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badman


Registered: 06/14/06
Posts: 4,039
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'Decent' sclerotia yeilds
#12544013 - 05/11/10 04:24 PM (14 years, 8 months ago) |
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What is a decent yeild off a sclerotia producing species?
I know yield questions piss a lot of people off but in this instance I think it is a valid question as the whole process is in vitro and much more controlled.
I want to know your yields using pure cultures only, WRT a particular species. Hopefully from this I can gauge how good my cultures are and basically to tell me if a better culture can be obtained. Ideally the more numbers yon give the better.
For example, my P. mexicana isolate yields 150 g off 216 g (800 ml) of dry RGS in 12 week. This is but what bout Alt#7 and P. atlantis?
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whyblameus
on a mission



Registered: 06/02/09
Posts: 11,440
Loc: Ca,Ga,Id wanna trade LOL
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Re: 'Decent' sclerotia yeilds [Re: badman]
#12544105 - 05/11/10 04:39 PM (14 years, 8 months ago) |
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i harvested 52.2 grams from a quart 3/4 full of wbs at about 5 weeks.it was a iso of atl#7.
i have another quart im gonna harvest in a little over a week making it around 8 weeks ill post it here if this is still going.
-------------------- if you dont got no one to hate on feel free to hate on me!
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badman


Registered: 06/14/06
Posts: 4,039
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Re: 'Decent' sclerotia yeilds [Re: whyblameus]
#12544324 - 05/11/10 05:11 PM (14 years, 8 months ago) |
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Im guessing thats about 350 g of dry WBS but still is that good for an Alt#7 isolate? I dunno and that is the question. The more yields the merrier.
Are you still testing various isolates?
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whyblameus
on a mission



Registered: 06/02/09
Posts: 11,440
Loc: Ca,Ga,Id wanna trade LOL
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Re: 'Decent' sclerotia yeilds [Re: badman]
#12579333 - 05/17/10 02:17 PM (14 years, 8 months ago) |
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Quote:
badman said: Im guessing thats about 350 g of dry WBS but still is that good for an Alt#7 isolate? I dunno and that is the question. The more yields the merrier.
Are you still testing various isolates?
i dont know if it was a good yield since it was my first jar i had done.
i only had that one iso and im not working on various isos.
i just harvested another jar of the atl iso at around 7.5 weeks and got 49 grams fresh.
less then the jar that i harvested at 5 weeks
and i thought stones were 1/3 moisture. i dried them and it was less then half the weight.(about 18 grams)
-------------------- if you dont got no one to hate on feel free to hate on me!
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jingus

Registered: 05/04/09
Posts: 1,669
Loc: Oregon, U.S.
Last seen: 11 years, 11 months
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Re: 'Decent' sclerotia yeilds [Re: whyblameus]
#12579394 - 05/17/10 02:26 PM (14 years, 8 months ago) |
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yeah please keep updating this post. i know i will in a month or two
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badman


Registered: 06/14/06
Posts: 4,039
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Re: 'Decent' sclerotia yeilds [Re: jingus]
#12580878 - 05/17/10 06:14 PM (14 years, 8 months ago) |
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I thought the were 1/3 biomass that makes sense with my mex a, there abouts.
that 49 g off the same amount of wbs?
Need more peoples input, there has to be more people with alt7 isolates, you know what i mean.
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whyblameus
on a mission



Registered: 06/02/09
Posts: 11,440
Loc: Ca,Ga,Id wanna trade LOL
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Re: 'Decent' sclerotia yeilds [Re: badman]
#12580934 - 05/17/10 06:21 PM (14 years, 8 months ago) |
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Quote:
I thought the were 1/3 biomass that makes sense with my mex a, there abouts.
your to damn smart.when i talk to you i feel dumb i dont know what biomass is would that mean 1/3 moisture or 1/3 mush matter?
Quote:
that 49 g off the same amount of wbs?
pretty much i just fill the jars with grain by eye.both were quart jars.
Quote:
Need more peoples input, there has to be more people with alt7 isolates, you know what i mean.
yea i know im not the only one
-------------------- if you dont got no one to hate on feel free to hate on me!
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stonesun
Sclerotia Aficionado




Registered: 05/22/09
Posts: 5,444
Loc: 64º08'00"N 21º56'00"W
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Re: 'Decent' sclerotia yeilds [Re: whyblameus]
#12581143 - 05/17/10 06:55 PM (14 years, 8 months ago) |
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I have to pull up my records, will report back later.
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badman


