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InvisibleMrPinkFloyd


Registered: 07/13/09
Posts: 683
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Is a needle necessary for agar inoculation?
    #12574715 - 05/16/10 06:06 PM (14 years, 8 months ago)

I have a flowhood, and a syringe with some spores I want out, onto agar.  Said syringe didn't come with an attached needle, just a cap.  Is it cool to remove the cap, in front of the flowhood no doubt, drop a drop on agar, then just cap it up.  My instincts say yes...What do you guys think?

I do have a bunch of used needles, used only once, still attached to vendor syringes I got from a buddy.  I'm not too sure how to go about just sterilizing these needles, then safely, sterilely, attaching them to the syringe I want to use....but if they aren't needed, like I'm thinking, it's a moot point.

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InvisibleShea25
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Registered: 01/27/09
Posts: 7,772
Loc: Westcoast Canada Flag
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Re: Is a needle necessary for agar inoculation? [Re: MrPinkFloyd]
    #12574727 - 05/16/10 06:08 PM (14 years, 8 months ago)

Quote:

MrPinkFloyd said:
I have a flowhood, and a syringe with some spores I want out, onto agar.  Said syringe didn't come with an attached needle, just a cap.  Is it cool to remove the cap, in front of the flowhood no doubt, drop a drop on agar, then just cap it up.  My instincts say yes...What do you guys think?

I do have a bunch of used needles, used only once, still attached to vendor syringes I got from a buddy.  I'm not too sure how to go about just sterilizing these needles, then safely, sterilely, attaching them to the syringe I want to use....but if they aren't needed, like I'm thinking, it's a moot point.





yes that will work or you can scrape spores on agar too

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InvisibleMrPinkFloyd


Registered: 07/13/09
Posts: 683
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Re: Is a needle necessary for agar inoculation? [Re: Shea25]
    #12574754 - 05/16/10 06:12 PM (14 years, 8 months ago)

Yea, I've done that a time or two, but never spores without a needle onto agar.

Thanks bub.

:thumbup:

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InvisibleShea25
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Registered: 01/27/09
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Re: Is a needle necessary for agar inoculation? [Re: MrPinkFloyd]
    #12574765 - 05/16/10 06:13 PM (14 years, 8 months ago)

This is how you sterilize a syringe and needle

http://www.shroomery.org/forums/showflat.php/Number/12098357#12098357

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OfflinehamloafM
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Registered: 12/23/09
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Re: Is a needle necessary for agar inoculation? [Re: Shea25]
    #12574883 - 05/16/10 06:34 PM (14 years, 8 months ago)

been there, done that. It worked.



Angle everything into your flowhood's sterile air flow, right up on the filter.
Be sure to spray the surface of your filter down with lysol, like you are doing a bubble fill-in, before you turn it on.
Let hood run for an hour to scrub air in your lab.
These items do not hurt to use while doing syringe/spore work.













IME. A better way to inoculate agar is with an inoculating loop and a spore print.

Edited by LiquidMyce (05/16/10 06:37 PM)

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InvisibleMrPinkFloyd


Registered: 07/13/09
Posts: 683
Loc: United States Flag
Re: Is a needle necessary for agar inoculation? [Re: hamloaf]
    #12575052 - 05/16/10 07:11 PM (14 years, 8 months ago)

Yea, I've had lots of success from a spore print, with a loop, but, this APE is in a syringe, so you know, gotta do what ya gotta do!

I wasn't sure if boiling the syringe/needle was enough to get in the part of the syringe where the needle locks into place, and if that would then contaminate the whole sha'bang.  I had the paranoia.

Thanks guys!

:peace:

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