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Amazon Shop: ½ Pint Jars, Pressure Cooker

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OfflineJoker902
anti FOB

Registered: 01/09/03
Posts: 87
Loc: MI
Last seen: 11 years, 8 months
contamination )=
    #1261369 - 01/30/03 10:32 AM (14 years, 4 months ago)

"all words here by are from my neighbors manager @ kfc"
package was received on the 23th... during whole procedure latex gloves & masks were worn... jars were soaked in water & bleach over night, then boiled for over an hour in a canning cooker with substate & cooled over night... 11 jars were innoculated on the 25th... stored in a 40 gallon container @ an average of 72-73 degrees... as of today the 30th... i have a confirmed 7 contaminations... all from the Thailand Koh-Saumi syringe recieve... the last 4 jars were treasure coast... no signs of contamination, but also no signs of mycelium growth...

anyone know what possibly might went wrong?


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Offlineevilacid
PotHead

Registered: 01/26/03
Posts: 193
Loc: Amsterdam,Netherlands
Last seen: 14 years, 2 months
Re: contamination )= [Re: Joker902]
    #1261390 - 01/30/03 10:38 AM (14 years, 4 months ago)

were innoculated on the 25th... stored in a 40 gallon container @ an average of 72-73 degrees

anyone know what possibly might went wrong?







it needs to be at least 85 degress for it to start growth


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Offlineshirley knott
not my real name
 User Gallery
Registered: 11/11/02
Posts: 9,105
Loc: London
Last seen: 5 months, 19 days
Trusted Cultivator
Re: contamination )= [Re: evilacid]
    #1261406 - 01/30/03 10:42 AM (14 years, 4 months ago)

i'm gonna guess... contaminated syringe, and incubating too cold for fast growth.


--------------------
buh


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OfflineFunker
Master of Funk
Registered: 08/08/02
Posts: 41
Loc: East Berlin
Last seen: 14 years, 2 days
Re: contamination )= [Re: shirley knott]
    #1261662 - 01/30/03 11:55 AM (14 years, 4 months ago)

Quote:

it needs to be at least 85 degress for it to start growth




not true. my cakes start and grow fine at 60-65.. just a bit slower. sounds like the syringe coulda been contamed.


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InvisibleSixCee
keep rolling
Male

Registered: 06/12/02
Posts: 3,720
Loc: US, Chicago
Re: contamination )= [Re: Funker]
    #1261667 - 01/30/03 11:56 AM (14 years, 4 months ago)

Or maybe he innoculated too soon and fried the spores  :blush:.


