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OfflineNewbie2626
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Registered: 05/14/10
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Got an idea for a LC Tek, let me know what u guys think?
    #12578961 - 05/17/10 01:24 PM (14 years, 8 months ago)

Hey guys. I'm a new grower that's got an idea to produce LC. Rather then opening the jars with LC to drop in a section of agar into the jar or using a spoor syringe directly to the LC i'm going to try a different Tek and see how it goes.

Take small 250ml jars and either get lids with self healing injection ports and put in 5-10cc of prepared PDA (Potato dextrose agar) in the bottom of the jars. Mix in a little more water into the PDA To allow for sterilization In the PC. Put the lids on allowing for gas exchange and cover the top with foil. Pressure cook at 15 psi for 20 min. Leave to cool down but remove the jars from the PC before the agar solidifies. Close the lids on tight without removing the foil and place them on a flat surface.

Let the agar solidify and leave for 3 or 4 days to see if there's any contams on the agar.

Now use a sterilized syringe and take out the plunger, Stuff the syringe with pillow casing that's most with solution of water and hydrogen peroxide (1 part peroxide and 1o parts water). Do this in a sterile environment, in a glove box or in front of a flow hood. Flame the needle and after wiping the injection port with alcohol inject into  self healing port on the lid allowing air into jar to return the pressure to normal air pressure.

Now take either a Syringe with LC made from your favorite isolate or clone and inject into the jar 1 or 2 drops of LC into the jar onto the agar. Now incubate in a dark place for till the agar is colonized.

Inject with LC prepared with honey or malt/dextrose after flaming the tip. Fill the jar with LC till its about half or 3/4 full. Incubate again in warm dark place or incubator for 3 to 4 days agitating it every day 2 or 3 times a day and u should have LC ready for use or store in the fridge for future use.

Any thoughts on this guys???

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