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Genome Evolution | First, a Bang Then, a Shuffle
    #1252727 - 01/27/03 10:29 AM (14 years, 30 days ago)

For the Science Lover in you! :smile:

Picture an imperfect hall of mirrors, with gene sequences reflecting wildly: That's the human genome. The duplications that riddle the genome range greatly in size, clustered in some areas yet absent in others, residing in gene jungles as well as within vast expanses of seemingly genetic gibberish. And in their organization lie clues to genome origins. "We've known for some time that duplications are the primary force for genes and genomes to evolve over time," says Evan Eichler, director of the bioinformatics core facility at the Center for Computational Genomics, Case Western Reserve University, Cleveland.

For three decades, based largely on extrapolations from known gene families in humans, researchers have hypothesized two complete genome doublings--technically, polyploidization--modified by gene loss, chromosome rearrangements, and additional limited duplications. But that view is changing as more complete evidence from genomics reveals a larger role for recent small-scale changes, superimposed on a probable earlier single doubling. Ken Wolfe, a professor of genetics at the University of Dublin, calls the new view of human genome evolution "the big bang" followed by "the slow shuffle."

It's a controversial area.

"There has been a lot of debate about whether there were two complete polyploid events at the base of the vertebrate lineages. The main problem is that vertebrate genomes are so scrambled after 500 million years, that it is very difficult to find the signature of such an event," explains Michael Lynch, a professor of biology at Indiana University, Bloomington, With accumulating sequence data from gene families, a picture is emerging of a lone, complete one-time doubling at the dawn of vertebrate life, followed by a continual and ongoing turnover of about 5-10% of the genome that began in earnest an estimated 30-50 million years ago. Short DNA sequences reinvent themselves, duplicating and sometimes diverging in function and dispersing among the chromosomes, so that the genome is a dynamic, ever-changing entity.

Duplication in the human genome is more extensive than it is in other primates, says Eichler. About 5% of the human genome consists of copies longer than 1,000 bases. Some doublings are vast. Half of chromosome 20 recurs, rearranged, on chromosome 18. A large block of chromosome 2's short arm appears again as nearly three-quarters of chromosome 14, and a section of its long arm is also on chromosome 12. The gene-packed yet diminutive chromosome 22 sports eight huge duplications. "Ten percent of the chromosome is duplicated, and more than 90% of that is the same extremely large duplication. You don't have to be a statistician to realize that the distribution of duplications is highly nonrandom," says Eichler.

The idea that duplications provide a mechanism for evolution is hardly new. Geneticists have long regarded a gene copy as an opportunity to try out a new function while the original sequence carries on. More often, though, the gene twin mutates into a nonfunctional pseudogene or is lost, unconstrained by natural selection because the old function persists. Or, a gene pair might diverge so that they split a function.

Some duplications cause disease. A type of Charcot-Marie-Tooth disease, for example, arises from a duplication of 1.5 million bases in a gene on chromosome 17. The disorder causes numb hands and feet.


A duplication's size and location may hold clues to its origin. A single repeated gene is often the result of a tandem duplication, which arises when chromosomes misalign during meiosis, and crossing over distributes two copies of the gene (instead of one) onto one chromosome. This is how the globin gene clusters evolved, for example. "Tandem duplicates are tandemly arranged, and there may be a cluster of related genes located contiguously on the chromosome, with a variable number of copies of different genes," says John Postlethwait, professor of biology in the Institute of Neuroscience at the University of Oregon, who works on the zebrafish genome.

In contrast to a tandem duplication, a copy of a gene may appear on a different chromosome when messenger RNA is reverse-transcribed into DNA that inserts at a new genomic address. This is the case for two genes on human chromosome 12, called PMCHL1 and PMCHL2, that were copied from a gene on chromosome 5 that encodes a neuropeptide precursor. Absence of introns in the chromosome 12 copies belies the reverse transcription, which removes them.1 (Tandem duplicates retain introns.)

The hallmarks of polyploidy are clear too: Most or all of the sequences of genes on one chromosome appears on another. "You can often still see the signature of a polyploidization event by comparing the genes on the two duplicated chromosomes," Postlethwait says.

