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OfflineAn Errant Egret
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Changes and MORE PICS [Re: An Errant Egret]
    #12299418 - 03/30/10 01:30 PM (14 years, 9 months ago)

I learned a few things and decided to make a bit of an alteration.

Actual mushrooms that may grow from pan A could potentially become too tall for the enclosure.  I opted to migrate the contents into a taller container.  As it just so happened, I couldn't find one that used the existing space very efficiently so I got two smaller containers instead and they fit almost perfectly into the refrigerator.  This is actually better IMO since it'll allow me to isolate environments and have differing growing conditions for my next generation, allowing me to identify better growth factors in a single generation.  W00t!

The new containers and the ventilation holes I drilled into the bottom of them (which I of course covered with micropore tape even though contamination is no longer a concern).  Since CO2 "sinks" and water vapor rises this should allow for a fairly steady supply of fresh air while still retaining the majority of the humidity.  Same theory as poking the holes at low points in the foil pans.


In the frige, ready to do some fruiting!


These are the closeups of what I'll now refer to as A(L) and A(R) - left and right, respectively - to differentiate the containers.  The casing layer became mixed with the substrate in the process of migration.  Material felt slightly warm to the touch compared to the surrounding environment, indicating that it's still alive at least.



One of my friends who I gave some of my extra culture jars to has had fruiting in a fully sterilized container with fully sterilized substrate and NO CASING LAYER in a refrigerator layout similar to mine (his took nearly 2 months to fruit).  This leads me to believe that the casing layer may not be required for introduction of microbes and may merely serve the purpose of retaining humidity in the substrate of "open" systems such as outdoor grows.  Closed but breathable systems where humidity remains fairly static (such as mine) may actually be hindered by a casing layer.  I plan to test this theory in my next generation, now that I have two fruiting chambers.

If my theory holds, then the popular practice of misting the top of the casing layer in outdoor grows may be virtually pointless since it's only the substrate humidity which matters.  It would also mean that casing with some kind of clear plastic would be ideal since it would retain moisture and allow light penetration, so long as there was enough airflow.

There's still no discernible pinning or primordia that I could see in the pan A material, though the casing material makes it somewhat difficult to tell.  The material had a MUCH stronger fungus odor that it did before, so I feel that's promising.  Based on my friend's experience, I may have to wait quite some time before I see any further growth.  The last two days have been between 30 and 50 degrees, so my frige has been fluctuating between about 31 and 47 degrees (I feel that ideal temperatures would be between 47 and 53 degrees).  Six hour light/dark cycles are still being used.  I'm still a bit curious about the "cold shock" that the Azures need, though... according to this graph of the humidity/temperatures in Astoria from September through November the temperature fluctuates quite a bit.  Does the shock need to be a one-time dip below 50, a multi-dip, or does it work best at a static temp below 50?

I'm still looking for advice on whether to grow the Malabar or Treasure Coast cubes that I have while I wait for the Azures.


--------------------
Ph. D. in particle physics, organic chemistry, molecular biology. Interdisciplinary minors in economics, psychology, infrastructure logistics. Over a dozen published works, two Nobel nominations, and accolades from twenty-seven world leaders.

So please, tell me in detail about the jack I don't know about shit that I might be privileged enough to glean a morsel of knowledge from your immense experience.

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InvisibleFlop Johnson
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Re: Changes and MORE PICS [Re: An Errant Egret]
    #12300372 - 03/30/10 04:06 PM (14 years, 9 months ago)

.

Edited by Flop Johnson (07/31/11 10:41 PM)

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OfflineAn Errant Egret
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Re: Changes and MORE PICS [Re: Flop Johnson]
    #12301287 - 03/30/10 06:48 PM (14 years, 9 months ago)

Quote:

MoeRon said:
Does your thermometer have a hi/lo feature? It will tell you the temperature fluctuations.

This is awesome, you are my new favorite shroomerite. :thumbup:




As of right now I'm simply using the adjuster knob in the frige itself to keep it at ~50 degrees.  I've unplugged the inline thermostat entirely, though I've left it running on batteries inside the refrigerator in order to check the temperature at a glance.  I'm not sure on the hi/lo feature - like any REAL man I've long since thrown away the instructions and I haven't really explored the functions beyond maintaining a minimum heat and minimum cool level.  I decided to eliminate the inline thermostat from controlling the frige in the first place because the cables interfered with the seal too much; being something of an ecologist I felt that running a refrigerator twice as much as needed was an unnecessary waste of energy.

