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hifana
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Registered: 05/12/10
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How do i go about pasturizing my bulk wbs?
#12548342 - 05/12/10 11:59 AM (14 years, 8 months ago) |
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Ive just hydrated and dried my bulk wbs, now Im wondering how to pasteurize it. Ive got a pressure cooker, but I dont know what to put the batch of wbs in so it dosnt get wet when I pressure cook it.
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sewy
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Registered: 08/30/09
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Re: How do i go about pasturizing my bulk wbs? [Re: hifana]
#12548352 - 05/12/10 12:02 PM (14 years, 8 months ago) |
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hifana
Stranger

Registered: 05/12/10
Posts: 34
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Re: How do i go about pasturizing my bulk wbs? [Re: sewy]
#12548393 - 05/12/10 12:11 PM (14 years, 8 months ago) |
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Ive got a ton of jars but the jids all have custom tyvek/silocone filters which could have been contamed when all the spawn in them stalled. Ive since half effortly emptied the jars of all dead substrate and left them out side. Theres no visual mold in the jars or in the filters, but something cased them (all 9) to stall soIm a bit concerned about using them again. If I put the lids in the oven will they be alright, or is pressure cooking enough to make sure all the potential contams die?
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sewy
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Re: How do i go about pasturizing my bulk wbs? [Re: hifana]
#12548402 - 05/12/10 12:14 PM (14 years, 8 months ago) |
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Pressure cooking will re sterilize your lids, just make sure theres no old grains still stuck to em. also get rid of the tyvek and use polyfil
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Giggle_Grower
A lil less noob each day



Registered: 04/07/10
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Re: How do i go about pasturizing my bulk wbs? [Re: hifana]
#12548407 - 05/12/10 12:14 PM (14 years, 8 months ago) |
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PC should kill off everything if done properly
-------------------- I'm always interested in trades.
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Noobs! The best tool here is up top to the right. Don't forget about it. Just type your question in!
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prismism



Registered: 05/11/09
Posts: 5,570
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Re: How do i go about pasturizing my bulk wbs? [Re: hifana]
#12548427 - 05/12/10 12:20 PM (14 years, 8 months ago) |
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Quote:
hifana said: Ive got a ton of jars but the jids all have custom tyvek/silocone filters which could have been contamed when all the spawn in them stalled. Ive since half effortly emptied the jars of all dead substrate and left them out side. Theres no visual mold in the jars or in the filters, but something cased them (all 9) to stall soIm a bit concerned about using them again. If I put the lids in the oven will they be alright, or is pressure cooking enough to make sure all the potential contams die?
I'm confused. Why are you pasteurizing wild bird seed? Are you wanting to use it as a bulk substrate material? Wbs needs to be sterilized.
-------------------- ephemeral anomalous
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hifana
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Re: How do i go about pasturizing my bulk wbs? [Re: Giggle_Grower]
#12548431 - 05/12/10 12:22 PM (14 years, 8 months ago) |
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Folyfill? That fluffy stuff in pillows? Also, my pc dosnt have a gauge on it, dont know if that could be a problem.
Yh Im a noob sry. Im gusing pressure cooking will sterilize it..
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sewy
Noobie


Registered: 08/30/09
Posts: 38
Last seen: 10 years, 3 months
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Re: How do i go about pasturizing my bulk wbs? [Re: hifana]
#12548459 - 05/12/10 12:28 PM (14 years, 8 months ago) |
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Quote:
hifana said: Folyfill? That fluffy stuff in pillows? Also, my pc dosnt have a gauge on it, dont know if that could be a problem.
Yh Im a noob sry. Im gusing pressure cooking will sterilize it..
Yes the fluffy stuff in your pillow, look at that tek I gave you again. No guage? Thats a problem if you don't know what psi your pc is at.
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tekramrepus


