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OfflineBeerFoam
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CAn i use jello as agar?
    #12581504 - 05/17/10 07:57 PM (14 years, 8 months ago)

Can i use Jello as agar or anything else that easy assessable, as agar?

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InvisibleBuddha420
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Registered: 05/15/10
Posts: 411
Loc: Mobil, Alabama
Re: CAn i use jello as agar? [Re: BeerFoam]
    #12581511 - 05/17/10 07:57 PM (14 years, 8 months ago)

just order it online...its not that inaccessible


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Psychedelic Salon - changing our minds, one thought at a time

Religion - George Carlin, R.I.P.

BLUE CHAMPS!!! Chelsea FC - 09/10

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OfflineBeerFoam
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Re: CAn i use jello as agar? [Re: Buddha420]
    #12581514 - 05/17/10 07:58 PM (14 years, 8 months ago)

yea i just hate waiting

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InvisibleBuddha420
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Registered: 05/15/10
Posts: 411
Loc: Mobil, Alabama
Re: CAn i use jello as agar? [Re: BeerFoam]
    #12581544 - 05/17/10 08:06 PM (14 years, 8 months ago)

cannot answer your question thought, i've never used jello...try it, post here, maybe it'll work =)


--------------------
Psychedelic Salon - changing our minds, one thought at a time

Religion - George Carlin, R.I.P.

BLUE CHAMPS!!! Chelsea FC - 09/10

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InvisibleFoxFire
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Re: CAn i use jello as agar? [Re: BeerFoam]
    #12581578 - 05/17/10 08:13 PM (14 years, 8 months ago)

Quote:

BeerFoam said:
yea i just hate waiting




Then this is not the hobby for you.


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Unless someone like you cares a whole awful lot,
Nothing is going to get better.  It's not.

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InvisibleFoxFire
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Re: CAn i use jello as agar? [Re: BeerFoam]
    #12581623 - 05/17/10 08:23 PM (14 years, 8 months ago)

sorry didn't meant sound like a dick,
but that shit is probably full of preservatives and chemicals. :thumbdown:

Here:

RR Said-
Jello has been tried dozens of times over the years.  You can find the results with a search.  It isn't an agar substitute.  The mycelium will feed on the gelatin, turning it back to liquid.  It will also begin to rot at room temperature, leading to bacterial contamination.  I'm not aware of a single success story.  Sorry.


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Unless someone like you cares a whole awful lot,
Nothing is going to get better.  It's not.

Edited by FoxFire (05/17/10 08:25 PM)

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OfflineBeerFoam
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Re: CAn i use jello as agar? [Re: FoxFire]
    #12581777 - 05/17/10 08:47 PM (14 years, 8 months ago)

kk looks like ill order some agar 2night

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Invisibleanonjon
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Re: CAn i use jello as agar? [Re: BeerFoam]
    #12581787 - 05/17/10 08:49 PM (14 years, 8 months ago)

they sell agar at oriental supermarkets and in the oriental section of fancy grocery stores.


it's called agar agar.

there's lots of recipes. mine is one half stick of agar, 1 tablespoon corn syrup, 1 teaspoon of potato starch ..stirred into 1 cup of boiling water

substitute the sugar or starch with wutever u have available.



--------------------
The above post is fictional, hypothetical, or downright nonsensical.


:moon:  :moon:  :moon:  :moon:    :moon:

Edited by anonjon (05/17/10 08:54 PM)

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OfflineGrumpyorc
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Re: CAn i use jello as agar? [Re: anonjon]
    #12581858 - 05/17/10 09:01 PM (14 years, 8 months ago)

Here ya go man This is what I have always used and I have never had a problem. I isolate and clone and have never even seen a real agar plate
BRF agar substitute

edit- I leave out the layer of tyvek and have no problems. My house is clean tho. I'm obsessive compulsive BAD.


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Edited by Grumpyorc (05/17/10 09:02 PM)

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InvisibleDoc_T
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Re: CAn i use jello as agar? [Re: Grumpyorc]
    #12581991 - 05/17/10 09:21 PM (14 years, 8 months ago)

Quote:

Grumpyorc said:
Here ya go man This is what I have always used and I have never had a problem. I isolate and clone and have never even seen a real agar plate
BRF agar substitute

edit- I leave out the layer of tyvek and have no problems. My house is clean tho. I'm obsessive compulsive BAD.




Could you use that stuff in petri dishes? It would be handy to fill, seal, sterilize.


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You make it all possible. Doesn't it feel good?

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OfflineBeerFoam
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Re: CAn i use jello as agar? [Re: Doc_T]
    #12582088 - 05/17/10 09:36 PM (14 years, 8 months ago)

well to late i had to buy gypsum and petri dishes anyways, so i just bought, dry malt extract, and dextrose, cause sporeworks agar is sold out.

