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Offlinestaneh
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Inoculating Rye Berries w/ BRF Mycelia?
    #12589435 - 05/18/10 10:56 PM (14 years, 7 months ago)

Hey all-

I have 6 qt jars filled 3/4 with rye berries that were soaked for 16 hours, strained, simmered for 45 mins, rinsed, strained again, then pressure cooked @ 15 psi for 90 mins. they've been cooling for a good five hours but i'm gonna let them go overnight. they're hanging out in the room my incubator is in (a sauna:p)

i'm wondering...i have some perfectly fine mycelial growth on some brf cakes, but i want to switch to rye, so could i potentially cut out the myc from the brf jar and drop it in to the rye jars to inoculate?

if so, give me some detailed instructions please

thanks!!!

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Invisibleconoshoto
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Re: Inoculating Rye Berries w/ BRF Mycelia? [Re: staneh]
    #12589469 - 05/18/10 11:02 PM (14 years, 7 months ago)

Yeah, it'll work, but I would recommend making a glove box to do it in. And room temp. is fine for incubation, higher temps increase your chance of contam.


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"Life should not be a journey to the grave with the intention of arriving safely in a pretty and well preserved body, but rather to skid in broadside in a cloud of smoke, thoroughly used up, totally worn out, and loudly proclaiming "Wow! What a Ride!'" - Hunter S. Thompson

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Offlineaudiophoenix
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Re: Inoculating Rye Berries w/ BRF Mycelia? [Re: conoshoto]
    #12589487 - 05/18/10 11:06 PM (14 years, 7 months ago)

I would also Break the cake in half and take a sample out of the center of the cake and be very sterile about it.


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Offlinestaneh
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Re: Inoculating Rye Berries w/ BRF Mycelia? [Re: conoshoto]
    #12589491 - 05/18/10 11:07 PM (14 years, 7 months ago)

its around 72-76 throughout the day...that seems like it should be okay

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OfflineTheZephyr
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Re: Inoculating Rye Berries w/ BRF Mycelia? [Re: staneh]
    #12589508 - 05/18/10 11:09 PM (14 years, 7 months ago)

what youre talking about is called a grain to grain transfer (g2g), if you want detailed instructions type g2g in the search box at top right of screen

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Invisibleconoshoto
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Re: Inoculating Rye Berries w/ BRF Mycelia? [Re: staneh]
    #12589515 - 05/18/10 11:11 PM (14 years, 7 months ago)

Yeah, that's a good temp :thumup:


--------------------
"Life should not be a journey to the grave with the intention of arriving safely in a pretty and well preserved body, but rather to skid in broadside in a cloud of smoke, thoroughly used up, totally worn out, and loudly proclaiming "Wow! What a Ride!'" - Hunter S. Thompson

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Offlinestaneh
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Re: Inoculating Rye Berries w/ BRF Mycelia? [Re: conoshoto]
    #12589528 - 05/18/10 11:14 PM (14 years, 7 months ago)

would the same apply to a clump of mycelia from a cake that already produced a couple mushies? would that contaminate easier/not as easy, colonize faster/worse?

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Invisibleconoshoto
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Re: Inoculating Rye Berries w/ BRF Mycelia? [Re: staneh]
    #12589544 - 05/18/10 11:16 PM (14 years, 7 months ago)

You could but it would increase contam chances... if anything you could (in a glove box) cut a piece out of the middle of a fresh shroom, put it in a ziplock bag, wait a few days for it to get fuzzy with myc, and then put into a grain jar... but that is essentially making a clone. I wouldn't take a piece off of a cake that was already fruiting though :shrug:


--------------------
"Life should not be a journey to the grave with the intention of arriving safely in a pretty and well preserved body, but rather to skid in broadside in a cloud of smoke, thoroughly used up, totally worn out, and loudly proclaiming "Wow! What a Ride!'" - Hunter S. Thompson

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Offlinestaneh
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Re: Inoculating Rye Berries w/ BRF Mycelia? [Re: conoshoto]
    #12589586 - 05/18/10 11:23 PM (14 years, 7 months ago)

but some myc from a colonizing cake would be okay?

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OfflineBreakfast Crew
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Re: Inoculating Rye Berries w/ BRF Mycelia? [Re: staneh]
    #12589590 - 05/18/10 11:24 PM (14 years, 7 months ago)

Quote:

staneh said:
would the same apply to a clump of mycelia from a cake that already produced a couple mushies? would that contaminate easier/not as easy, colonize faster/worse?




I asked that very question a year ago.  The best answer I got was to use the "Youngest" mycelium possible.  You don't want weak (old) mycelium in its later flushes or spent mycelium.

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Invisibleconoshoto
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Re: Inoculating Rye Berries w/ BRF Mycelia? [Re: staneh]
    #12589604 - 05/18/10 11:26 PM (14 years, 7 months ago)

Yeah, because it hasn't been introduced to outside contams yet. It would be much safer imo than a cake with weak myc from fruiting.


--------------------
"Life should not be a journey to the grave with the intention of arriving safely in a pretty and well preserved body, but rather to skid in broadside in a cloud of smoke, thoroughly used up, totally worn out, and loudly proclaiming "Wow! What a Ride!'" - Hunter S. Thompson

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Offlinestaneh
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Re: Inoculating Rye Berries w/ BRF Mycelia? [Re: conoshoto]
    #12590573 - 05/19/10 06:49 AM (14 years, 7 months ago)

so what should i do, just knife/spoon out the myc from the jar, drop it in the rye jars, and shake?

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InvisibleDoc_T
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Re: Inoculating Rye Berries w/ BRF Mycelia? [Re: staneh]
    #12590598 - 05/19/10 07:03 AM (14 years, 7 months ago)

Carefully. Under sterile conditions. And do more than one.


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You make it all possible. Doesn't it feel good?

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