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OfflineChasawah
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Can I transfer a colonizING jar to more jars?
    #12604215 - 05/21/10 01:37 PM (14 years, 7 months ago)

I have a few jars of mexa, can I open one and use it to start other jars. The jars will be PFtek on both ends...

Also, if I can, I have a jar at about 80%, and all the others are at around 50% which should I use?

Also, they all have stones forming already, can a stone be used directly to spawn more jars?



edit- feel like I need another Also in this thread.

Edited by Chasawah (05/21/10 01:38 PM)

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InvisibleMystery420
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Re: Can I transfer a colonizING jar to more jars? [Re: Chasawah]
    #12604235 - 05/21/10 01:41 PM (14 years, 7 months ago)

short answer NO you cant


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InvisibleFungal growth
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Re: Can I transfer a colonizING jar to more jars? [Re: Mystery420]
    #12604253 - 05/21/10 01:44 PM (14 years, 7 months ago)

got a long answer?
y cant he?
just wondering, if he can keep it sterile y wouldnt it work?
do pf cakes not lend themselves to transfers?

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OfflineGordy
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Re: Can I transfer a colonizING jar to more jars? [Re: Mystery420]
    #12604283 - 05/21/10 01:50 PM (14 years, 7 months ago)

Quote:

Mystery420 said:
short answer NO you cant




long answer, yes you can. but not like you asked.

once you have a jar 100% colonized, and consolidated if its pf tek, you can make a Poor Man's Mycelium Syringe which you then use this syringe to inoculate more jars.

i make my mycelium syringes, or grain lc, from grain. its a great way to get some lc quickly if you already have some spawn ready.


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OfflineGordy
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Re: Can I transfer a colonizING jar to more jars? [Re: Fungal growth]
    #12604289 - 05/21/10 01:51 PM (14 years, 7 months ago)

Quote:

Fungal growth said:
got a long answer?
y cant he?
just wondering, if he can keep it sterile y wouldnt it work?
do pf cakes not lend themselves to transfers?




you can't do a grain 2 grain type transfer with pf cakes because its simply too dense.


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InvisiblePluth
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Re: Can I transfer a colonizING jar to more jars? [Re: Chasawah]
    #12604311 - 05/21/10 01:55 PM (14 years, 7 months ago)

Quote:

Chasawah said:

Also, they all have stones forming already, can a stone be used directly to spawn more jars?





I think I am right in saying that you can use stones to spawn more jars.  It might have to be done using agar though.  I do know for a fact that dry stones can be rehydrated.


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OfflineChasawah
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Re: Can I transfer a colonizING jar to more jars? [Re: Gordy]
    #12604337 - 05/21/10 01:59 PM (14 years, 7 months ago)

So that poor mans is literally exactly what you do for a grain lc, just used on pf tek?

Is the karo critical or can I use sterilized water?

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Offlinemathias
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Re: Can I transfer a colonizING jar to more jars? [Re: Chasawah]
    #12604588 - 05/21/10 02:41 PM (14 years, 7 months ago)

Yeh, I did it with stalled jars that I thought I'd be clever with and spawn from.
It turned out OK, but I lost 2 jars to contam.
I would not advise it.
Much better to use 100% done jars to spawn from, or better yet, make a myc-syringe.


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Re: Can I transfer a colonizING jar to more jars? [Re: mathias]
    #12604658 - 05/21/10 02:52 PM (14 years, 7 months ago)

It wouldnt make sence to transfer uncolonized grain into an uncolonized jar, even if the first jar is halfway colonized or whatever.

The risk is there that you might get uncolonized grain from the first jar, which opens up more opportunity for contam.

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OfflineMad Hatter 2010
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Re: Can I transfer a colonizING jar to more jars? [Re: tekramrepus]
    #12604924 - 05/21/10 03:39 PM (14 years, 7 months ago)

To the OP

wait until your jars are 100% colonized (and maybe even consolidated)...then using one cake make a poor mans mycelium syringe.

You aren't using grains so you can't really do a g2g transfer.


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Grain LC's      Damion5050's Coir Tek      RR's Rye Grain Preperation      Strain Isolation

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OfflineChasawah
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Re: Can I transfer a colonizING jar to more jars? [Re: Mad Hatter 2010]
    #12604946 - 05/21/10 03:42 PM (14 years, 7 months ago)

[q
Quote:

Mad Hatter 2010 said:
To the OP

wait until your jars are 100% colonized (and maybe even consolidated)...then using one cake make a poor mans mycelium syringe.

You aren't using grains so you can't really do a g2g transfer.





Yeah I was thinking it would be fun trying to get a sample down into a sterilized pftek jar :rolleyes:

Does it have to be karo or can I use water?

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OfflineMad Hatter 2010
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Re: Can I transfer a colonizING jar to more jars? [Re: Chasawah]
    #12605131 - 05/21/10 04:15 PM (14 years, 7 months ago)

Quote:

Chasawah said:
[q
Quote:

Mad Hatter 2010 said:
To the OP

wait until your jars are 100% colonized (and maybe even consolidated)...then using one cake make a poor mans mycelium syringe.

You aren't using grains so you can't really do a g2g transfer.





Yeah I was thinking it would be fun trying to get a sample down into a sterilized pftek jar :rolleyes:

Does it have to be karo or can I use water?




http://www.shroomery.org/67/Poor-Mans-Mycelium-Syringe-Tek

A simple search would have answered your question but...Karo can be substituted with Honey...


