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Offlinep14y80y
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A story to remember?... Speechless.
    #12548498 - 05/12/10 12:38 PM (14 years, 8 months ago)

I can already imagine what types of comments that might be replied to this so in advance please be nice. I have grown maybe 8 times on different levels mostly all bulk, even the first one (60 jars). I guess this is a story for other noobs to maybe learn from and I have a few questions because it's not a complete failure.

Here are the facts:

Part 1    Part 2  - 5/13  re-run(50 lbs. of Rye)
75 lbs. of Rye Berries

11 half gallon
113 quart
30 half quart
---154 jars---
150 quarts

50 LC needles
64 Multi-spore cc's - 10 different strains all used before.

Incubated at 78-81F


Some of the lids are rather ghetto as about 50 jars are the OLDER style with the wire clasp. All lids have an injection port with mopar sealant and double layered breathable micro-pore tape. Because at least 100 of my lids are plastic I used a red tape that supports the heat and is very sticky and strong, around the jars to keep the lids on tights, the same with the metal lids that wouldn't correctly fit on the OLDER styled jars.

May-2 Rinse 4-5 times, Soaked for 24hours

May-3 Boiled for 10 minutes, starting at 10PM. Drained instantly after the boil (extended time because of the process of everything else - steps wise), I finally was able to move it all and arrange it to dry (picture) around 1AM. I let it dry from that point and started loading the jars around 3:30AM, went to sleep just before 7AM.



May-4 Started the stove first load about 4PM and cycled one after another until I couldn't stay awake any longer around 11:30PM. I shook the HOT jars just as they came out of the cookers. Big enough foil was over the lids as well.

May-5 Started the last of it about 7AM. Finally was done around 11AM. (Because of the time I had at the friend's, I made the mistake of taking the valve off each time, helping maybe make the problems I currently have).

May-6 Day 1 - After lugging everything there, used the stove for almost a full day, and set it all back up; I started to inject. I shook them all after injecting because I seen moisture and I used 2cc's of LC each 1 quart and .5 quart jar, 3cc's per .5 gallon jar. Then 1cc of multispore solution for the rest of the 64 jars. This took about 4 hours because of the glove box and room I had to work with. The funny thing as much as I was happy to be down with it at this point, I thought I could reap in the joy of what I felt was a concured struggle. Hahahahaha, yea right.







I have had a healthy LC for awhile and know that the syringes were safe because I have had healthy growth in the maybe 35 jars, 'current'. Those jars, all started fast even though I shook them up after injection. I have had to ditch about 25 jars from green mold within the last 4 days. Obviously the 64 MS don't have any growth yet. A good 35 LC jars do not have any growth at all.

Most of the fast growth jars appear to have a green little dot started.:frown:



Questions:

I have made a few mistakes that I know of, as I haven't grown in almost 2 years. One is I know I let the grains dry for a few hours because they still were "damp". Does anyone have a different idea/method for drying larger bulk than normal, other than the picture from what I have tried last? Not letting the pressure cooker cool before taking them out. Another issue is that I had to bring everything to a friend's house to pressure cook them, (which will be avoided for the second trial about a day of 50 lbs.)

What can I do to re-hydrate the already processed grains that have already been injected?

What are the negatives toward re-injecting a week later?

Why and what can be done with this type of problem in which (most) of the healthy ones are going through? Most of the ones I have thrown out and a few of the good ones have 1 green spot the size of a pens diameter. Does this help anyone understand the reason for my downhill? The X amount of good ones all were 3/4's colonized, then I shook them... I noticed in some that "famous" green smokey dust within the left over space within a few jars.





Besides don't do as much after the next 50 lbs. tomorrow, what other input could I use?

I hope regardless in the end I learn and will be more prepared in ever angle for the next time. Though I still have about 50lbs. left that I have soaking now and going to hopefully correct and adjust so this batch is better off. From what I have given-said, my problems are from the grains I assume and I believe "most" of it is because of the moisture content. I have 8 bags I am going to be using that I have left along with the extra jars that are not currently in use from this incomplete project. Any questions or suggestions would be helpful.





Where do I find the biggest bandage for this hurt? :sad:

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OfflineBrennus
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Re: A story to remember?... Speechless. [Re: p14y80y]
    #12548671 - 05/12/10 01:12 PM (14 years, 8 months ago)

Are you trying to figure out your vector for contamination?

You probably have dirty liquid culture. Sugar water LCs are not recommended, specifically for this reason. You probably would've had better luck with myceliated water.

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Offlinep14y80y
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Re: A story to remember?... Speechless. [Re: Brennus]
    #12548835 - 05/12/10 01:38 PM (14 years, 8 months ago)

I guess the main things deals with the contamination. What are the reasons for a jar to start growing healthy and then all of a sudden turn the other way. Also is it possible to re-hydrate with more liquid culture or sterile water?

I did use karo light for the LC, but if it was contaminated then why would mycelium form?

