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Offlineshroomiedoobie
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Registered: 03/25/10
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spores will germ on brf but not lc? wtf *DELETED*
    #12560872 - 05/14/10 09:17 AM (14 years, 8 months ago)

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Offlinetajmahal420
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Registered: 05/23/09
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Re: spores will germ on brf but not lc? wtf [Re: shroomiedoobie]
    #12561096 - 05/14/10 10:08 AM (14 years, 8 months ago)

Never heard of agave nectar for LCs, so I can't comment one way or the other about your medium.

I would think that injecting spores into an LC would be problematic, two compatible spores have to find each other, and I'd think that would be tough to do, considering all the liquid they're floating around in. I inoculated with agar wedges when I did LC, but no longer.  LC sucks IMO.

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Offlineleggomygreggo
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Re: spores will germ on brf but not lc? wtf [Re: tajmahal420]
    #12564664 - 05/14/10 09:51 PM (14 years, 8 months ago)

Go with Grain LC. So much easier. I have no success with LC yet but making little half pints of wbs and letting them grow and turning them into lc is very easy. First of all you know the LC is good and its just plain easier.

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OfflineSpongiform
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Re: spores will germ on brf but not lc? wtf [Re: tajmahal420]
    #12565706 - 05/15/10 02:23 AM (14 years, 8 months ago)

Quote:

tajmahal420 said:
Never heard of agave nectar for LCs, so I can't comment one way or the other about your medium.

I would think that injecting spores into an LC would be problematic, two compatible spores have to find each other, and I'd think that would be tough to do, considering all the liquid they're floating around in. I inoculated with agar wedges when I did LC, but no longer.  LC sucks IMO.




When I inoculate LC's I use a very small amount of spore solution, around 1/4cc.  After inoculating I do not shake or swirl for about 3 days to give the spores time to germinate and do their thing.  I started 4 LC's about a week ago and 3 out of 4 are doing great.  The 4th one is from a questionable source though. Friend of a friend made it a couple years ago...

Here's one of them at day 9 or so.



I think the trick with LC is to just do it right.  Try to cut corners or use iffy filters and your chance of failure goes way up.

Sounds like the issue might be your spore source since you're having trouble across the board.


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