Registered: 06/14/06
Posts: 4,039
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Re: 'Decent' sclerotia yeilds [Re: whyblameus]
#12581177 - 05/17/10 07:01 PM (14 years, 8 months ago) |
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Im not that smart, yet. Mush matter man
I might even have a really good alt7 iso due to some massive naming errors in Holland which is good and bad. Good cos someones done the leg work and bad cos they sold it as P. atlantis.
Quote:
stonesun said: I have to pull up my records, will report back later.
Yes mate!
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Shaggy Shaman
Divine Conduit



Registered: 11/01/09
Posts: 348
Loc: United States
Last seen: 2 years, 6 hours
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Re: 'Decent' sclerotia yeilds [Re: badman]
#12581549 - 05/17/10 08:08 PM (14 years, 8 months ago) |
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I just got 115.2 grams wet from a single Mex-A quart jar, filled 3/4 full with WBS... but it wasn't an ISO. Still, it was good enough I sent a sample to a buddy for him to play with and maybe get a good ISO.
-------------------- "Disbelief in magic can force a poor soul into believing in government and business."
— Tom Robbins
“The true value of a human being can be found in the degree to which he has attained liberation from the self"
-- Albert Einstein
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badman


Registered: 06/14/06
Posts: 4,039
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That is a good yield, you know i will allow MS cos it still will give me an idea of how a culture performs.
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Doc_T
Random Dude




Registered: 03/06/09
Posts: 42,395
Loc: Colorado
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Re: 'Decent' sclerotia yeilds [Re: badman]
#12581979 - 05/17/10 09:19 PM (14 years, 8 months ago) |
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I just use multispore ATL. Yields on grain are ok, better spawned.
-------------------- You make it all possible. Doesn't it feel good?
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linkin17222
Stranger


Registered: 12/23/09
Posts: 266
Last seen: 5 years, 10 months
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Re: 'Decent' sclerotia yeilds [Re: Doc_T]
#12583106 - 05/18/10 12:34 AM (14 years, 8 months ago) |
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Can you spawn sclerotia in coir?
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Shaggy Shaman
Divine Conduit



Registered: 11/01/09
Posts: 348
Loc: United States
Last seen: 2 years, 6 hours
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Re: 'Decent' sclerotia yeilds [Re: linkin17222]
#12584219 - 05/18/10 08:05 AM (14 years, 7 months ago) |
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Quote:
linkin17222 said: Can you spawn sclerotia in coir?
yeah, you can get the stones from the jar and then spawn the grain to coir and in a couple months get more stones or even fruit it. I'm on a small hiatus right now though, trying to break the fungus gnat cycle
-------------------- "Disbelief in magic can force a poor soul into believing in government and business."
— Tom Robbins
“The true value of a human being can be found in the degree to which he has attained liberation from the self"
-- Albert Einstein
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Shaggy Shaman
Divine Conduit



Registered: 11/01/09
Posts: 348
Loc: United States
Last seen: 2 years, 6 hours
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Re: 'Decent' sclerotia yeilds [Re: badman]
#12584234 - 05/18/10 08:08 AM (14 years, 7 months ago) |
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Quote:
badman said: That is a good yield, you know i will allow MS cos it still will give me an idea of how a culture performs.
I was thinking that as well, but wasn't sure what you were meaning by "pure" cultures.
-------------------- "Disbelief in magic can force a poor soul into believing in government and business."
— Tom Robbins
“The true value of a human being can be found in the degree to which he has attained liberation from the self"
-- Albert Einstein
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badman


Registered: 06/14/06
Posts: 4,039
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Re: 'Decent' sclerotia yeilds [Re: Doc_T]
#12584534 - 05/18/10 09:21 AM (14 years, 7 months ago) |
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Quote:
Doc_T said: I just use multispore ATL. Yields on grain are ok, better spawned.
Why only MS doc? Id rather keep the whole process in vitro for 4 odd months, the quality of stone, taste and texture seems better. Theres no chance of fuckery in vitro, i have an MS alt7 jar but form the surface stoning yields look lame ill spawn that in a few months.
ive just cased ~150 g of mex a stones, from a jar dated november 13th, for prints but im not feeling confident, so minor.
Pure cultures, monocultures, isolates all inter changeable terms
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