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Offlinewhitedevil1
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Registered: 03/31/10
Posts: 262
Loc: eastern wa
Last seen: 9 months, 23 days
my first try is it contaminated
    #12564742 - 05/14/10 10:15 PM (14 years, 8 months ago)

I decided to give a small grow a try using BRF.
They were inoculated about4 days ago with a spore syringe and just noticed growth today but appears not has white has I expected. Only shows right were inoculated, and all jars look the same



So I let them keep going and while three of the jars just have big green spots, the other ones grew white over the green and appear to be spreading through the substrate, I will continue to let them grow just to see.

Edited by whitedevil1 (05/18/10 05:14 PM)

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OfflineYaYoKush
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Re: my first try is it contaminated [Re: whitedevil1]
    #12564752 - 05/14/10 10:18 PM (14 years, 8 months ago)

Doesn't look good kinda looks green.


--------------------
are we wizards? lol
...we do deal in various magics

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Offlinemadi
Hullu Mykologi
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Registered: 01/27/09
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Re: my first try is it contaminated [Re: YaYoKush]
    #12564778 - 05/14/10 10:24 PM (14 years, 8 months ago)

You need to start over, better luck next time!

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OfflineYaYoKush
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Re: my first try is it contaminated [Re: madi]
    #12564788 - 05/14/10 10:28 PM (14 years, 8 months ago)

Walk us through your sterile procedure maybe we can help you out.

Example:Did you PC or Steam?


--------------------
are we wizards? lol
...we do deal in various magics

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Offlinewhitedevil1
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Registered: 03/31/10
Posts: 262
Loc: eastern wa
Last seen: 9 months, 23 days
Re: my first try is it contaminated [Re: YaYoKush]
    #12564828 - 05/14/10 10:37 PM (14 years, 8 months ago)

I didn't have a pc so I steamed for 90 min on rolling boil.
The jars did sit for about 5 days but had electrical tape ever tops.
I have three other jars that were inoculated 1 day later with oysters that show no sign of any growth at all, good or bad.
I didn't really even try to be super sterile for the oysters(took spore print and made honey water lc, used a syringe to draw liquid and shoot in jars sitting at my dining room table).

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InvisibleAlienscience
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Registered: 07/19/09
Posts: 1,258
Re: my first try is it contaminated [Re: YaYoKush]
    #12564832 - 05/14/10 10:37 PM (14 years, 8 months ago)

Did you boil the substrate before sterilization.  This helps keep the moisture content perfect when jarred and also helps tremendously lower the chance of contam.

EDIT: Buy some store sold H poo and a bale of hay.  Soak the hay and poo in very warm water for a few hours and then let dry until slightly wet.  Place contammed cake in a trash bag along with hay and poo until entire bag is colonized.  Contam or not.  Wear a mask when opening it though, as mold spores will probably be all up in yo grill.  Lastly.  Find a nice shady, safe, and quiet spot outside and bury the entire contents of the bag under an inch or so of soil mixed in with leaves and forest debris.  I once read somewhere that nature has it's own way of working out contams naturally.  Also, I'm positive you don't want all of your hard work to go to waste. You might as well put it to some good use; because I know your going to try again.  Good luck.

Edited by Alienscience (05/14/10 10:44 PM)

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Offlinewhitedevil1
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Registered: 03/31/10
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Re: my first try is it contaminated [Re: Alienscience]
    #12564852 - 05/14/10 10:43 PM (14 years, 8 months ago)

Boil the substrate?
Its a brown rice flour vermiculite mix that I did heat in the oven and allow to cool has a extra step due to the lack of a pressure cooker.
Then I made my mix and filled the jars to let steam. with a half inch of dry verm on top and wrapped in a few layers of tin foil to keep water out during steaming.

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Offlineiamu
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Registered: 04/12/09
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Loc: Ireland
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Re: my first try is it contaminated [Re: Alienscience]
    #12564871 - 05/14/10 10:46 PM (14 years, 8 months ago)

? i dont see any green


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InvisibleAlienscience
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Re: my first try is it contaminated [Re: whitedevil1]
    #12564875 - 05/14/10 10:47 PM (14 years, 8 months ago)

Boiling a sub, specifically grains, helps release many of the endospores before sterilization.  A flour and verm mix might not work so well though.

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InvisibleAlienscience
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Re: my first try is it contaminated [Re: Alienscience]
    #12564892 - 05/14/10 10:50 PM (14 years, 8 months ago)

I think I see spider web.

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OfflineStampede
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Registered: 05/14/02
Posts: 430
Loc: PNW
Last seen: 3 months, 30 days
Re: my first try is it contaminated [Re: Alienscience]
    #12564910 - 05/14/10 10:53 PM (14 years, 8 months ago)

Quote:

Alienscience said:
Boiling a sub, specifically grains, helps release many of the endospores before sterilization.  A flour and verm mix might not work so well though.




Dude why do you keep talking about boiling the Substract .. he's doing BRF Cakes not grains.

Hey buddy ..sometimes till the mycelium gets stronger it can look kinda off white to start off with.  Give it a few more days. If it's trich it'll turn real green .. if it's just the mycelium it will become whiter.


