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Offlinewillytheweasal
Stranger
Registered: 01/19/03
Posts: 9
Last seen: 18 years, 18 days
Been 5 days..still no sign of colonization!!
    #1254737 - 01/28/03 08:51 AM (18 years, 1 month ago)

first of all this is my first attempt and i might not of done everything right, esp the PF substrate mixture. I have BRF/verm in there w/ distilled water. Boiled the jars for 1 hour and innoculated 12 hrs later.

one thing i noticed was that my substrate expanded after boiling and seem to be compacted in the jars w/ little space for air flow. could this have something to do w/ it? right now they're incubating in a rubbermaid with perlite for humidity, a heating pad. Should i start worrying now????


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OfflineBladeLSD
Squirrel Master

Registered: 08/25/00
Posts: 805
Last seen: 7 days, 22 hours
Re: Been 5 days..still no sign of colonization!! [Re: willytheweasal]
    #1254755 - 01/28/03 08:55 AM (18 years, 1 month ago)

Sounds like it might be water logged, possibly water sloshed into some of your jars during sterilization


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OfflineHamurabi
the babylonianleader..

Registered: 03/31/02
Posts: 2,418
Loc: Greece
Last seen: 1 year, 5 months
Re: Been 5 days..still no sign of colonization!! [Re: BladeLSD]
    #1254785 - 01/28/03 09:05 AM (18 years, 1 month ago)

don't do any full of panic moves. just wait! mushrooms should/will teach you to have patience


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Offlinewillytheweasal
Stranger
Registered: 01/19/03
Posts: 9
Last seen: 18 years, 18 days
Re: Been 5 days..still no sign of colonization!! [Re: willytheweasal]
    #1254865 - 01/28/03 09:33 AM (18 years, 1 month ago)

thank all...i'll wait and see (if i fucked up or not).


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Offlinelivingsublime
Stranger

Registered: 10/27/02
Posts: 555
Last seen: 18 years, 1 month
Re: Been 5 days..still no sign of colonization!! [Re: willytheweasal]
    #1254902 - 01/28/03 09:41 AM (18 years, 1 month ago)

Well in my myco-bag I diden't seee any growth for 10 days,but withen the last 4 days,the mycelium has doubled in size.
It usealy takes anywhere from 2 days to a month,10 days is probably the ave.


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OfflineBavet
Sensitive StonedRebel

Registered: 12/12/02
Posts: 383
Last seen: 9 years, 5 days
Re: Been 5 days..still no sign of colonization!! [Re: willytheweasal]
    #1254906 - 01/28/03 09:43 AM (18 years, 1 month ago)

Quote:

also, your incubator should NOT have perlite
in it.




Why not? I have it in my incubation chamber for my jars and I have no problems. I add water to the perlite now n then. Any water that may acumulate on the floor of my chamber I soak up with paper towel n disguard. I have zero contamination.


--------------------
"~Dream as if you'll live forever....live as if you'll die today~ James Dean"


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OfflineRobbyrob
Cubensis seeker

Registered: 12/27/02
Posts: 653
Loc: Earth
Last seen: 10 years, 9 months
Re: Been 5 days..still no sign of colonization!! [Re: willytheweasal]
    #1254908 - 01/28/03 09:44 AM (18 years, 1 month ago)

weasl take it from me man.. I have tried to see if there were any shorcuts and NOPE.. We have to learn to wait in this hobby.. I understand that you are dying to see this happen (as i am) but we gotta wait.. I just iculated on 1/26 so i am no where near to seeing anything,, Just bought a thrmo. today abd its only like 71 in my rubbermaid container.. I might have to get a heater as i want to be close to 78-80 deg.. maybe ill get one of those nice heat rocks..

Peace!


ps.. perlite will rise your humidity which as this point has no importance.. If yu need to have anything in there in a heater and thermometer for your readings..

the incubator link on annos page is nice for us newbies.. :grin: 


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Invisibledog
straw dog

Registered: 06/20/02
Posts: 2,790
Loc: Route 66
Re: Been 5 days..still no sign of colonization!! [Re: Bavet]
    #1254917 - 01/28/03 09:46 AM (18 years, 1 month ago)

Quote:

--------------------------------------------------------------------------------

also, your incubator should NOT have perlite
in it.


--------------------------------------------------------------------------------



Why not?




Because humidity is not required for incubation. It is a waste of time and perlite.


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Fascism (fash'izem) n. A governmental system marked by a centralized dictatorship, stringent socioeconomic controls, and often belligerent nationalism. see also: the Bush Administration.


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Offlinevatoloco
Puppet Hunter -DBK
Registered: 01/30/02
Posts: 7,653
Last seen: 16 years, 5 months
Post deleted by MOE THE MAD SCIENTIST [Re: dog]
    #1255009 - 01/28/03 10:16 AM (18 years, 1 month ago)



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InvisibleSixCee
keep rolling
Male

Registered: 06/12/02
Posts: 3,720
Loc: US, Chicago
Re: Been 5 days..still no sign of colonization!! [Re: vatoloco]
    #1255172 - 01/28/03 11:04 AM (18 years, 1 month ago)

I disagree with that. Though you can get away with small jars in incubation you need at least some air exchange for some bigger things. Try not making any holes in a quart jar and innoculate it. Growth will most likely halt.


--------------------
~~~~~~~~~~~~~~~~~~~~~~~~
-->The above statements may or not be true.
->Quote of the Moment :
"Yea. All bitches are whores who love sex." -Cubie
----> PMs checked daily.


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Offlinefugu
Pooh-Bah

Registered: 01/06/03
Posts: 2,223
Loc: istanbul
Last seen: 10 years, 7 months
Re: Been 5 days..still no sign of colonization!! [Re: SixCee]
    #1255278 - 01/28/03 11:37 AM (18 years, 1 month ago)

lots of times %90 humidity .drives me crazy. i ddnt believe my eyes how things can go that fast..but the most and deadly suggestion is close your jar lids tight .or use fresh foil all the time and dont meddle with them to much.if you want to look them ,take them out .


--------------------
mushroom culture history making ...Mr. Allan is the best .....


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Mushrooms, Mycology and Psychedelics >> Mushroom Cultivation

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