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OfflineNolaNomad
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Ready for Agar
    #12597755 - 05/20/10 12:09 PM (14 years, 7 months ago)

Well, since I started growing, I've been collecting the equipment I need to do Agar work. I now have everything I need, and am going to do my first clone in the next day or two. Anyone have any suggestions or tips? I've only seen RR's video on it, and that only explains how to pour and work in front of a flow hood, which I don't have. I do, however, have a 100% success rate using my glove box (Never had a grain transfer contam). Any help would be appreciated.


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Disclaimer- The Author of this post assumes no responsibility for any information, factual or not, that he has posted herein. He does not grow mushrooms. Those pictures are fake. The information is all lies.
You heard me. I'm a liar. I only take drugs prescribed to me.

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InvisibleShea25
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Re: Ready for Agar [Re: NolaNomad]
    #12597774 - 05/20/10 12:15 PM (14 years, 7 months ago)

In a glove box its the same don't work over top of your agar dishes work quick and efficiently. Wear glove rub them down with 70% iso also wear tyvek sleeves

Edited by Shea25 (05/20/10 01:09 PM)

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OfflinehamloafM
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Re: Ready for Agar [Re: NolaNomad]
    #12597818 - 05/20/10 12:29 PM (14 years, 7 months ago)

Quote:

NolaNomad said:
Well, since I started growing, I've been collecting the equipment I need to do Agar work. I now have everything I need, and am going to do my first clone in the next day or two. Anyone have any suggestions or tips? I've only seen RR's video on it, and that only explains how to pour and work in front of a flow hood, which I don't have. I do, however, have a 100% success rate using my glove box (Never had a grain transfer contam). Any help would be appreciated.



Try open air procedures in your bathroom and think, "Jack be nimble, Jack be quick, Jack jump over the candlestick." Open air procedures are one of mycology's staples.


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OfflineBreakfast Crew
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Re: Ready for Agar [Re: hamloaf]
    #12597880 - 05/20/10 12:41 PM (14 years, 7 months ago)

Quote:

hamloaf said:
Quote:

NolaNomad said:
Well, since I started growing, I've been collecting the equipment I need to do Agar work. I now have everything I need, and am going to do my first clone in the next day or two. Anyone have any suggestions or tips? I've only seen RR's video on it, and that only explains how to pour and work in front of a flow hood, which I don't have. I do, however, have a 100% success rate using my glove box (Never had a grain transfer contam). Any help would be appreciated.



Try open air procedures in your bathroom and think, "Jack be nimble, Jack be quick, Jack jump over the candlestick." Open air sterile procedures are one of mycology's staples.




Fixed.

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OfflinehamloafM
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Re: Ready for Agar [Re: Breakfast Crew]
    #12598018 - 05/20/10 01:04 PM (14 years, 7 months ago)



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How I Get Stuff done. - My Reference Guide. - My Grows.

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InvisibleShea25
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Re: Ready for Agar [Re: hamloaf]
    #12598030 - 05/20/10 01:06 PM (14 years, 7 months ago)

Quote:

hamloaf said:
Quote:

NolaNomad said:
Well, since I started growing, I've been collecting the equipment I need to do Agar work. I now have everything I need, and am going to do my first clone in the next day or two. Anyone have any suggestions or tips? I've only seen RR's video on it, and that only explains how to pour and work in front of a flow hood, which I don't have. I do, however, have a 100% success rate using my glove box (Never had a grain transfer contam). Any help would be appreciated.



Try open air procedures in your bathroom and think, "Jack be nimble, Jack be quick, Jack jump over the candlestick." Open air procedures are one of mycology's staples.





Why would you tell someone to do agar in the open air.

