Home | Community | Message Board

Cannabis Seeds - Original Sensible Seeds
This site includes paid links. Please support our sponsors.


Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Shop: Myyco.com Isolated Cubensis Liquid Culture For Sale   PhytoExtractum Buy Bali Kratom Powder   MagicBag.co All-In-One Bags That Don't Suck   Left Coast Kratom Buy Kratom Extract   Original Sensible Seeds Bulk Cannabis Seeds   Mushroom-Hut Substrate Bags   North Spore North Spore Mushroom Grow Kits & Cultivation Supplies   OlympusMyco.com No Unicorns Here—Just Quality Bags That Work

Jump to first unread post Pages: 1
Some of these posts are very old and might contain outdated information. You may wish to search for newer posts instead.
Invisiblehal5000
Space Pirate
 User Gallery


Registered: 04/03/10
Posts: 62
Loc: Flag
agar and substrains
    #12587379 - 05/18/10 05:17 PM (14 years, 7 months ago)

Im currently reading through Stamets' The Mushroom Cultivator and I just read a part talking about sectoring.  I just want to be sure I understand this corectly.

One mushroom drops thosands of spores, each having its own "sex" you could say.  The hyphae from the spore contiunes on untill it finds a "mate" then become dikaryotic.  So when you put some multispore into a nutrient rich petri dish and the mycelium begins to form it is actully creating hundreds of different little substrains?  Is that right?  Its talking about how one inoculation point can create both rhizomorphic and tomentose mycelium with rhizomorphic mycelium developing a great deal more primordia. 

This being said, if one was to cut out just the rhizomorphic mycelium from a specimen, transfer it to a new petri dish, would the mycelium all be rhizomorphic or would it have both tomentose and rhizomorphic sectors just like G1 did?

Extras: Filter Print Post Top
Invisibleconoshoto
Mycologist In Training
Male User Gallery


Registered: 01/28/10
Posts: 563
Loc: The Other Side
Re: agar and substrains [Re: hal5000]
    #12587412 - 05/18/10 05:21 PM (14 years, 7 months ago)

The point of sectoring is to try and isolate one of those "pairs". Rhizomorphic traits are generally what you want so, ideally, one would sector and transfer until a "pure" plate is obtained with uniform growth type.


--------------------
"Life should not be a journey to the grave with the intention of arriving safely in a pretty and well preserved body, but rather to skid in broadside in a cloud of smoke, thoroughly used up, totally worn out, and loudly proclaiming "Wow! What a Ride!'" - Hunter S. Thompson

Extras: Filter Print Post Top
Invisiblewygram
Myconaut

Registered: 01/28/07
Posts: 573
Trusted Cultivator
Re: agar and substrains [Re: conoshoto]
    #12587489 - 05/18/10 05:34 PM (14 years, 7 months ago)

One transfer will not get you an isolated culture. The general rule is that in about 5-6 transfers to successive petri dishes you should be able to get an isolate. Multiple isolated should then be grown out to really explore each one's traits.


--------------------
Changing your mind is one of the best ways of finding out whether or not you still have one.

Extras: Filter Print Post Top
Invisibleconoshoto
Mycologist In Training
Male User Gallery


Registered: 01/28/10
Posts: 563
Loc: The Other Side
Re: agar and substrains [Re: wygram]
    #12587503 - 05/18/10 05:37 PM (14 years, 7 months ago)

"ideally one would sector and transfer until..."

I meant that it would obviously take more than one transfer. Depends on a lot of things for how many transfers exactly but I thought I made it clear. :jah:


--------------------
"Life should not be a journey to the grave with the intention of arriving safely in a pretty and well preserved body, but rather to skid in broadside in a cloud of smoke, thoroughly used up, totally worn out, and loudly proclaiming "Wow! What a Ride!'" - Hunter S. Thompson

Extras: Filter Print Post Top
Invisiblehal5000
Space Pirate
 User Gallery


Registered: 04/03/10
Posts: 62
Loc: Flag
Re: agar and substrains [Re: conoshoto]
    #12587618 - 05/18/10 05:57 PM (14 years, 7 months ago)

Oh ok.  So once you have isolated a strain to purely rhizomorphic mycelium is it then possible to isolate the strain further for desired traits (i.e. yield, colonization time, contamination resistance)?