I'm glad you like this, though personally I'd want to see some fruit before I declare someone a favorite  :wink:  One of my goals is to make my process easy to follow - I've found a TON of useful information on this forum, but I can't help but feel there's so much more since a lot of posts are so poorly written that they're virtually impossible to decipher.  I'm not trying to criticize anyone, just point out my motivation to achieve clarity.

I'm rereading the MMGG (3.72).  The MMGG is the first growing resource I ever read and I haven't even glanced at it in almost two years.  I'm finding a lot of updated information.  I understand that it's for cubes rather than azures, but there's a lot of helpful stuff in there that I'd simply forgotten about.  I'm thinking of modifying my fruiting chambers to allow better gas exchange and refining my humidity cycling system.  In any event it'll have to wait since I'm once again gearing up to leave town for a few days... once again, no updates for a while.


--------------------
Ph. D. in particle physics, organic chemistry, molecular biology. Interdisciplinary minors in economics, psychology, infrastructure logistics. Over a dozen published works, two Nobel nominations, and accolades from twenty-seven world leaders.

So please, tell me in detail about the jack I don't know about shit that I might be privileged enough to glean a morsel of knowledge from your immense experience.

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OfflineAn Errant Egret
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Advice? [Re: An Errant Egret]
    #12331035 - 04/04/10 06:22 PM (14 years, 9 months ago)

No pinning yet, but I have noticed something odd.  My substrate is getting "fuzzy" again.  Pan A had been sitting fully colonized in the incubator for nearly two months while I waited for weather conditions to improve and decided how to proceed.  The growth on the woodchips became thick and "rubbery" during that time, and this being my first grow I thought that was normal - it seemed like the woodchips were just becoming more thoroughly colonized.

The return of the "fuzz" indicates growth of fine structures, and I'm assuming that's a good thing.  If anyone can tell me more about what's going on I'd be very happy.  Also, any info on the "rubbery" aspect the mycellium took on would be appreciated - could it be because the condensed moisture on the pan's lid was "raining" on my substrate and repeatedly compressed the fine growth?  Was it a kind of stasis or "hibernation" state due to fully colonized substrate and inadequate fruiting conditions?


--------------------
Ph. D. in particle physics, organic chemistry, molecular biology. Interdisciplinary minors in economics, psychology, infrastructure logistics. Over a dozen published works, two Nobel nominations, and accolades from twenty-seven world leaders.

So please, tell me in detail about the jack I don't know about shit that I might be privileged enough to glean a morsel of knowledge from your immense experience.

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OfflineRogerRabbitM
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Re: Advice? [Re: An Errant Egret]
    #12331054 - 04/04/10 06:26 PM (14 years, 9 months ago)

Take it out of the refrigerator and put it in a bright window during the day.  They benefit from a warmer temp during the day than at night.

My suggestion is to use the whole thing as spawn to an outdoor bed as soon as it's warm enough in your area.
RR


--------------------
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OfflineAn Errant Egret
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Changed the lighting [Re: RogerRabbit]
    #12334365 - 04/05/10 09:53 AM (14 years, 9 months ago)

I want to avoid human interaction.  Since I'm out of town half the week, I'd like to "automate" the process as much as possible.  I'm more interested in refining the indoor hands-off procedure than I am in actually having the final product.  Attaining fruit is the goal of my TC grow.  Thanks for the idea, though.  I can certainly allow the frige to reach higher temps during lighted hours by simply putting a timer on it.  Also, keep in mind that I'm using four light/dark cycles at 6 hours each, not two at 12 hours.  Putting them in a window all day would mess with that cycle.  I'm testing a theory that faster cycling is beneficial - in many other organisms it's been shown that shorter but more frequent cycles of rest and activity accelerates metabolic processes.

As far as an outdoor bed, I'm not interested in that idea at the moment for various reasons.  Besides, I've got a rather plentiful stock of other colonized chips which I could dedicate to that task.

On a whim I decided to check my lighting and found that the bulb I was using was only 4100K temperature.  As far as I can figure via research, 5000K-7000K is optimal.  Being unable to find the right kind of tube lamp locally and not wanting to wait for one to be shipped I opted to use two 6500K CFL bulbs.


--------------------
Ph. D. in particle physics, organic chemistry, molecular biology. Interdisciplinary minors in economics, psychology, infrastructure logistics. Over a dozen published works, two Nobel nominations, and accolades from twenty-seven world leaders.