He has a glove box so he should use it always for agar

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InvisibleShea25
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Re: Ready for Agar [Re: NolaNomad]
    #12598057 - 05/20/10 01:10 PM (14 years, 7 months ago)

Telling him to do open air agar when he has a glove box that he has had 100% success with is simply retarded, unless you know his mold spore count in his place or how clean it is I would not recommend ever doing agar in the open for a new comer especially when he has a glove box. If he listened to you he possibly could waste crap loads of agar and dishes and time

Open air is fine for inoculations but for , agar, G2G and spore printing glove box or flow hood all the way 

That has to be some of the worst advice I have heard, epically for a first time agar user

Edited by Shea25 (05/20/10 01:15 PM)

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OfflinehamloafM
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Re: Ready for Agar [Re: Shea25]
    #12598088 - 05/20/10 01:15 PM (14 years, 7 months ago)

Been there ~ done that, it worked

Glove boxes are constricting like a pair of tighty whities. Both will work for the pouring of agar. :rolleyes:
I was new when I learned open air procedures as I am sure you were and now OP is.

Edited by LiquidMyce (05/20/10 01:18 PM)

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InvisibleShea25
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Re: Ready for Agar [Re: hamloaf]
    #12598096 - 05/20/10 01:16 PM (14 years, 7 months ago)

Quote:

hamloaf said:
Been there ~ done that it worked

Glove boxes are constricting like a pair of tighty whities. Both will work for the pouring of agar. :rolleyes:




Ya for you not everyone has houses that have low spores counts or that are lucky. Its still bad advice to give out to people.

Your like the only person I have heard  telling a agar first timer to do it in the open air :facepalm:

Look how many precautions RR takes even with a flow hood? 

Open air Agar:thumbdown: telling people to do it for there first time:thumbdown:

Edited by Shea25 (05/20/10 01:19 PM)

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OfflinehamloafM
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Re: Ready for Agar [Re: Shea25]
    #12598435 - 05/20/10 02:16 PM (14 years, 7 months ago)

I was new to agar when I practiced it so why not "suggest" open air procedures to another. Sounds to me that you are implying that OP or any body new to agar pouring does not have the skill or intelligence to pour agar in a bathroom using open air procedures. Why are you trying to drag RR into this discussion. I don't see the man anywhere in this thread. As far as me "like" being the only person you have ever heard "telling" (suggesting actually) a first timer to "do it in open air"(I actually suggest open air procedures not plain open air, there is a difference) I was a first timer when I poured agar in open air using the procedure and figured if I can do it anybody can. Also as time goes on you will be finding the way I do things to be quite unique. I am a one of a kind.

To disagree is one thing but, shea25, please. Give the whole :thumbdown::thumbdown::facepalm: thing a rest. Again, :rolleyes:


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How I Get Stuff done. - My Reference Guide. - My Grows.

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InvisibleShea25
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Re: Ready for Agar [Re: hamloaf]
    #12598458 - 05/20/10 02:20 PM (14 years, 7 months ago)

Quote:

figured if I can do it anybody can




That's not the way it works, people can have unknown mold issues/dirty homes ect..

keep telling people to to open air agar and I will tell them to use glove boxes or flowhood, and you will see how many people come back to you with contaminated agar, What might work for you wont work for everyone else some people have pretty bad mold issues with there house. A glove box or flow hood solves this problem .

That's Like one person inoculating a jar with straight wild(not cleaned up) print and now advocates doing that.

Good for you open air agar works but for most people it wont, if you cant see the wrong in advocating open air agar instead of telling people to use a glove box or flowhood, I think you need to do a little more research.

I don't see why your going against the grain and telling people to go open air over a glove box and flowhood for agar that's simply bad information 

Its not like you coming up with some new experiential way to do agar, its just the wrong way to do agar correctly to maintain a high success rate .


I brought RR into this to explain even with a flowhood much care should be taken (procedures from his video) didn't think it was a crime to do so, that and I can use :facepalm: if I want to, it shows how ridiculous i think you advocating open air agar is. I don't need you telling me what I can and cannot use in my posts.