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InvisibleDoc_T
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Re: CAn i use jello as agar? [Re: BeerFoam]
    #12582117 - 05/17/10 09:40 PM (14 years, 8 months ago)

Quote:

BeerFoam said: so i just bought, dry malt extract, and dextrose, cause sporeworks agar is sold out.



You didn't get agar yet? Go get it at a health food store.


--------------------
You make it all possible. Doesn't it feel good?

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OfflineBeerFoam
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Re: CAn i use jello as agar? [Re: Doc_T]
    #12582127 - 05/17/10 09:41 PM (14 years, 8 months ago)

cant dry malt extract be used for cloning and isolation 2?

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InvisibleDoc_T
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Re: CAn i use jello as agar? [Re: BeerFoam]
    #12582139 - 05/17/10 09:43 PM (14 years, 8 months ago)

:picard: homework fail.


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You make it all possible. Doesn't it feel good?

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OfflineBeerFoam
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Re: CAn i use jello as agar? [Re: Doc_T]
    #12582185 - 05/17/10 09:49 PM (14 years, 8 months ago)

dang i did fail lol -.-

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OfflineBeerFoam
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Re: CAn i use jello as agar? [Re: BeerFoam]
    #12582221 - 05/17/10 09:55 PM (14 years, 8 months ago)

k fixed :P

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OfflineGrumpyorc
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Re: CAn i use jello as agar? [Re: Doc_T]
    #12585245 - 05/18/10 12:04 PM (14 years, 7 months ago)

Quote:

Doc_T said:
Quote:

Grumpyorc said:
Here ya go man This is what I have always used and I have never had a problem. I isolate and clone and have never even seen a real agar plate
BRF agar substitute

edit- I leave out the layer of tyvek and have no problems. My house is clean tho. I'm obsessive compulsive BAD.




Could you use that stuff in petri dishes? It would be handy to fill, seal, sterilize.



I dunno I don't have any petri dishes. I don't see why not. Seems like it would work
I was thinking about adding a little verm and tryin to get some sterile pins. That would be cool eh? The jars have GE so it seems to me like it may work


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Invisibleanonjon
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Re: CAn i use jello as agar? [Re: Grumpyorc]
    #12586571 - 05/18/10 03:33 PM (14 years, 7 months ago)

Quote:

Grumpyorc said:
I was thinking about adding a little verm and tryin to get some sterile pins. That would be cool eh? The jars have GE so it seems to me like it may work




yeah that would be kinda cool if u had a method of growing small pins to transfer to grain jars.


--------------------
The above post is fictional, hypothetical, or downright nonsensical.


:moon:  :moon:  :moon:  :moon:    :moon:

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OfflineGrumpyorc
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Re: CAn i use jello as agar? [Re: anonjon]
    #12587834 - 05/18/10 06:31 PM (14 years, 7 months ago)

hmmm well I have a strain that is especially Co2 tolerant. Maybe I'll give it a shot. Problem is the camera :p Will need to get a camera for a proper log


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OfflineShaggy Shaman
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Re: CAn i use jello as agar? [Re: Grumpyorc]
    #12588598 - 05/18/10 08:57 PM (14 years, 7 months ago)

Quote:

Grumpyorc said:
Here ya go man This is what I have always used and I have never had a problem. I isolate and clone and have never even seen a real agar plate
BRF agar substitute

edit- I leave out the layer of tyvek and have no problems. My house is clean tho. I'm obsessive compulsive BAD.




I hadn't seen this tek before. I bet I can get some good use from it. Thanks Grumpy for pointing it out to us!


--------------------
"Disbelief in magic can force a poor soul into believing in government and business."
— Tom Robbins

“The true value of a human being can be found in the degree to which he has attained liberation from the self"
-- Albert Einstein

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Invisibleambargh
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Re: CAn i use jello as agar? [Re: Shaggy Shaman]
    #12588739 - 05/18/10 09:15 PM (14 years, 7 months ago)

I just ordered some malt extract from a brewer supply store (5 bucks) and picked up some agar-agar from the korean supermarket (2 bucks) and make my own. Works pretty good.




Malt Extract Agar (3%)(MEA)(General Microbiology)(FDA M93)

30 g Malt extract
20 g Agar
1 L Distilled water

Boil to dissolve ingredients. Avoid overheating, which causes softening of agar and darkening of medium color. Autoclave 15 min at 121°C. Dispense 20-25 ml into sterile 15 x 100 mm petri dishes. Final pH, 5.5 ± 0.2.

This medium is recommended as a general maintenance medium. [1]


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"The fact that we live at the bottom of a deep gravity well, on the surface of a gas covered planet going around a nuclear fireball 90 million miles away and think this to be normal is obviously some indication of how skewed our perspective tends to be.." - Douglas Adams

ambargh's easy agar

Edited by ambargh (05/18/10 09:16 PM)

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Offlineevildee125
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Re: CAn i use jello as agar? [Re: ambargh]
    #12588753 - 05/18/10 09:17 PM (14 years, 7 months ago)

ive used gelatine before... it sorta works.. it does alright for a bit until it liquifies


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