Registered: 02/20/02
Posts: 2,253
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Re: How do i go about pasturizing my bulk wbs? [Re: sewy]
#12548986 - 05/12/10 02:02 PM (14 years, 8 months ago) |
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You need to read more, to be honest.
The point of using WBS is to SPAWN to bulk, WBS itself is NOT bulk.
Grains, such as popcorn, wbs, rye - are used by themselves and cased w/ a casing layer, or spawned to bulk substrate, such as wheat straw, horse poo, or coco coir.
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kechlesurf
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Re: How do i go about pasturizing my bulk wbs? [Re: hifana]
#12549018 - 05/12/10 02:07 PM (14 years, 8 months ago) |
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Quote:
hifana said: Folyfill? That fluffy stuff in pillows? Also, my pc dosnt have a gauge on it, dont know if that could be a problem.
Yh Im a noob sry. Im gusing pressure cooking will sterilize it..
I have been using WBS for years and use one layer of tyvek per jar. You can use more if you like but i do not have any problems with it. With polyfill, you need to put a hole in the lid, but wiht tyvek, just use the ring and discard the lid. You do not need a gauge for your pressure cooker, they are always nice but as long as yours has a rocker on the lid, you will be fine.
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punkrocker292004
i am a liar



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Re: How do i go about pasturizing my bulk wbs? [Re: kechlesurf]
#12549025 - 05/12/10 02:09 PM (14 years, 8 months ago) |
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just put foil over your lids to kep the wet out
-------------------- EVERYTHING YOU NEED TO KNOW
on a long enough timeline the survival rate for everyone drops to zero-tyler durden
watch me
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punkrocker292004
i am a liar



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Re: How do i go about pasturizing my bulk wbs? [Re: kechlesurf]
#12549042 - 05/12/10 02:13 PM (14 years, 8 months ago) |
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Quote:
kechlesurf said:
Quote:
hifana said: Folyfill? That fluffy stuff in pillows? Also, my pc dosnt have a gauge on it, dont know if that could be a problem.
Yh Im a noob sry. Im gusing pressure cooking will sterilize it..
I have been using WBS for years and use one layer of tyvek per jar. You can use more if you like but i do not have any problems with it. With polyfill, you need to put a hole in the lid, but wiht tyvek, just use the ring and discard the lid. You do not need a gauge for your pressure cooker, they are always nice but as long as yours has a rocker on the lid, you will be fine.
when i use tyvek some times the jar lid cuts it and its fucked
-------------------- EVERYTHING YOU NEED TO KNOW
on a long enough timeline the survival rate for everyone drops to zero-tyler durden
watch me
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kechlesurf
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Registered: 11/30/07
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Quote:
punkrocker292004 said:
Quote:
kechlesurf said:
Quote:
hifana said: Folyfill? That fluffy stuff in pillows? Also, my pc dosnt have a gauge on it, dont know if that could be a problem.
Yh Im a noob sry. Im gusing pressure cooking will sterilize it..
I have been using WBS for years and use one layer of tyvek per jar. You can use more if you like but i do not have any problems with it. With polyfill, you need to put a hole in the lid, but wiht tyvek, just use the ring and discard the lid. You do not need a gauge for your pressure cooker, they are always nice but as long as yours has a rocker on the lid, you will be fine.
when i use tyvek some times the jar lid cuts it and its fucked
Dont use the lid, just the ring. Cut a square piece of tyvek, put it over the jar, and screw the ring on.
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punkrocker292004
i am a liar



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Re: How do i go about pasturizing my bulk wbs? [Re: kechlesurf]
#12549162 - 05/12/10 02:29 PM (14 years, 8 months ago) |
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that is what i do lol i think i screw um on too tight
-------------------- EVERYTHING YOU NEED TO KNOW
on a long enough timeline the survival rate for everyone drops to zero-tyler durden
watch me
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Barakanaten
Ama-gi