So please, tell me in detail about the jack I don't know about shit that I might be privileged enough to glean a morsel of knowledge from your immense experience.

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OfflineRogerRabbitM
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Re: Changed the lighting [Re: An Errant Egret]
    #12334507 - 04/05/10 10:31 AM (14 years, 9 months ago)

Quote:

As far as I can figure via research, 5000K-7000K is optimal.  Being unable to find the right kind of tube lamp locally and not wanting to wait for one to be shipped I opted to use two 6500K CFL bulbs.




Which IS the correct spectrum.
RR


--------------------
Download Let's Grow Mushrooms



semper in excretia sumus solim profundum variat

"I've never had a failed experiment.  I've only discovered 10,000 methods which do not work."
Thomas Edison

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OfflineAn Errant Egret
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Re: Changed the lighting [Re: RogerRabbit]
    #12566820 - 05/15/10 10:55 AM (14 years, 8 months ago)

It's all been destroyed.


--------------------
Ph. D. in particle physics, organic chemistry, molecular biology. Interdisciplinary minors in economics, psychology, infrastructure logistics. Over a dozen published works, two Nobel nominations, and accolades from twenty-seven world leaders.

So please, tell me in detail about the jack I don't know about shit that I might be privileged enough to glean a morsel of knowledge from your immense experience.

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Re: Changed the lighting [Re: An Errant Egret]
    #12566930 - 05/15/10 11:30 AM (14 years, 8 months ago)

Whyyyyy?!:mushroomtwirl:

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Invisibleteknix
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Re: Egret's indoor Azurescens [Re: An Errant Egret]
    #12567326 - 05/15/10 01:12 PM (14 years, 8 months ago)

:rofl2:

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InvisibleFlop Johnson
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Re: Changed the lighting [Re: An Errant Egret]
    #12572107 - 05/16/10 09:38 AM (14 years, 8 months ago)

.

Edited by Flop Johnson (07/31/11 10:40 PM)

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OfflineAn Errant Egret
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Why it was all destroyed [Re: Flop Johnson]
    #12595023 - 05/19/10 09:57 PM (14 years, 7 months ago)

Copied from my "Oven Sterilized TC" thread:

Wow, my life went NUTS in early April.  Wife and I had an argument.  Police were called.  FRANTIC destruction occurred.  It was a complete shitfeista, and I just narrowly avoided certain death by the skin of my teeth.  Cops and dogs walked the entire block and alley for a week stopping to sniff and check EVERYTHING.

As a result, I'm back down to being on the completely straight and narrow.  At this point in my life I can't afford to risk it (7 years down, only months to go...) and being on the precipice of certain obliteration put that into perspective.  So everything is gone.  I'm so sorry that I couldn't bring this to fruition for you guys and give hardcore picture evidence, but it works... try it!  I'm certain you have $30 sitting around somewhere (from not smoking for a week) and can spare it for science.  Or just wait I guess.

I will keep this thread updated.


--------------------
Ph. D. in particle physics, organic chemistry, molecular biology. Interdisciplinary minors in economics, psychology, infrastructure logistics. Over a dozen published works, two Nobel nominations, and accolades from twenty-seven world leaders.

So please, tell me in detail about the jack I don't know about shit that I might be privileged enough to glean a morsel of knowledge from your immense experience.

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InvisibleFlop Johnson
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Re: Why it was all destroyed [Re: An Errant Egret]
    #12596157 - 05/20/10 02:49 AM (14 years, 7 months ago)

.

Edited by Flop Johnson (07/31/11 10:40 PM)

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Invisiblewhyblameus
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Re: Why it was all destroyed [Re: Flop Johnson]
    #12596515 - 05/20/10 06:42 AM (14 years, 7 months ago)

damn man that sucks about the cops.

this is the first time i seen this thread i didnt read every word but at the beginning you said you thought you can do it 2 months start to finish.

as far as i know woodlovers need to be established for at least 6 months before they will fruit.and im really surprised no one had said that here already.

i also seen you say you had a friend with some that fruited and took 2 months to pin.
was that from when he started them fruiting or from the very beginning?

cause i would be amazed i have never heard of such speed with woodlovers.


anyways that sucks man sorry you didnt get to keep trying.:smirk:

at least your not in jail though:super:


--------------------
if you dont got no one to hate on feel free to hate on me!:tongue:

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InvisibleAlienscience
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Re: Why it was all destroyed [Re: whyblameus]
    #12601347 - 05/20/10 10:25 PM (14 years, 7 months ago)

I was going to ask for a spore print too.

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