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OfflineQuantumMeltdown
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Trich is killing me!
    #12236420 - 03/20/10 11:19 AM (14 years, 9 months ago)

For years Ive grown without any significant problems with trich. I might have had some show up after the 3rd flush at which point I would toss the casings. Now Im getting trich on almost 90% of my casings right about the point where its pinning on the first flush. Im almost positive this is not a case of having contaminated spawn. What I suspect is going on is that I have a very high concentration of spores in my room where the tubs are. I don't think its a case of not enough FAE either because I am using the same mono tubs that I have used for years with about 9 inch and a half holes in them stuffed with polyfill for breathing. I even tried switching from just letting them grow from the horse dung with not casing layer to using a ph adjusted casing layer of peat/verm this hasn't helped either. What do you folks suggest I do? Im thinking of tearing out the old nasty berber carpet I have in the room and completely sanitizing the room with lysol/rubbing alcohol maybe even running an air scrubber in there. I just can't keep going like this its so much work just to toss all these casings every time.

PS. Although I am reusing the tubs that have had infections in them I am washing them out with soap/bleach and then spraying them down and wiping with lysol prior to use. Could it be the tubs? I know that plastic is a bit porous in nature perhaps the lysol isn't enough to clean them completely and I need to start with new tubs?

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InvisibleSomeNewGuy
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Registered: 03/08/10
Posts: 215
Re: Trich is killing me! [Re: QuantumMeltdown]
    #12236872 - 03/20/10 01:06 PM (14 years, 9 months ago)

I want to say its the room. Carpet is definitely not your friend in this sport. Every step on that floor is doom, unless you have a steam-vac! Also any drapery or venetian blinds should be cleaned. New tubs cant hurt but a 10% bleach solution is usually good. i like to follow with 70% IPA (isopropyl).

I also think more air will help. Add a couple holes at a time while watching humidity levels once see a drop in humidity you know to tape the last few you made.


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Edited by SomeNewGuy (03/20/10 01:09 PM)

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OfflineQuantumMeltdown
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Re: Trich is killing me! [Re: SomeNewGuy]
    #12245521 - 03/21/10 10:14 PM (14 years, 9 months ago)

Bump anybody?

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InvisibleKhaos

Registered: 03/07/10
Posts: 183
Re: Trich is killing me! [Re: QuantumMeltdown]
    #12245578 - 03/21/10 10:25 PM (14 years, 9 months ago)

Out of curiosity what are spawning with? LC, Spore syringe?
Are you at some point using a spore syringe from a sponsor?
Or are you using your own collection of print/agar storage?

~Khaos


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"Surely all Americans have the right to give their money only to those causes which they support. But what kind of society has this created? A society where the ignorant reign. A society where enlightened must hold their tongues. A nation whose politicians must profess half-hearted devotion to an ancient fable or face the disastrous consequences of speaking their true mind." -Chris Mccandless 1987

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OfflineQuantumMeltdown
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Re: Trich is killing me! [Re: Khaos]
    #12245810 - 03/21/10 11:07 PM (14 years, 9 months ago)

Spawning using wbs grain to horse dung, some of the grains I use grain to grain transfer some of them are from syringes from sponsors. None of my grain jars are contamed prior to spawning to the dung I give them the smell test and they look very healthy.

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OfflineRogerRabbitM
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Re: Trich is killing me! [Re: QuantumMeltdown]
    #12245931 - 03/21/10 11:28 PM (14 years, 9 months ago)

In my experience, if you're getting trich at or near first flush, it's your spawn that's contaminated.  It takes longer than that for spores to land on your pasteurized substrate or casing, germinate and grow.  Washing the tubs with soap and water is fine.  I'd look at your  inoculant and/or sterile procedure when making spawn.
RR


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semper in excretia sumus solim profundum variat

"I've never had a failed experiment.  I've only discovered 10,000 methods which do not work."
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Offlinebasic360
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Re: Trich is killing me! [Re: RogerRabbit]
    #12246123 - 03/21/10 11:56 PM (14 years, 9 months ago)

Try adding captan at 5% by volume on your grain rinse and see how well that keeps the trich from germinating.  Let me know how it goes :smile:

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InvisibleSomeNewGuy
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Re: Trich is killing me! [Re: basic360]
    #12248463 - 03/22/10 11:54 AM (14 years, 9 months ago)

just searched "captan", no useful results please elaborate :smile:


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OfflineQuantumMeltdown
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Re: Trich is killing me! [Re: SomeNewGuy]
    #12248508 - 03/22/10 12:05 PM (14 years, 9 months ago)

it appears to be some type of fungicide. Where can one obtain it?

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Offlinebasic360
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Re: Trich is killing me! [Re: QuantumMeltdown]
    #12248559 - 03/22/10 12:16 PM (14 years, 9 months ago)

http://www.amazon.com/Orchids-Us-Inc-Captan-Fungicide/dp/B000YHB4HW

I've just been wanting to test this is a controlled experiment for awhile now after reading through some journals.

original: http://aem.asm.org/cgi/reprint/69/7/4190.pdf (pdf)

http://www.ncbi.nlm.nih.gov/pmc/articles/PMC165142/

It'll probably kill your mushrooms too though..worth a shot.  If you do get fruits, feed them to mice first to gauge cancer levels, then dose at your own risk :smile:

Edited by basic360 (03/22/10 12:40 PM)

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OfflineQuantumMeltdown
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Re: Trich is killing me! [Re: basic360]
    #12593161 - 05/19/10 04:15 PM (14 years, 7 months ago)

Update. I removed the carpet and started with new grain spawn inoculated with multi-spore injection. The rate of trich infections has drastically reduced from about 90% of my tubs infected before to maybe only about 5% now and there only minor spots of trich on the ones that do catch them and that's after the first flush. So its hard to say what it was, if it was the grain spawn or the room but things are much better now and that's all that matters.

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OfflineRogerRabbitM
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Re: Trich is killing me! [Re: QuantumMeltdown]
    #12597186 - 05/20/10 10:20 AM (14 years, 7 months ago)

It sounds like you've cleaned up your grain spawn technique.  I wouldn't suspect the bulk substrate.  Trich spores are really hard to get rid of once they infect your carpet, walls, curtains, etc.
RR


--------------------
Download Let's Grow Mushrooms



semper in excretia sumus solim profundum variat

"I've never had a failed experiment.  I've only discovered 10,000 methods which do not work."
Thomas Edison

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