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Offlineavaldez314
Silent Bob
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Registered: 04/26/10
Posts: 136
Last seen: 10 years, 10 months
[Pics] Liquid culture from agar, need suggestions...
    #12586351 - 05/18/10 03:03 PM (14 years, 7 months ago)

Hi, one week ago I transferred several pieces of colonized agar to a liquid medium (water + 4% honey), it's my first attempt to create a liquid culture.

I don't know if I should let the mycelium grow into the liquid's surface, or if I should use my magnetic stirrer to mix it with the liquid.

Also, how can I know when the liquid culture is ready to be used?

Any suggestion will be very appreciated :tongue:


Upper view (mycelium is floating in the surface):


Font view (I'm not sure what is in the bottom of the jar):


Upper zoom:


Bottom zoom:


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Invisiblebadman
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Registered: 06/14/06
Posts: 4,039
Re: [Pics] Liquid culture from agar, need suggestions... [Re: avaldez314]
    #12586503 - 05/18/10 03:24 PM (14 years, 7 months ago)

Looking good!
:awethumb:

That creaming of myc happens sometimes. You should stir it or shake by hand daily to prevent the myc aggregating.

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InvisibleBuddha420
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Registered: 05/15/10
Posts: 411
Loc: Mobil, Alabama
Re: [Pics] Liquid culture from agar, need suggestions... [Re: badman]
    #12586809 - 05/18/10 04:03 PM (14 years, 7 months ago)

whats the purpose of this method? better strain isolation?


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Offlinepurpleprint90
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Registered: 05/17/10
Posts: 22
Last seen: 14 years, 3 months
Re: [Pics] Liquid culture from agar, need suggestions... [Re: Buddha420]
    #12586895 - 05/18/10 04:12 PM (14 years, 7 months ago)

Theres only one way to know if its ok for sure. You gotsta knock somthing up.

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Invisiblebadman
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Registered: 06/14/06
Posts: 4,039
Re: [Pics] Liquid culture from agar, need suggestions... [Re: Buddha420]
    #12587171 - 05/18/10 04:48 PM (14 years, 7 months ago)

Quote:

Buddha420 said:
whats the purpose of this method? better strain isolation?



Live liquid inoculum. Thats it.

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Offlineavaldez314
Silent Bob
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Registered: 04/26/10
Posts: 136
Last seen: 10 years, 10 months
Re: [Pics] Liquid culture from agar, need suggestions... [Re: badman]
    #12596432 - 05/20/10 06:11 AM (14 years, 7 months ago)

Quote:

badman said:
Looking good!
:awethumb:

That creaming of myc happens sometimes. You should stir it or shake by hand daily to prevent the myc aggregating.





I'll take you suggestion and give it a heavy stirring session :-)

Is there any visual hint to know when the LC is ready to be used?  (I don't know, maybe the medium should look cloudy or dense?)


--------------------
Sclerotia producing print WANTED!

P. Mexicana/A, P. galindoi/ATL#7 or P. tampanensis

if you are interested in a trade please PM me!

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Offlinepurpleprint90
Stranger

Registered: 05/17/10
Posts: 22
Last seen: 14 years, 3 months
Re: [Pics] Liquid culture from agar, need suggestions... [Re: avaldez314]
    #12596967 - 05/20/10 09:27 AM (14 years, 7 months ago)

When it comes to lc you can use it when ever you want, but the more mycelium you have the better.

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Offlineafrosheen
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Registered: 03/06/10
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Re: [Pics] Liquid culture from agar, need suggestions... [Re: Buddha420]
    #12597335 - 05/20/10 10:50 AM (14 years, 7 months ago)

Quote:

Buddha420 said:
whats the purpose of this method? better strain isolation?




Like the other guy said, the myc is live in this state, and it grows alot faster when inoculated to your substrate of choice. Spores take time to germinate in a substrate, LC is ready to rock when it hits.


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