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Offlinewedder
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Crazy Idea, would it work?
    #12578457 - 05/17/10 11:46 AM (14 years, 8 months ago)

I have a crazy idea that might just be dumb enough to work.
I have a few jars which are contaminated with a greyish green mold growing. Now, I was wondering if it would be possible to inject either H2O2 Bleach or Alcohol into the jar where the contaminated mold is growing. The H2O2 or whatever would kill the contaminant and everything else in the small area it was injected. Then (I don't know if this would be necessary) I could stick the syringe back into the jar and attempt to suck up all the excess H202.

If I were to try this, which solution would be best for killing the contaminated mold.

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Offlinewedder
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Re: Crazy Idea, would it work? [Re: wedder]
    #12578728 - 05/17/10 12:43 PM (14 years, 8 months ago)

Well I did it anyways. I used H2O2 and injected it into 4 different contaminated jars. I hope it works out, only time can tell.

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Offlinesolumvita
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Re: Crazy Idea, would it work? [Re: wedder]
    #12585006 - 05/18/10 11:10 AM (14 years, 7 months ago)

let us know, i suspect it would not really work, but you never know :grin:


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InvisibleFungal growth
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Re: Crazy Idea, would it work? [Re: solumvita]
    #12585027 - 05/18/10 11:16 AM (14 years, 7 months ago)

and be sure not to open them in your work area.

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OfflineBrennus
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Re: Crazy Idea, would it work? [Re: wedder]
    #12586583 - 05/18/10 03:34 PM (14 years, 7 months ago)

Quote:

wedder said:
I have a crazy idea that might just be dumb enough to work.
I have a few jars which are contaminated with a greyish green mold growing. Now, I was wondering if it would be possible to inject either H2O2 Bleach or Alcohol into the jar where the contaminated mold is growing. The H2O2 or whatever would kill the contaminant and everything else in the small area it was injected. Then (I don't know if this would be necessary) I could stick the syringe back into the jar and attempt to suck up all the excess H202.

If I were to try this, which solution would be best for killing the contaminated mold.




The rest of the mycelium that's visible might simply be mold mycelium that hasn't begun sporulating yet. This is a waste of time - dump out the jar and start over.

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OfflineSomeGuy
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Re: Crazy Idea, would it work? [Re: Brennus]
    #12588362 - 05/18/10 08:16 PM (14 years, 7 months ago)

I have actually had some success with this method. I injected 1cc of 70% isopropl into green mold every other day until it was otherwise colonized. Then I birthed it, at and cut that area off, and sprayed it (the wound) with disinfectant. I got 4 flushes. peroxide won't kill it. Neither will alcahol, but it will stop it from sporulating.

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Offlinewedder
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Re: Crazy Idea, would it work? [Re: SomeGuy]
    #12592376 - 05/19/10 01:55 PM (14 years, 7 months ago)

So it has been 2 days since I injected the H2O2 and so far nothing has happened. Here are some pictures showing the contamination. I know I don't have before pictures, but I promise you they looked just like these.



Next I plan on injecting bleach hoping it will have more of an effect. I don't know how much bleach I should use. I am thinking a 1:3 ratio should be good.

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OfflineSomeGuy
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Re: Crazy Idea, would it work? [Re: wedder]
    #12592412 - 05/19/10 02:01 PM (14 years, 7 months ago)

I looked at your jars....bad news, it appears you filter layer is way thick. I've never had a needle long enough to reach that substrate. I buy the biggest I can get. Therefor, I believe that you are growing 100% pure contamination

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InvisibleBoozie
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Re: Crazy Idea, would it work? [Re: wedder]
    #12592984 - 05/19/10 03:44 PM (14 years, 7 months ago)

You are wasting your time. There is no point in trying to save a few jars that are obviously quite heavily contaminated.
Just trash the jars and start fresh.

Is that a pink-ish shade in the 2nd pic, or just from the picture itself?


--------------------
"After silence, that which comes nearest to expressing the inexpressible is music." - Aldous Huxley


   

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Offlinewedder
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Re: Crazy Idea, would it work? [Re: SomeGuy]
    #12600108 - 05/20/10 06:50 PM (14 years, 7 months ago)

Quote:

paducahovoids said:
I looked at your jars....bad news, it appears you filter layer is way thick. I've never had a needle long enough to reach that substrate. I buy the biggest I can get. Therefor, I believe that you are growing 100% pure contamination



My filter layer is definitely not too thick. The tip of my syringe needle reaches about a half inch below the dry verm. I am not growing just contamination any noob can look at anyone of my jars and obviously see that mycelium is growing.

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Offlinewedder
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Re: Crazy Idea, would it work? [Re: wedder]
    #12600127 - 05/20/10 06:54 PM (14 years, 7 months ago)

I also don't care if you think I am wasting my time for it's my time and I can do what I want with it..

And yea, there is a pinkish shade it's not just from the picture. I have not Idea what it could be.

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Offlinewedder
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Re: Crazy Idea, would it work? [Re: wedder]
    #12600164 - 05/20/10 07:00 PM (14 years, 7 months ago)

So it has been one day since I injected the bleach.
This is the same contam spot as in the first picture. If you asked me I would say it looks like it is going away.


I took a picture of the second contam spot but the picture didn't come out good and I am too lazy to take another one. But it is also looking better.
I guess I will keep injecting bleach. At this point I don't care if I kill the rest of my mycelium in the process I just want the contams dead.

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OfflineSomeGuy
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Re: Crazy Idea, would it work? [Re: wedder]
    #12600204 - 05/20/10 07:06 PM (14 years, 7 months ago)

Quote:

wedder said:
Quote:

paducahovoids said:
I looked at your jars....bad news, it appears you filter layer is way thick. I've never had a needle long enough to reach that substrate. I buy the biggest I can get. Therefor, I believe that you are growing 100% pure contamination



My filter layer is definitely not too thick. The tip of my syringe needle reaches about a half inch below the dry verm. I am not growing just contamination any noob can look at anyone of my jars and obviously see that mycelium is growing.



This is my 18 guage bovine syringe beside a jar. Now look at the line on your jar. In any event you could have got a lot more substrate in that jar. Mine are filled to the bottom of the ring

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