible to turn out good results even though there wasn't proper GE previously right? i now have them able to breath.


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Offlinetravis873o
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Re: cease to show any progress after start.... [Re: travis873o]
    #12612021 - 05/22/10 09:58 PM (13 years, 8 months ago)

bump


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InvisibleSammy Agar
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Re: cease to show any progress after start.... [Re: travis873o]
    #12614026 - 05/23/10 12:28 PM (13 years, 8 months ago)

You should be fine with your syringes. As far as the jars, just leave 'em and see what they do.


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Offlinetravis873o
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Re: cease to show any progress after start.... [Re: travis873o] * 1
    #12616886 - 05/23/10 10:12 PM (13 years, 8 months ago)

so after letting the jars able to breathe, my mycelium has shown great progress, one jar's growth is even twice the size of what it used to be


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InvisibleSammy Agar
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Re: cease to show any progress after start.... [Re: travis873o] * 1
    #12617141 - 05/23/10 10:47 PM (13 years, 8 months ago)

See.....?:thumbup:


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