Registered: 04/14/10
Posts: 6,163
Loc: PNW Mycosphere( Blessed C...
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Re: How do i go about pasturizing my bulk wbs? [Re: hifana]
#12549676 - 05/12/10 04:03 PM (14 years, 8 months ago) |
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Read this whole thing & it will give you a better idea.
https://www.shroomery.org/forums/showflat.php/Number/9285870
Quote:
3. Preparing Bulk Substrate and Spawning
Here is the info on how to prepare different bulk substrates. There is a lot more info doing a quick search using the search engine.
IMPORTANT. Bulk substrates are PASTEURIZED not sterilized, as sterilization increases contamination rate dramatically.
Method 2 Grains and Bulk Growing
Quote:
Nibin said: Method 2 Grains and Bulk Growing
In this way of growing you first have to prepare grains with a pressure cooker as your growing medium and then decide if you fruit them directly or use them to make a bulk grow
The procedure is as follows
1. Prepare Grain and inoculate 2. Colonize 3. Prepare and spawn to your bulk substrate 4. Colonize 5. Case 6. Fruit
Basically you use your colonized grains as what is called spawn to introduce mushroom mycelium into a substrate that can be prepared easily in big quantities (hence the bulk part) by pasteurization not sterilization. This allows you to grow much larger amounts of mushrooms with not much more extra effort. Examples of bulk substrates are straw, horse manure or coco coir.
While spawn is normally colonized grain, you can actually also use broken up PF tek cakes, and if you have been patient enough to read all of this you have just discovered the way to grow in bulk without using a pressure cooker (as the pressure cooker is required to prepare the grains, not the bulk substrates)
As before, there are many teks written about how to do these procedures and I will just give you a few tips on each step.
1. Prepare grains
You can use different kinds of grain for this, but favourites are Rye, Wild Bird Seed and Corn.
Here you can find instructions on how to prepare the grains.
One of my favourites which isn't in the section above is the way RR prepares his rye
Quote:
My rye tek. I haven't posted this here in awhile.
Measure out your organic rye berries from a health food store, 1 cup for each quart jar you intend to make. Place them in a large pot. Rinse the heck out of them. Fill the pot with hot tap water, shake and swirl it around and pour it out. Do this three or four times until the water you pour out is clear. You'll be able to see when you have nice clean water to pour off instead of water filled with chaff and dirt. You want to now cover the rye berries with three times as much water as you have rye. Use half coffee(or less) and half plain water. In other words, if you have two inches of rye in the bottom of your pan, you should have six inches of water/coffee above that, for a total of 8 inches. Add one tablespoon of gypsum to the water and stir well. Leave this to sit for 12 to 24 hours.
After the soak, add water to nearly fill the kettle up, but leave room at the top for it to boil. Stir well and set the pot on the stove. Bring to a boil. Boil for ten minutes, then, WHILE BOILING, drain the contents through a very large colander. (spaghetti strainer) If you're making a large batch, you may need more than one colander. Tip the colander side to side to get the rye to drain as much of the water as you can. Then, shake the colander in order to 'toss' the grain. This will cause a lot of steam to rise from your rye. Do this a time or two, then let it sit for five minutes, then repeat. When all the moisture that will drip or evaporate from your rye has already done so, load your jars. The rye should look and feel dry to the touch when you load the jars. All the moisture you need is inside the grain. Fill jars no more than 2/3 full if they are to receive grain to grain transfers, or no more than 3/4 full if they are to be inoculated by spore syringe. Use a lid with a synthetic filter disk, polyfill, tyvek or similar. Cover with foil and PC the jars for at least 90 minutes at 15lbs. When the jars are cool, they're ready to inoculate.
2. Colonize the grain
Do not shake your grains just after injecting spores, wait until around 1/3 of the jar is covered in mycelium before shaking it.
Once you have colonized grain you can skip the next step and just case it and fruit it directly if you want to, but by using it as spawn you will increase your amount of substrate dramatically. Using the recommended 1:4 ratio will mean that 1 pint of grain spawn will become 5 pints of colonized bulk substrate (1 pint grain + 4 pints substrate) with just an additional weeks wait.
3. Preparing Bulk Substrate and Spawning
Here is the info on how to prepare different bulk substrates. There is a lot more info doing a quick search using the search engine.
IMPORTANT. Bulk substrates are PASTEURIZED not sterilized, as sterilization increases contamination rate dramatically.
A NOTE ON COIR: Coco Coir is processed coconut husk also used for plants. It was thought until a few years ago that is contained no nutrients and was a good casing (next section) material. Now we know that it actually does contain nutrients and is a good bulk substrate. So if you see any casing instructions using coir, try and avoid them.
I recommend you do not leave a layer of spawn on the top of the bulk substrate once you mix it all as if it dries out it can become a source of contamination.
4. Colonize bulk substrate
Here you will have to choose what setup you decide to go with.
The most common are trays which go into a normal fruiting chamber or into a Martha (plastic greenhouse) or a setup called a monotub. A quick search should give you loads of info on these setups.
Another good tip is to line the bottom of your container with a sheet of black plastic from a cut up rubbish/trash bag. This helps prevent side and bottom pinning as it sticks to the substrate as it shrinks.
5. Casing
To case or not to case, that is the question
Some substrates such as horse manure and coir will fruit well uncased, but all bulk substrates will benefit from a casing layer.
Here is the info on how to prepare a casing layer.
Another fantastic thread on casing is Hyphae's Pinning Strategy
6. Fruiting
Depending on your setup you will have different kinds of fruiting chamber. One important thing to remember is that pinning triggers as mentioned in the previous post that covers the PF tek still apply, but humidity should be around 90%.
This should help.
--------------------
New to cultivation? Have a Question? Because AMU We Q&A Know AMU Genetic Library Easy Felt  AMU  Official PNW Mushroom Hunting Season 2011"...Though in all else he may be as Saintly as St. Francis, in the pursuit of these ends he is more
Satanic than Satan. He will betray his nearest and dearest without the slightest twitch of flesh or spirit.
He is amoral."
-Definition of a mushroom hunter by ~Angelo Pellegrini
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Mad Hatter 2010