--------------------
~~~~~~~~~~~~~~~~~~~~~~~~
-->The above statements may or not be true.
->Quote of the Moment :
"Yea. All bitches are whores who love sex." -Cubie
----> PMs checked daily.


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OfflineJoker902
anti FOB

Registered: 01/09/03
Posts: 87
Loc: MI
Last seen: 11 years, 8 months
Re: contamination )= [Re: SixCee]
    #1262042 - 01/30/03 02:25 PM (14 years, 4 months ago)

"all words were forwarded by my neighbors manager @ kfc"
i let the jars cool over 24 hours, so i dont think that was the problem... the other 4 jars were a different strain & they were sterelized, cooled, & innoculated @ the same time...


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OfflineJoker902
anti FOB

Registered: 01/09/03
Posts: 87
Loc: MI
Last seen: 11 years, 8 months
Re: contamination )= [Re: SixCee]
    #1262055 - 01/30/03 02:32 PM (14 years, 4 months ago)

Quote:

Or maybe he innoculated too soon and fried the spores  :blush:




if i had fried the spores, wouldnt nothing start to grow then? instead of contamination? i see green molds 


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Offlinedebianlinux
Myconerd - DBK
Male User Gallery

Folding@home Statistics
Registered: 12/09/02
Posts: 8,272
Loc: Over There
Last seen: 2 months, 11 days
Re: contamination )= [Re: Joker902]
    #1262090 - 01/30/03 02:48 PM (14 years, 4 months ago)

Quote:

Quote:

Or maybe he innoculated too soon and fried the spores  :blush:




if i had fried the spores, wouldnt nothing start to grow then? instead of contamination? i see green molds   




not a bad point but still not necessarily true. (I'm thinking you didn't heat-kill the spores since you cooled for 24hr) you DID insert a needle in there (prolly 4 times) and if the needle were contamed then obviously that would grow. not implying a contamed needle just rebutting your question.


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OfflineJoker902
anti FOB

Registered: 01/09/03
Posts: 87
Loc: MI
Last seen: 11 years, 8 months
Re: contamination )= [Re: debianlinux]
    #1262121 - 01/30/03 02:57 PM (14 years, 4 months ago)

not a bad point but still not necessarily true. (I'm thinking you didn't heat-kill the spores since you cooled for 24hr) you DID insert a needle in there (prolly 4 times) and if the needle were contamed then obviously that would grow. not implying a contamed needle just rebutting your question.




"all words were forwarded to me by my neighbors manager @ kfc"
well im thinking it was a contaminated needle, cuz the only jars that were contamined were the ones innoculated with the thailand strain... the other 4 jars look A-OK, & they were innoculated with treasure coast...


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InvisibleSixCee
keep rolling
Male

Registered: 06/12/02
Posts: 3,720
Loc: US, Chicago
Re: contamination )= [Re: Joker902]
    #1262433 - 01/30/03 04:33 PM (14 years, 4 months ago)

You didnt forget to sterilzie the needle did you?


--------------------
~~~~~~~~~~~~~~~~~~~~~~~~
-->The above statements may or not be true.
->Quote of the Moment :
"Yea. All bitches are whores who love sex." -Cubie
----> PMs checked daily.


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OfflineJoker902
anti FOB

Registered: 01/09/03
Posts: 87
Loc: MI
Last seen: 11 years, 8 months
Re: contamination )= [Re: SixCee]
    #1263057 - 01/30/03 08:23 PM (14 years, 4 months ago)

Quote:

You didnt forget to sterilzie the needle did you?




sterilized it with a flame until the needle was glowing red...


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Invisibledeprave
GrandmasterToker

Registered: 11/12/02
Posts: 366
Loc: DownUnder
Re: contamination )= [Re: Joker902]
    #1263125 - 01/30/03 08:42 PM (14 years, 4 months ago)

Yer neighbors manager  has his handsfull it sounds like  :cool: just tell him to take it easy and smoke some ganja  :laugh:


--------------------

NONE OF THE ABOVE IS TRUE I LIKE TO WRITE STORIES


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InvisibleRoadkill
Retired Shroomery Mod
Male User Gallery

Registered: 12/11/01
Posts: 22,598
Loc: Snoqualmie, Wa.
Trusted Cultivator
Re: contamination )= [Re: Joker902]
    #1263214 - 01/30/03 09:18 PM (14 years, 4 months ago)

Quote:

"all words here by are from my neighbors manager @ kfc"



Can I get a 3 pc meal to go?



--------------------
Laterz, Road

Who the hell you callin crazy?
You wouldn't know what crazy was if Charles Manson was eating froot loops on your front porch!


Brainiac said:
PM the names with on there names, that means they have mushrooms for sale.



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OfflineJoker902
anti FOB

Registered: 01/09/03
Posts: 87
Loc: MI
Last seen: 11 years, 8 months
Re: contamination )= [Re: Roadkill]
    #1264761 - 01/31/03 08:48 AM (14 years, 4 months ago)

Quote:

Quote:

"all words here by are from my neighbors manager @ kfc"



Can I get a 3 pc meal to go?






HAHAHHAHA dood my neighbor works on sunday, i get a bucket then! >=D


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Amazon Shop: ½ Pint Jars, Pressure Cooker

Mushrooms, Mycology and Psychedelics >> Mushroom Cultivation

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