Other then that I'm not really up to debate this anymore it's plain and simple which is better and proven

Edited by Shea25 (05/20/10 02:39 PM)

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OfflineCloneufc
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Re: Ready for Agar [Re: Shea25]
    #12598761 - 05/20/10 03:10 PM (14 years, 7 months ago)

Open air agar! :thumbdown: :tard:

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InvisibleSet
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Re: Ready for Agar [Re: hamloaf]
    #12598784 - 05/20/10 03:13 PM (14 years, 7 months ago)

Quote:

hamloaf said:
Try open air procedures in your bathroom



bathrooms are one of the worst places


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InvisibleShea25
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Re: Ready for Agar [Re: Cloneufc]
    #12598836 - 05/20/10 03:20 PM (14 years, 7 months ago)

Quote:

Cloneufc said:
Open air agar! :thumbdown: :tard:




Agreed, happy someone finally came to back up this point

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OfflinehamloafM
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Re: Ready for Agar [Re: Shea25]
    #12598908 - 05/20/10 03:32 PM (14 years, 7 months ago)

Quote:


Quote:

Big Daddy Hamloaf said:figured if I can do it anybody can




That's not the way it works, people can have unknown mold issues/dirty homes ect..

keep telling people to to open air agar and I will tell them to use glove boxes or flowhood, and you will see how many people come back to you with contaminated agar, What might work for you wont work for everyone else some people have pretty bad mold issues with there house. A glove box or flow hood solves this problem .

That's Like one person inoculating a jar with straight wild(not cleaned up) print and now advocates doing that.

Good for you open air agar works but for most people it wont, if you cant see the wrong in advocating open air agar instead of telling people to use a glove box or flowhood, I think you need to do a little more research.

I don't see why your going against the grain and telling people to go open air over a glove box and flowhood for agar that's simply bad information 

Its not like you coming up with some new experiential way to do agar, its just the wrong way to do agar correctly to maintain a high success rate .


I brought RR into this to explain even with a flowhood much care should be taken (procedures from his video) didn't think it was a crime to do so, that and I can use :facepalm: if I want to, it shows how ridiculous i think you advocating open air agar is. I don't need you telling me what I can and cannot use in my posts.

Other then that I'm not really up to debate this anymore it's plain and simple which is better and proven



How many times are you going to edit this post then if you are so done with debate?


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How I Get Stuff done. - My Reference Guide. - My Grows.

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InvisibleShea25
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Re: Ready for Agar [Re: hamloaf]
    #12598919 - 05/20/10 03:34 PM (14 years, 7 months ago)

As much as I need to to get my point across

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Invisiblebadman
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Re: Ready for Agar [Re: Cloneufc]
    #12598945 - 05/20/10 03:38 PM (14 years, 7 months ago)

Quote:

Cloneufc said:
Open air agar! :tard:



Fixed

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OfflineShroomProphet
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Re: Ready for Agar [Re: badman]
    #12598971 - 05/20/10 03:43 PM (14 years, 7 months ago)

You gotta love this guy, been here 5 months and gives crappy advice out like candy. Whats next a video.


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OfflineRogerRabbitM
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Re: Ready for Agar [Re: ShroomProphet]
    #12599147 - 05/20/10 04:11 PM (14 years, 7 months ago)

A glovebox is second only to a flowhood.  The two nastiest rooms in any house are bathrooms and kitchens.  Kitchens have more molds due to food being stored there, and bathrooms have ungodly amounts of bacteria, not only from using the toilet, but shower curtains and stalls are literally bacteria breeding grounds, regardless of how much bleach one uses.

To the OP, look at the videos again.  The first one shows how to pour dishes, but there's also a strain isolation video and a master culture slant video.  The site has short clips which are free, taken from the full length DVD.
RR


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OfflinehamloafM
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Re: Ready for Agar [Re: Shea25]
    #12599409 - 05/20/10 04:51 PM (14 years, 7 months ago)

Quote:

ShroomProphet said:
You gotta love this guy, been here 5 months and gives crappy advice out like candy. Whats next a video.



I am a good dude. I mere suggested open air procedures as a means to an end in the first place and in no way was advocating open air procedures over the use of a glove-box or flowhood just arguing that it will work. Which is clearly implied in my first post appearing in this thread with the use of the word "Try"


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