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Re: How do i go about pasturizing my bulk wbs? [Re: tekramrepus]
#12549693 - 05/12/10 04:06 PM (14 years, 8 months ago) |
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Quote:
supermarket said: You need to read more, to be honest.
The point of using WBS is to SPAWN to bulk, WBS itself is NOT bulk.
Grains, such as popcorn, wbs, rye - are used by themselves and cased w/ a casing layer, or spawned to bulk substrate, such as wheat straw, horse poo, or coco coir.
+1
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hifana
Stranger

Registered: 05/12/10
Posts: 34
Last seen: 13 years, 5 months
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Re: How do i go about pasturizing my bulk wbs? [Re: Mad Hatter 2010]
#12590975 - 05/19/10 09:16 AM (14 years, 7 months ago) |
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You guys are lifesavers. I got some straw from a local petshop to pasteurize (cheers guys), then looked at my jar..

With shrooms growin in the jar will it still b ok to crumble it into the straw? I also noticed several blue patches on the mycelium aswell, donno if its brusing or mold..
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Barakanaten
Ama-gi




Registered: 04/14/10
Posts: 6,163
Loc: PNW Mycosphere( Blessed C...
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Re: How do i go about pasturizing my bulk wbs? [Re: hifana]
#12591687 - 05/19/10 11:49 AM (14 years, 7 months ago) |
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The blue patches are because the mycelium is struggling
--------------------
New to cultivation? Have a Question? Because AMU We Q&A Know AMU Genetic Library Easy Felt  AMU  Official PNW Mushroom Hunting Season 2011"...Though in all else he may be as Saintly as St. Francis, in the pursuit of these ends he is more
Satanic than Satan. He will betray his nearest and dearest without the slightest twitch of flesh or spirit.
He is amoral."
-Definition of a mushroom hunter by ~Angelo Pellegrini
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