Muddying the waters are the segmental duplications, which may include tandem duplications, yet also resemble polyploidy. "Instead of a single gene doubling to make two adjacent copies as in a tandem duplication, in a segmental duplication, you could have tens or hundreds of genes duplicating either tandemly, or going elsewhere on the same chromosome, or elsewhere on a different chromosome. If the two segments were on different chromosomes, it would look like polyploidization for this segment," says Postlethwait. Compounding the challenge of interpreting such genomic fossils is that genetic material, by definition, changes. "As time passes, the situation decays. Tandem duplicates may become separated by inversions, transpositions, or translocations, making them either distant on the same chromosome or on different chromosomes," he adds.


Many vertebrate genomes appear to be degenerate tetraploids, survivors of a quadrupling--a double doubling from haploid to diploid to tetraploid--that left behind scattered clues in the form of genes present in four copies. This phenomenon is called the one-to-four rule. Wolfe compares the scenario to having four decks of cards, throwing them up in the air, discarding some, selecting 20, and then trying to deduce what you started with. Without quadruples in the sample, it is difficult to infer the multideck origin. So it is for genes and genomes.

"How can you tell whether large duplications built up, or polyploidy broke down? People are saying that they can identify blocks of matching DNA that are evidence for past polyploidization, which have been broken up and overlain by later duplications. But at what point do blocks just become simple duplications?" asks Susan Hoffman, associate professor of zoology at Miami University, Oxford, Ohio.

The idea that the human genome has weathered two rounds of polyploidy, called the 2R hypothesis, is attributed to Susumu Ohno, a professor emeritus of biology at City of Hope Medical Center in Duarte, Calif.2 The first whole genome doubling is postulated to have occurred just after the vertebrates diverged from their immediate ancestors, such as the lancelet (Amphioxus). A second full doubling possibly just preceded the divergence of amphibians, reptiles, birds, and mammals from the bony fishes.

Evidence for the 2R hypothesis comes from several sources. First, polyploidy happens. The genome of flowering plants doubled twice, an estimated 180 and 112 million years ago, and rice did it again 45 million years ago.3 "Plants have lots of large blocks of chromosomal duplications, and the piecemeal ones originated at the same time," indicating polyploidization, says Lynch. The yeast Saccharomyces cerevisiae is also a degenerate tetraploid, today bearing the remnants of a double sweeping duplication.4

Polyploidy is rarer in animals, which must sort out unmatched sex chromosomes, than in plants, which reproduce asexually as well as sexually. "But polyploidization is maintained over evolutionary time in vertebrates quite readily, although rarely. Recent examples, from the last 50 million years ago or so, include salmonids, goldfish, Xenopus [frogs], and a South American mouse," says Postlethwait. On a chromosomal level, polyploidy may disrupt chromosome compatibility, but on a gene level, it is an efficient way to make copies. "Polyploidy solves the dosage problem. Every gene is duplicated at the same time, so if the genes need to be in the right stoichiometric relationship to interact, they are. With segmental duplications, gene dosages might not be in the same balance. This might be a penalty and one reason why segmental genes don't survive as long as polyploidy," Lynch says.

Traditional chromosome staining also suggests a double doubling in the human genome's past, because eight chromosome pairs have near-doppleg?ngers, in size and band pattern.5 A flurry of papers in the late 1990s found another source of quadrupling: Gene counts for the human, then thought to be about 70,000, were approximately four times those predicted for the fly, worm, and sea squirt. The human gene count has since been considerably downsized.

Finally, many gene families occur in what Jurg Spring, a professor at the University of Basel's Institute of Zoology in Switzerland, dubs "tetrapacks."6 The HOX genes, for example, occupy one chromosome in Drosophila melanogaster but are dispersed onto four chromosomes in vertebrate genomes.7 Tetrapacks are found on every human chromosome, and include zinc-finger genes, aldolase genes, and the major histocompatibility complex genes.

"In the 1990s, the four HOX clusters formulated the modern version of the 2R model, that two rounds of genome duplication occurred, after Amphioxus and before bony fishes," explains Xun Gu, an associate professor of zoology and genetics at Iowa State University in Ames. "Unfortunately, because of the rapid evolution of chromosomes as well as gene losses, other gene families generated in genome projects did not always support the classic 2R model. So in the later 1990s, some researchers became skeptical of the model and argued the possibility of no genome duplication at all."


Human genome sequence information has enabled Gu and others to test the 2R hypothesis more globally, reinstating one R. His group used molecular-clock analyses to date the origins of 1,739 duplications from 749 gene families.8 If these duplications sprang from two rounds of polyploidization, the dates should fall into two clusters. This isn't exactly what happened. Instead, the dates point to a whole genome doubling about 550 million years ago and a more recent round of tandem and segmental duplications since 80 million years ago, when mammals radiated.