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OfflineBrennus
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Re: A story to remember?... Speechless. [Re: p14y80y]
    #12548872 - 05/12/10 01:44 PM (14 years, 8 months ago)

When you shoot spores into sugar water to make LC, there's no way to tell if your culture is clean before you knock up jars. Mold spores could've landed on your needle, in your media container, etc. etc.

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Offlinep14y80y
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Re: A story to remember?... Speechless. [Re: Brennus]
    #12548914 - 05/12/10 01:50 PM (14 years, 8 months ago)

I understand what you're just the thing is there is mycelium growth? If it was contaminated how could mycelium form? I have never read or heard that but that would suck because that is all I have left for the rest 50 lbs. soaking today.

I thought if mycelium formed then the culture was healthy. If something happened after growth grew then a different factor made the extra mold grow. Basically that a contaminated culture couldn't grow white healthy myceluim.

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OfflineBrennus
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Re: A story to remember?... Speechless. [Re: p14y80y]
    #12551780 - 05/12/10 08:45 PM (14 years, 8 months ago)

Quote:

p14y80y said:
I understand what you're just the thing is there is mycelium growth? If it was contaminated how could mycelium form? I have never read or heard that but that would suck because that is all I have left for the rest 50 lbs. soaking today.

I thought if mycelium formed then the culture was healthy. If something happened after growth grew then a different factor made the extra mold grow. Basically that a contaminated culture couldn't grow white healthy myceluim.




There's no way to tell if your LC is clean or dirty simply by looking at it. You could have an LC full of seemingly-healthy growth that only gives you jars chock full of green hate.

Mycelium-laden water is a different story. Since you already know your grain spawn is clean and viable, you know it'll work. Check out the "Grain LC" link in my signature.

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Offlinep14y80y
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Re: A story to remember?... Speechless. [Re: Brennus]
    #12556622 - 05/13/10 05:10 PM (14 years, 8 months ago)

Next time if most of this goes in the direction it is headed in for the first run I will. I saved your page as a reminder though. :thumbup:

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OfflineRogerRabbitM
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Re: A story to remember?... Speechless. [Re: p14y80y]
    #12556973 - 05/13/10 06:08 PM (14 years, 8 months ago)

Quote:

p14y80y said:
I understand what you're just the thing is there is mycelium growth? If it was contaminated how could mycelium form? I have never read or heard that but that would suck because that is all I have left for the rest 50 lbs. soaking today.

I thought if mycelium formed then the culture was healthy. If something happened after growth grew then a different factor made the extra mold grow. Basically that a contaminated culture couldn't grow white healthy myceluim.




Mold is mycelium too, and you can't tell it in the early stages from mushroom mycelium.  There's no saving those 'good' jars with green mold in them.  Toss it all out and start over from scratch.  Watch the grain prep video if you haven't already.
RR


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semper in excretia sumus solim profundum variat

"I've never had a failed experiment.  I've only discovered 10,000 methods which do not work."
Thomas Edison

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Offlinep14y80y
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Re: A story to remember?... Speechless. [Re: RogerRabbit]
    #12557292 - 05/13/10 07:03 PM (14 years, 8 months ago)

Yea I actually bought your dvd but I guess I simply messed it up because I know I cut a few corners and tried to do too much and had a very small time line. I have a second batch 50lbs. of Rye that is cooling now so maybe I still be on target for what I hoped. Thanks.

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Offlinep14y80y
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Re: A story to remember?... Speechless. [Re: p14y80y]
    #12557339 - 05/13/10 07:08 PM (14 years, 8 months ago)

Hey Roger, what would you suggest or even just as a quick one time use to replace the old type of wire/glass jar lids? What I have done is really at the low end of a cob-job and would like to think of something (material) else to use other than tin foil and tape with GE/Inject holes.

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OfflineRogerRabbitM
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Re: A story to remember?... Speechless. [Re: p14y80y]
    #12561146 - 05/14/10 10:28 AM (14 years, 8 months ago)

I would suggest you use mason jars with two piece lids as shown in the video.  With grains, the DVD shows four methods of filtering.  I prefer synthetic filter disks.
RR


--------------------
Download Let's Grow Mushrooms



semper in excretia sumus solim profundum variat

"I've never had a failed experiment.  I've only discovered 10,000 methods which do not work."
Thomas Edison

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Offlinep14y80y
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Re: A story to remember?... Speechless. [Re: RogerRabbit]
    #12562250 - 05/14/10 02:29 PM (14 years, 8 months ago)

I know that most of grains are way too dry from how I prepared everything and on the side of that I soaked the jars in bleach for a good 4 hours and there was definitely green in the jars from last time. So I'm going to have to say that it makes sense why this batch is going down hill and fast. Though the second batch was done more on target from your video. It is hard to do a larger amount with little control over the place it's being done in and independently. For now on I will have better planning and do things in parts.

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Offlinep14y80y
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Re: A story to remember?... Speechless. [Re: p14y80y]
    #12580050 - 05/17/10 04:07 PM (14 years, 8 months ago)

It was the LC. I am only going to do a grain to grain for now on and use only the Mycelium-laden water. A great deal of work for a lesson well learn. I have used the LC before hand though, something must have happen in between the time frame.

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