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OfflineAmphiprion
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Registered: 04/16/10
Posts: 154
Loc: Mindeapoarctica
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Stalled WBS jar Mycelium extraction
    #12571765 - 05/16/10 07:12 AM (14 years, 8 months ago)

Hi guys.

Ive been looking for Agar's tek on extracting mycelium out of stalled jars but can't find it again.

I currently Have two stalled WBS jars of which I have removed uncolonized WBS and injected sterile water onto the colonized WBS, as I would like to extract mycelium laden water out when im done to colonize fresh PF cakes I have made.

Please could you advise on the best way to do this as I feel that as it stands, The water will  not contain myc after a few hours of soaking (never heard of dunk water containing myc).

Any help please.

Jars currently still in glovebox with sterile water injected to get myc out. think i need to break up the myc a bit.


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Amphi

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OfflineRogerRabbitM
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Registered: 03/26/03
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Re: Stalled WBS jar Mycelium extraction [Re: Amphiprion]
    #12571829 - 05/16/10 07:40 AM (14 years, 8 months ago)

If the jar stalled, there's a reason, such as bacterial contamination.  Start over and use a healthy jar.
RR


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OfflineAmphiprion
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Registered: 04/16/10
Posts: 154
Loc: Mindeapoarctica
Last seen: 11 years, 9 months
Re: Stalled WBS jar Mycelium extraction [Re: RogerRabbit]
    #12572087 - 05/16/10 09:29 AM (14 years, 8 months ago)

gotta order a new MS syringe. Dam.

Thanks RR


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Amphi

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OfflineAmphiprion
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Registered: 04/16/10
Posts: 154
Loc: Mindeapoarctica
Last seen: 11 years, 9 months
Re: Stalled WBS jar Mycelium extraction [Re: Amphiprion]
    #12589834 - 05/19/10 12:18 AM (14 years, 7 months ago)

So from the same set of jars that seemed stalled, I shaked three of them.

The shaked jars have done extremely well with recolonization and are at 50%.

still quite slow compared to other grows..

Amphi


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Amphi

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