Extras: Filter Print Post Top
Invisibleandymc
cocoa beetles from zanzibar
Male User Gallery


Registered: 11/25/09
Posts: 2,395
Re: agar and substrains [Re: hal5000]
    #12587657 - 05/18/10 06:04 PM (14 years, 7 months ago)

In general, you've got everything correct, yes.  When you cut a sector out of a petri, you're attempting to isolate some good (rhizo, for example) dikaryotic mycelium.  It will probably take several transfers before you can successully isolate a genetic strain that is effectively a monoculture (an isolate, consisting of only one genetic strain)


--------------------
How I make spore prints
Trade List
My flow hood

If he asks me "Did you have a good time?" I'll say,
"Get the lights, Mr. Grim Reaper"  -odds

Extras: Filter Print Post Top
Invisibleandymc
cocoa beetles from zanzibar
Male User Gallery


Registered: 11/25/09
Posts: 2,395
Re: agar and substrains [Re: hal5000]
    #12587680 - 05/18/10 06:08 PM (14 years, 7 months ago)

Quote:

hal5000 said:
Oh ok.  So once you have isolated a strain to purely rhizomorphic mycelium is it then possible to isolate the strain further for desired traits (i.e. yield, colonization time, contamination resistance)?




You can isolate several strains, and when you grow them out you can select based on those criteria (colonization time, fruiting ability, etc).  If one grows some nice mushrooms, then you can clone the best one and make a master culture from that (in a slant, for example) and all your subsequent grows can be descendant from that culture.


--------------------
How I make spore prints
Trade List
My flow hood

If he asks me "Did you have a good time?" I'll say,
"Get the lights, Mr. Grim Reaper"  -odds

Extras: Filter Print Post Top
OfflineRogerRabbitM
Bans for Pleasure
Male User Gallery

Registered: 03/26/03
Posts: 42,214
Loc: Seattle
Last seen: 1 year, 10 months
Trusted Cultivator
OG Cultivator
Re: agar and substrains [Re: andymc]
    #12588186 - 05/18/10 07:39 PM (14 years, 7 months ago)

Quote:

andymc said:
If one grows some nice mushrooms, then you can clone the best one and make a master culture from that (in a slant, for example) and all your subsequent grows can be descendant from that culture.




That's counterproductive.  Save the original strains in the refrigerator and after you determine the best performing one(s), go back to that dish or slant and begin growing with much younger cell lines.
RR


--------------------
Download Let's Grow Mushrooms



semper in excretia sumus solim profundum variat

"I've never had a failed experiment.  I've only discovered 10,000 methods which do not work."
Thomas Edison

Extras: Filter Print Post Top
Invisibleandymc
cocoa beetles from zanzibar
Male User Gallery


Registered: 11/25/09
Posts: 2,395
Re: agar and substrains [Re: RogerRabbit]
    #12590535 - 05/19/10 06:29 AM (14 years, 7 months ago)

Quite right, sorry.  It would obviously be redundant to clone a mushroom from a culture that's already an isolate.


--------------------
How I make spore prints
Trade List
My flow hood

If he asks me "Did you have a good time?" I'll say,
"Get the lights, Mr. Grim Reaper"  -odds

Extras: Filter Print Post Top
Jump to top Pages: 1

Shop: Myyco.com Isolated Cubensis Liquid Culture For Sale   PhytoExtractum Buy Bali Kratom Powder   MagicBag.co All-In-One Bags That Don't Suck   Left Coast Kratom Buy Kratom Extract   Original Sensible Seeds Bulk Cannabis Seeds   Mushroom-Hut Substrate Bags   North Spore North Spore Mushroom Grow Kits & Cultivation Supplies   OlympusMyco.com No Unicorns Here—Just Quality Bags That Work


Similar ThreadsPosterViewsRepliesLast post
* The secret of Agar JetBlackNinja 1,934 11 12/17/03 05:23 PM
by llamaboy
* rhizomorphic isolate going back to linear / tomentose ohmatic 4,770 15 01/19/05 01:47 PM
by tripndicular
* How to make agar 2 agar transfer? esp 6,944 7 01/08/04 11:08 AM
by YidakiMan
* transferring agar meatman 2,575 3 10/02/02 01:13 PM
by tripndicular
* agar isolations vs multispore = no contest for comparison? mavix 1,877 2 02/17/04 09:10 PM
by simplemachine
* agar to agar transfer ohmatic 16,335 11 01/12/21 09:00 PM
by bodhisatta
* using agar? downforpot 2,318 6 12/02/01 07:00 PM
by Anno
* Agar questions-I may have goofed blahblahblah 3,392 6 01/11/02 02:58 PM
by ar393

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: Shroomism, george castanza, RogerRabbit, veggie, mushboy, fahtster, LogicaL Chaos, 13shrooms, hamloaf, cronicr, Stipe-n Cap, Pastywhyte, bodhisatta, Tormato, Land Trout, A.k.a
612 topic views. 18 members, 71 guests and 20 web crawlers are browsing this forum.
[ Show Images Only | Sort by Score | Print Topic ]
Search this thread:

Copyright 1997-2025 Mind Media. Some rights reserved.

Generated in 0.022 seconds spending 0.005 seconds on 12 queries.