Home | Community | Message Board


This site includes paid links. Please support our sponsors.


Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Shop: Myyco.com Isolated Cubensis Liquid Culture For Sale   Left Coast Kratom Buy Kratom Extract   Original Sensible Seeds High THC Strains   North Spore Injection Grain Bag   PhytoExtractum Buy Bali Kratom Powder   Mushroom-Hut Mono Tub Substrate

Jump to first unread post Pages: 1
Some of these posts are very old and might contain outdated information. You may wish to search for newer posts instead.
Offlinedirtlambguy
Stranger
Male
Registered: 07/23/09
Posts: 222
Loc: NC, USA
Last seen: 12 years, 7 months
Another Strain Isolation Question
    #12540414 - 05/10/10 10:15 PM (13 years, 10 months ago)

And,

I know its possible to start a strain isolation project via spore print, mushroom tissue, or colonized grain.  I was planning on starting my project with mushroom tissue, but I have a spore print and a jar of colonized grain.  Is it best to start a project with a cloned mushroom, or does it matter? 

-Nig


--------------------
Dirtlamb makes you smart!

Extras: Filter Print Post Top
InvisibleDoc_T
Random Dude
Male User Gallery


Folding@home Statistics
Registered: 03/06/09
Posts: 42,395
Loc: Colorado Flag
Re: Another Strain Isolation Question [Re: dirtlambguy]
    #12540426 - 05/10/10 10:16 PM (13 years, 10 months ago)

'best' to start from spores, but any of those will work well.


--------------------
You make it all possible. Doesn't it feel good?

Extras: Filter Print Post Top
InvisibleJitsu
JKD Love
Male User Gallery


Registered: 11/12/08
Posts: 1,073
Re: Another Strain Isolation Question [Re: Doc_T]
    #12540484 - 05/10/10 10:25 PM (13 years, 10 months ago)

With spores, you will have more options to work with and you will obtain true isolates. The drawback is that you will be spending more time obtaining single sector isolates from spores as opposed to clones. I really enjoy agar work though so I tend to just use spores.


--------------------
Do not deny the classical approach, simply as a reaction, or you will have created another pattern and trapped yourself there.

How I get my Pinsets
The Capabilities Of A Shotgun FC

Extras: Filter Print Post Top
Offlinenugjug
Wanderer

Registered: 11/07/05
Posts: 703
Last seen: 6 years, 1 month
Re: Another Strain Isolation Question [Re: dirtlambguy]
    #12540490 - 05/10/10 10:26 PM (13 years, 10 months ago)

Cloning from a fruit body is easier and faster because you know it will be a fruiting strain.  That being said Doc is right about spores being best as you have more genetic variation to pick from.  However picking is the problem.  Once you isolate several strains you have to fruit them individually to test for potency, growth rates, size of flushes, etc.

Edit: Jitsu beat me to it.

Edited by nugjug (05/10/10 10:27 PM)

Extras: Filter Print Post Top
InvisibleSeagoat
~
 User Gallery

Registered: 04/07/10
Posts: 306
Re: Another Strain Isolation Question [Re: nugjug]
    #12540569 - 05/10/10 10:40 PM (13 years, 10 months ago)

I remember reading something about: If a sector has rizomorphic growth then it has the proper genetics to fruit. Is this correct? If so then you would know if your sector is capable of fruiting, because you wouldn't isolate a sector that isn't rizomorphic anyways.

Extras: Filter Print Post Top
OfflineGiggle_Grower
A lil less noob each day
Male User Gallery


Registered: 04/07/10
Posts: 1,598
Loc: Shroomery
Last seen: 1 year, 7 months
Re: Another Strain Isolation Question [Re: Seagoat]
    #12540585 - 05/10/10 10:43 PM (13 years, 10 months ago)

i dont think all rhizo can fruit, but its pretty rare to find a non fruiting 'strain'. just my .02


--------------------
I'm  always interested in trades.
The Awesome Purple Mystics
Noobs! The best tool here is up top to the right. Don't  forget about it. Just type your question in!:cool:
RR is my favorite source of knowledge. Check out his videos!

If I forgot to leave you a rating, please remind me.

Extras: Filter Print Post Top
InvisibleJitsu
JKD Love
Male User Gallery


Registered: 11/12/08
Posts: 1,073
Re: Another Strain Isolation Question [Re: Giggle_Grower]
    #12540633 - 05/10/10 10:51 PM (13 years, 10 months ago)

Rhizomorph is said to be the most prolific pinning type of mycelium for cubensis, but some of my best pinning strains (isolates) are tomentose appearing in growth. I've isolated many strains over the last couple years and have yet to see one of them not fruit.

Pretty sure it just comes down to compatible hyphae, rather than the growth pattern.(as far as fruiting strains go)


--------------------
Do not deny the classical approach, simply as a reaction, or you will have created another pattern and trapped yourself there.

How I get my Pinsets
The Capabilities Of A Shotgun FC

Edited by Jitsu (05/10/10 10:57 PM)

Extras: Filter Print Post Top
InvisibleSeagoat
~
 User Gallery

Registered: 04/07/10
Posts: 306
Re: Another Strain Isolation Question [Re: Jitsu]
    #12540687 - 05/10/10 11:03 PM (13 years, 10 months ago)

Quote:

Jitsu said:
Rhizomorph is said to be the most prolific pinning type of mycelium for cubensis, but some of my best pinning strains (isolates) are tomentose appearing in growth. I've isolated many strains over the last couple years and have yet to see one of them not fruit.

Pretty sure it just comes down to compatible hyphae, rather than the growth pattern.(as far as fruiting strains go)




Cool, thanks for sharing. I'm about to get my hands dirty with some agar isolations.

Extras: Filter Print Post Top
Jump to top Pages: 1

Shop: Myyco.com Isolated Cubensis Liquid Culture For Sale   Left Coast Kratom Buy Kratom Extract   Original Sensible Seeds High THC Strains   North Spore Injection Grain Bag   PhytoExtractum Buy Bali Kratom Powder   Mushroom-Hut Mono Tub Substrate


Similar ThreadsPosterViewsRepliesLast post
* strain isolation effect on yield Dorin 2,450 6 11/16/01 04:17 PM
by Olgualion
* Strain isolation, and agar cultures amanmusk 2,316 2 03/25/02 10:38 PM
by HisStudent
* Strain/Rice question Romulusguy 553 2 05/21/02 02:42 AM
by angryshroom
* Strain - High Question Pulseczar 2,880 15 02/11/02 06:27 PM
by queerbandit
* Strain Comparison **UPDATE** The_Mad_Hatter 2,180 12 08/20/02 11:23 PM
by The_Mad_Hatter
* Strain purity an issue? Zebedee 769 2 03/01/02 04:31 PM
by Azure
* Todays stupid question. Magash 1,702 11 08/01/02 06:23 PM
by Magash
* Yest another harvesting question. mk31762 1,578 5 11/28/01 08:02 PM
by DaBeerLover

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: Shroomism, george castanza, RogerRabbit, veggie, mushboy, fahtster, LogicaL Chaos, 13shrooms, Stipe-n Cap, Pastywhyte, bodhisatta, Tormato, Land Trout, A.k.a
821 topic views. 22 members, 142 guests and 58 web crawlers are browsing this forum.
[ Show Images Only | Sort by Score | Print Topic ]
Search this thread:

Copyright 1997-2024 Mind Media. Some rights reserved.

Generated in 0.029 seconds spending 0.009 seconds on 14 queries.