Ironically, sequencing of the human genome may have underestimated the number of duplications. The genome sequencing required that several copies be cut, the fragments overlapped, and the order of bases derived. The algorithm could not distinguish whether a particular sequence counted twice was a real duplication, present at two sites in the genome, or independent single genes obtained from two of the cut genomes.

Eichler and his group developed a way around this methodological limitation. They compare sequences at least 15,000 bases long against a random sample of shotgunned whole genome pieces. Those fragments that are overrepresented are inferred to be duplicated.8 The technique identified 169 regions flanked by large duplications in the human genome.

Although parts of the human genome retain a legacy of a long-ago total doubling, the more recent, smaller duplications provide a continual source of raw material for evolution. "My view is that both happen. A genome can undergo polyploidy, duplicating all genes at once, but the rate of segmental duplications turns out to be so high that every gene will have had the opportunity to duplicate" by this method also, concludes Lynch. It will be interesting to see how the ongoing analyses of the human and other genome sequences further illuminate the origins and roles of duplications.

1. A. Courseaux, J.-L. Nahon, "Birth of 2 chimeric genes in the Hominidae lineage," Science, 291:1293-7, 2001.

2. S. Ohno, Evolution by Gene Duplication, Heidelberg, Germany: Springer-Verlag, 1970.

3. J. Bennetzen, "Opening the door to comparative plant biology," Science, 296:60-3, 2002.

4. A. Wagner, "Asymmetric functional divergence of duplicated genes in yeast," Molec Biol Evol, 19:1760-8, October 2002.

5. D.E. Comings, "Evidence for ancient tetraploidy and conservation of linkage groups in mammalian chromosomes," Nature, 238:455-7, 1972.

6. J. Spring, "Genome duplication strikes back," Nat Genet, 31:128-9, 2002.

7. D. Larhammar et al., "The human hox-bearing chromosome regions did arise by block or chromosome (or even genome) duplications," Genome Res, 12:1910-20, December 2002.

8. X. Gu et al., "Age distribution of human gene families shows significant roles of both large-and small-scale duplications in vertebrate evolution," Nat Genet, 31:205-9, 2002.

9. J.A. Bailey et al., "Recent segmental duplications in the human genome," Science, 297:1003-7, Aug. 9, 2002.


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Re: Genome Evolution | First, a Bang Then, a Shuffle [Re: ]
    #1253154 - 01/27/03 12:31 PM (14 years, 30 days ago)

Excellent post.

It'll be interesting to see where they are in 5 or 10 years with this same topic.

Happy mushrooming!

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Eggshell Walker

Registered: 01/19/00
Posts: 15,413
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Re: Genome Evolution | First, a Bang Then, a Shuffle [Re: ]
    #1253184 - 01/27/03 12:36 PM (14 years, 30 days ago)

Picture an imperfect hall of mirrors, with gene sequences reflecting wildly: That's the human genome.

Thought that was Bruce Lee in "Enter the Dragon"...


The proof is in the pudding.

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Re: Genome Evolution | First, a Bang Then, a Shuffle [Re: ToxicMan]
    #1253238 - 01/27/03 12:47 PM (14 years, 30 days ago)

Thanks TM, I thought of you when I created it. :smile:

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Reality Hacker

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Re: Genome Evolution | First, a Bang Then, a Shuffle [Re: ]
    #1254085 - 01/27/03 05:54 PM (14 years, 30 days ago)

...when I created it.

Copy and Paste, or did you write this essay?

What if everything around you
Isn't quite as it seems?
What if all the world you think you know,
Is an elaborate dream?
And if you look at your reflection,
Is it all you want it to be?

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Registered: 02/21/01
Posts: 36,253
Loc: Lost in the Patterns
Re: Genome Evolution | First, a Bang Then, a Shuffle [Re: ]
    #1265454 - 01/31/03 12:04 PM (14 years, 26 days ago)

Mr. Mushrooms-
                  That was a wonderful post. Gets my mind thinking and polyploidy(along with allopolyploids: two species combining with different number of chromosomes to form a new organism...like two different strains of wheat in Kansas one with 8 chromosomes and the other with 10...they found a huge patch with 18 chromosomes...crazy and the hybrid is fertile too) are very interesting subjects. Your post had many good points and there are some things I'd like to mention that I thought would interest you.