--------------------
Stampede                     
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Offlinewhitedevil1
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Registered: 03/31/10
Posts: 262
Loc: eastern wa
Last seen: 9 months, 23 days
Re: my first try is it contaminated [Re: Stampede]
    #12564935 - 05/14/10 10:58 PM (14 years, 8 months ago)

Thanks for clearing up the "Boiling" conversation, I was trying to explain it was brf. I separated them and will watch over the next week.
I could understand losing all the jars or just a couple that got contamed but only the ones I inoculated that night with the B+ look like this. either it was something that night or that syringe. Or I get lucky like you said and it whitens up

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Offlineiamu
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Registered: 04/12/09
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Re: my first try is it contaminated [Re: whitedevil1]
    #12565005 - 05/14/10 11:10 PM (14 years, 8 months ago)

chances are not every one of your jars got cobweb mold. its probably mycelium


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OfflineBreakfast Crew
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Re: my first try is it contaminated [Re: iamu]
    #12565179 - 05/14/10 11:46 PM (14 years, 8 months ago)

How can anyone determine anything from that picture. After 1 week since injection you should see a healthy white spot.

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InvisibleAlienscience
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Posts: 1,258
Re: my first try is it contaminated [Re: Stampede]
    #12565244 - 05/15/10 12:05 AM (14 years, 8 months ago)

Quote:

Stampede said:
Quote:

Alienscience said:
Boiling a sub, specifically grains, helps release many of the endospores before sterilization.  A flour and verm mix might not work so well though.




Dude why do you keep talking about boiling the Substract .. he's doing BRF Cakes not grains.

Hey buddy ..sometimes till the mycelium gets stronger it can look kinda off white to start off with.  Give it a few more days. If it's trich it'll turn real green .. if it's just the mycelium it will become whiter.




"A flour and verm mix might not work so well though." dude. Besides, he doesn't want butt sex moron, I was only giving help.  Cause pally, he's contammed. So quit saying he aint.

Got you good, fuckkker.



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Offlinemastagreen
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Registered: 04/17/09
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Re: my first try is it contaminated [Re: Alienscience]
    #12565284 - 05/15/10 12:17 AM (14 years, 8 months ago)

Green.

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Offlineph34r
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Registered: 03/25/10
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Last seen: 12 years, 2 months
Re: my first try is it contaminated [Re: mastagreen]
    #12565441 - 05/15/10 01:03 AM (14 years, 8 months ago)

92% certain that's mold


--------------------
"Yeah well, you know...that's just like...your opinion, man." - The Dude

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OfflineStampede
Vet. Shroomerite!
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Registered: 05/14/02
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Loc: PNW
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Re: my first try is it contaminated [Re: Alienscience]
    #12567074 - 05/15/10 12:06 PM (14 years, 8 months ago)

Quote:

Alienscience said:
Quote:

Stampede said:
Quote:

Alienscience said:
Boiling a sub, specifically grains, helps release many of the endospores before sterilization.  A flour and verm mix might not work so well though.




Dude why do you keep talking about boiling the Substract .. he's doing BRF Cakes not grains.

Hey buddy ..sometimes till the mycelium gets stronger it can look kinda off white to start off with.  Give it a few more days. If it's trich it'll turn real green .. if it's just the mycelium it will become whiter.




"A flour and verm mix might not work so well though." dude. Besides, he doesn't want butt sex moron, I was only giving help.  Cause pally, he's contammed. So quit saying he aint.

Got you good, fuckkker.

quote]

What the fuck you talkin about dude .. your reply had nothing to do with what he had going on .. have a little mental stability and actually read what he had going on. It didn't even have anything to do with his situation or question.  DUMBASS.  And I never said he wasn't contaminated told him to hold off another day or two to be for sure. Cause if you don't get your spore solution all the way to the side it can give the brf an off color till it colinizes toward the direction of the glass.


--------------------
Stampede                     
Hunting the Hills and Pastures

FAPA - Member Since  5-18-02

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OfflineDharmatripper
Deceased

Registered: 09/05/09
Posts: 291
Loc: Over here
Last seen: 6 years, 5 months
Re: my first try is it contaminated [Re: YaYoKush]
    #12567115 - 05/15/10 12:14 PM (14 years, 8 months ago)

Yup:

Quote:

YaYoKush said:
Doesn't look good...




Give'em a couple days and see what you grow--enemy or ally.


--------------------
This is not me...

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Offlinebw86
Doesn't play well with others


Registered: 11/12/06
Posts: 5,976
Loc: 7b
Last seen: 6 hours, 11 minutes
Trusted Cultivator
Re: my first try is it contaminated [Re: Dharmatripper]
    #12567126 - 05/15/10 12:18 PM (14 years, 8 months ago)

try try and try again
im 5 years in with a flowhod and i still get contams every once in a while

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Shop: Original Sensible Seeds Bulk Cannabis Seeds   Bridgetown Botanicals CBD Concentrates   Left Coast Kratom Buy Kratom Extract   OlympusMyco.com We’re Not Chasing Unicorns—We’re Building Quality (Olympus Myco Grow Bags)   PhytoExtractum Kratom Powder for Sale   Unfolding Nature Unfolding Nature: Being in the Implicate Order   North Spore Bulk Substrate   Myyco.com Isolated Cubensis Liquid Culture For Sale   MagicBag.co Certified Organic All-In-One Grow Bags   Mushroom-Hut Liquid Cultures


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