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Grain LC's      Damion5050's Coir Tek      RR's Rye Grain Preperation      Strain Isolation

To my haters




Losers always whine about their best,Winners go home & fuck the prom queen!

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OfflineGordy
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Re: Can I transfer a colonizING jar to more jars? [Re: Chasawah]
    #12606118 - 05/21/10 07:44 PM (14 years, 7 months ago)

Karo is horrible, get Sugar In The Raw if you want to go with something you can get at any grocery store. Here's the Turbinado LC Tek posted by Agar.

About the Poor Man's Mycelium Syringe Tek as the Turbinado Tek states use one packet to 225ml of water. prepare this lc mix in lieu of the karo step in the tek. also, i would reccomend using an injection port on your lid. the cheapest way to do so is to use 100% silicone. it can be regular clear silicone or high temp RTV silicone, doesnt matter. the regular kind is rated for up to 400F. just poke a hole through your lid with a screwdriver or nail and put a nice glob over the hole, a little less than the size of a marble.

Quote:

Chasawah said:
So that poor mans is literally exactly what you do for a grain lc, just used on pf tek?

Is the karo critical or can I use sterilized water?




For this tek, yes it is critical that you use nutrients in your water. this is because you are not going to be able to get a whole lot of mycelium to break free from a very densely colonized BRF cake. so by adding nutrients to the water the small amount of mycelium in there will be able to grow and fill the syringe up with mycelium.


on another note... Mad Hatter, a simple read of the responses to his post and you would have seen i already gave him the same advice you did. :wink:


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OfflineChasawah
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Re: Can I transfer a colonizING jar to more jars? [Re: Gordy]
    #12606210 - 05/21/10 08:05 PM (14 years, 7 months ago)

Not to mention I asked specifically about water, not honey.

I know what Ill do, Ill make a small grain jar and throw some in there and let it colonize, and do a grain lc....

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OfflineRogerRabbitM
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Re: Can I transfer a colonizING jar to more jars? [Re: Chasawah]
    #12606295 - 05/21/10 08:24 PM (14 years, 7 months ago)

Quote:

Chasawah said:
I know what Ill do, Ill make a small grain jar and throw some in there and let it colonize, and do a grain lc....




That's a much better plan.  Even better would be to use the fully-colonized grain jar for grain to grain transfers.  Messing with brf cakes almost always ends in failure because it disturbs the vermiculite filter, allowing contaminants to come into contact with the substrate.
RR


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OfflineMad Hatter 2010
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Re: Can I transfer a colonizING jar to more jars? [Re: Chasawah]
    #12606374 - 05/21/10 08:39 PM (14 years, 7 months ago)

Quote:

Gordy said:
on another note... Mad Hatter, a simple read of the responses to his post and you would have seen i already gave him the same advice you did. :wink:




I actually happened to scroll past your 1st reply accidentally so...



Quote:

Chasawah said:
Not to mention I asked specifically about water, not honey.




I know you asked specifically about water,no honey...and again a simple search would have answered that question for you.I don't blame you,I am guilty of it too.But now whenever someone see's that I'm online in a forum,guess what?

I've also got another tab open where I soak up all the knowledge there is to be soaked up on this wonderful site.Make use of the tab labeled "Mushroom Info" at the top of the page,and make use of the search function.If you notice,I no longer start threads asking any questions I may have because of two reasons

1: I am not involved in a grow right now,though I am gathering the supplies needed.This isn't to say I haven't grown before as i have and I mastered the PF Tek and PF casings.Now,years later I am back and preparing for a bulk grow using a monotub.

2:All my questions that I did have,I found the answers by using the tab labeled "Mushroom Info" and by using the search function.And by reading threads that have to do with what I was questioning.


Quote:

Chasawah said:
I know what Ill do, Ill make a small grain jar and throw some in there and let it colonize, and do a grain lc....




There ya go!


--------------------
Grain LC's      Damion5050's Coir Tek      RR's Rye Grain Preperation      Strain Isolation

To my haters




Losers always whine about their best,Winners go home & fuck the prom queen!

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OfflineGordy
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Re: Can I transfer a colonizING jar to more jars? [Re: RogerRabbit]
    #12606450 - 05/21/10 08:52 PM (14 years, 7 months ago)

Quote:

RogerRabbit said:
Quote:

Chasawah said:
I know what Ill do, Ill make a small grain jar and throw some in there and let it colonize, and do a grain lc....




That's a much better plan.  Even better would be to use the fully-colonized grain jar for grain to grain transfers.  Messing with brf cakes almost always ends in failure because it disturbs the vermiculite filter, allowing contaminants to come into contact with the substrate.
RR




:thumbup: much better route. and then you can use the grain jar after it re-knits for more grain lc or for spawn. just make sure to get all standing water out of there. i found that half pints are easier to work with for grain lc. i usually make 4 very dense 20ml syringes from one half pint of wbs.

mad hatter : no cookie, thanks. i didn't mean anything by it, if anything great minds think alike!


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OfflineMad Hatter 2010
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Re: Can I transfer a colonizING jar to more jars? [Re: Gordy]
    #12606481 - 05/21/10 08:58 PM (14 years, 7 months ago)

It's all good gordy,I was actually trying to be humorous by offering you a cookie.I need to buy a damn mouse for my laptop already...I don't like the scroller on the built in mouse,gives me problems every time.


--------------------
Grain LC's      Damion5050's Coir Tek      RR's Rye Grain Preperation      Strain Isolation

To my haters




Losers always whine about their best,Winners go home & fuck the prom queen!

Extras: Filter Print Post Top
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