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hal5000
Space Pirate



Registered: 04/03/10
Posts: 62
Loc:
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agar and substrains
#12587379 - 05/18/10 05:17 PM (14 years, 7 months ago) |
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Im currently reading through Stamets' The Mushroom Cultivator and I just read a part talking about sectoring. I just want to be sure I understand this corectly.
One mushroom drops thosands of spores, each having its own "sex" you could say. The hyphae from the spore contiunes on untill it finds a "mate" then become dikaryotic. So when you put some multispore into a nutrient rich petri dish and the mycelium begins to form it is actully creating hundreds of different little substrains? Is that right? Its talking about how one inoculation point can create both rhizomorphic and tomentose mycelium with rhizomorphic mycelium developing a great deal more primordia.
This being said, if one was to cut out just the rhizomorphic mycelium from a specimen, transfer it to a new petri dish, would the mycelium all be rhizomorphic or would it have both tomentose and rhizomorphic sectors just like G1 did?
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conoshoto
Mycologist In Training



Registered: 01/28/10
Posts: 563
Loc: The Other Side
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Re: agar and substrains [Re: hal5000]
#12587412 - 05/18/10 05:21 PM (14 years, 7 months ago) |
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The point of sectoring is to try and isolate one of those "pairs". Rhizomorphic traits are generally what you want so, ideally, one would sector and transfer until a "pure" plate is obtained with uniform growth type.
-------------------- "Life should not be a journey to the grave with the intention of arriving safely in a pretty and well preserved body, but rather to skid in broadside in a cloud of smoke, thoroughly used up, totally worn out, and loudly proclaiming "Wow! What a Ride!'" - Hunter S. Thompson
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wygram
Myconaut

Registered: 01/28/07
Posts: 573
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Re: agar and substrains [Re: conoshoto]
#12587489 - 05/18/10 05:34 PM (14 years, 7 months ago) |
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One transfer will not get you an isolated culture. The general rule is that in about 5-6 transfers to successive petri dishes you should be able to get an isolate. Multiple isolated should then be grown out to really explore each one's traits.
-------------------- Changing your mind is one of the best ways of finding out whether or not you still have one.
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conoshoto
Mycologist In Training



Registered: 01/28/10
Posts: 563
Loc: The Other Side
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Re: agar and substrains [Re: wygram]
#12587503 - 05/18/10 05:37 PM (14 years, 7 months ago) |
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"ideally one would sector and transfer until..."
I meant that it would obviously take more than one transfer. Depends on a lot of things for how many transfers exactly but I thought I made it clear.
-------------------- "Life should not be a journey to the grave with the intention of arriving safely in a pretty and well preserved body, but rather to skid in broadside in a cloud of smoke, thoroughly used up, totally worn out, and loudly proclaiming "Wow! What a Ride!'" - Hunter S. Thompson
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hal5000
Space Pirate



Registered: 04/03/10
Posts: 62
Loc:
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Re: agar and substrains [Re: conoshoto]
#12587618 - 05/18/10 05:57 PM (14 years, 7 months ago) |
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Oh ok. So once you have isolated a strain to purely rhizomorphic mycelium is it then possible to isolate the strain further for desired traits (i.e. yield, colonization time, contamination resistance)?
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andymc
cocoa beetles from zanzibar



Registered: 11/25/09
Posts: 2,395
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Re: agar and substrains [Re: hal5000]
#12587657 - 05/18/10 06:04 PM (14 years, 7 months ago) |
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In general, you've got everything correct, yes. When you cut a sector out of a petri, you're attempting to isolate some good (rhizo, for example) dikaryotic mycelium. It will probably take several transfers before you can successully isolate a genetic strain that is effectively a monoculture (an isolate, consisting of only one genetic strain)
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andymc
cocoa beetles from zanzibar



Registered: 11/25/09
Posts: 2,395
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Re: agar and substrains [Re: hal5000]
#12587680 - 05/18/10 06:08 PM (14 years, 7 months ago) |
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Quote:
hal5000 said: Oh ok. So once you have isolated a strain to purely rhizomorphic mycelium is it then possible to isolate the strain further for desired traits (i.e. yield, colonization time, contamination resistance)?
You can isolate several strains, and when you grow them out you can select based on those criteria (colonization time, fruiting ability, etc). If one grows some nice mushrooms, then you can clone the best one and make a master culture from that (in a slant, for example) and all your subsequent grows can be descendant from that culture.
-------------------- How I make spore prints
Trade List
My flow hood
If he asks me "Did you have a good time?" I'll say,
"Get the lights, Mr. Grim Reaper" -odds
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RogerRabbit
Bans for Pleasure


Registered: 03/26/03
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Re: agar and substrains [Re: andymc]
#12588186 - 05/18/10 07:39 PM (14 years, 7 months ago) |
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Quote:
andymc said: If one grows some nice mushrooms, then you can clone the best one and make a master culture from that (in a slant, for example) and all your subsequent grows can be descendant from that culture.
That's counterproductive. Save the original strains in the refrigerator and after you determine the best performing one(s), go back to that dish or slant and begin growing with much younger cell lines. RR
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andymc
cocoa beetles from zanzibar



Registered: 11/25/09
Posts: 2,395
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Quite right, sorry. It would obviously be redundant to clone a mushroom from a culture that's already an isolate.
-------------------- How I make spore prints
Trade List
My flow hood
If he asks me "Did you have a good time?" I'll say,
"Get the lights, Mr. Grim Reaper" -odds
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