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Offlinefunksion
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Registered: 01/27/03
Posts: 12
Loc: virginny
Last seen: 14 years, 4 months
using spawn to.... well, spawn
    #1254448 - 01/28/03 07:14 AM (15 years, 3 months ago)

Let's say i placed a cake (millet) inside a spawn bag with unpasteurized Scotts 3n1.. let's say the compost colonized very nicely with no contams whatsoever.

If i were to take 90 percent of the spawned compost out of the bag and case it, then i placed more unpasteurized Scotts 3n1 into the spawn bag, is it feasible to believe that the 10 % left over would colonize the bag again? (granted, i must be very sterile in transferring....)

if this is true, then you could have a never ending supply of spawn w/o having to colonize 1/2 pints any longer.. correct? i hope so. i think i'll try either way, i just wanted some opionions. Thanks.


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Offlinevatoloco
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Registered: 01/30/02
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Post deleted by MOE THE MAD SCIENTIST [Re: funksion]
    #1254454 - 01/28/03 07:16 AM (15 years, 3 months ago)



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Offlinefunksion
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Registered: 01/27/03
Posts: 12
Loc: virginny
Last seen: 14 years, 4 months
Re: using spawn to.... well, spawn [Re: vatoloco]
    #1254467 - 01/28/03 07:20 AM (15 years, 3 months ago)

i appreciate that vato! encouragement goes a long way!!

these spawn bags are so damn big, next time i'm gonna throw a cake in there with as much compost as i can possibly fit..

btw, do you find that this is more logical (in terms of colonizing times) than spawning the myc in the casing pan/rubbermaid? b/c it took less than a week to colonize the bag.


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OfflineJssMthrFcknChrst
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Registered: 10/12/02
Posts: 446
Loc: Vatican City
Last seen: 13 years, 8 months
Re: using spawn to.... well, spawn [Re: funksion]
    #1254480 - 01/28/03 07:25 AM (15 years, 3 months ago)

Each time you respawn, your strain gets a little weaker. Eventually, you won't be able to take it any further. G2G transfers work the same way. It is best to keep using as young a generation as possible.


--------------------



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OfflineRaadt
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Registered: 06/07/02
Posts: 2,104
Loc: azurescending
Last seen: 10 years, 4 months
Re: using spawn to.... well, spawn [Re: JssMthrFcknChrst]
    #1254779 - 01/28/03 09:03 AM (15 years, 3 months ago)

I never go past 3 generations - as stamets suggests in TMC.

However, I sometimes take my agar isolations a bit further.


--------------------
Raadt

-- The information I provide is only information from readings, growing of gourmet mushrooms, and second hand stories--


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Amazon Shop: Agar, Paul Stamets

Mushrooms, Mycology and Psychedelics >> Mushroom Cultivation

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