To start off, the presence of introns and exons in our DNA makes everything ten million times harder to grasp and learn. It is going to complicate our understanding of DNA very much, at least in my opinion. Alternative RNA Splicing can yield various RNA transcripts from the same gene. Depending on which sections are treated as exons and which ones are treated as introns (through regulatory proteins), a gene can basically function as more than one gene...kinda throws the old one gene-one polypeptide theory out the window. Drosophila is an excellent example of this, where the sex differences of it are largely determined by how the RNA from certain genes is spliced (what serves as exons and introns).

Also, different exons of a gene tend to code for a certain domain of the corresponding protein. Domains are parts of the protein, sometimes an independent polypeptide, which each have a different function (like the binding domain which binds to certain substrates, the active domain which modifies certain substrates, etc.). If these exons were to being duplicated in various random parts throughout the genome, new useful proteins could form as a result. This is made possible by interspersed repetitive DNA as opposed to tandemly repetitive DNA. An example of interspersed repetitive DNA would be the Alu Elements, which appear many times throughout a cell's genome and their function is unknown. These "jumping" genes, as they were originally called, or  transposons  are the key behind those duplications and why there are so many random duplications throughout the human genome.

Tandem duplicates may become separated by inversions, transpositions, or translocations, making them either distant on the same chromosome or on different chromosomes," he adds. 

The most simple transposons are nothing but the sequence for the enzyme, transposase, which catalyzes the transposons movement, and an inverted repeat on both sides of the gene. Composite transposons carry genes inbetween two transposase sequences. Transposons make up about 10% of the human genome! They also make up 50% of the corn (maize) genome which is where they were first discovered by Barbara McClintock in the 1950's.


This is how the globin gene clusters evolved, for example.

Actually, Campbell says that they globin gene clusters were a result of transposition because they reside on two different chromosomes...they each underwent a few mutations after their duplication.

Retrotransposons are like transposons except that the gene is transcribed and travels via an RNA intermediate. It is then reverse transcribed back into the genome elsewhere, by the use of reverse transcriptase which the retrotransposon codes for itself. Retrotransposons are thought to be the origination of retroviruses and they very much explain the quote of yours:

a copy of a gene may appear on a different chromosome when messenger RNA is reverse-transcribed into DNA that inserts at a new genomic address. This is the case for two genes on human chromosome 12, called PMCHL1 and PMCHL2, that were copied from a gene on chromosome 5 that encodes a neuropeptide precursor. Absence of introns in the chromosome 12 copies belies the reverse transcription, which removes them.1 (Tandem duplicates retain introns.) 

That information about gene quadrupling among animals is very cool, and in my bio book they show a picture of a red viscacha rat from Argentina that is a tetraploid. Fittingly, its sperm's head is quite large to encapsulate all those chromosomes.

I really enjoyed your article and just wanted to show you how much of the shuffling part of the theory was result of transposons. Also wanted to thank you for writing a such an interesting article. I enjoy genetics very much and may plan on going into it.  :grin:

need that cash to feed them jones.

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Re: Genome Evolution | First, a Bang Then, a Shuffle [Re: Teragon]
    #1265822 - 01/31/03 02:16 PM (14 years, 26 days ago)

Thank you Teragon! :smile:

Yes, the gene-one polypeptide theory is phasing out thanks to the fruitfly work and others being done.  Do you have a source that I can read about Campbell's work in reference to the transposition causing the globin gene clusters? 

Retrotransposons could very well be the origination of retroviruses and it dovetails nicely into the fact that PMCHL1 and PMCHL2 were copied fron gene 5 as they were.

Welcome to Spirituality, Philosophy, and Science forum! :smile:

At some point we are thinking of redesigning the link stickies and combining them.  Before that happens I think we need a Science Link thread.  I hope you would consider contributing to it.

Cheers :smile:   

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Folding@home Statistics
Registered: 07/13/99
Posts: 4,805
Loc: On the Brink of Madness
Re: Genome Evolution | First, a Bang Then, a Shuffle [Re: ]
    #1266976 - 01/31/03 10:48 PM (14 years, 26 days ago)

I'm just wondering... have they been able to do a DNA analysis of the recently discovered Neandertal man that was found frozen up in the mountains of Europe (the Alps? I can't remember right now.)?

I think that would help shed some light on the "nonrandomness" of the human genome. (BTW, human DNA is something like 5% different [EDIT: thanks Joshua] from chimpanzee DNA... I guess Eichler ignores that, as well as mitochondrial DNA similarities)

Note: In desperate need of a cure...

Edited by Sclorch (02/01/03 08:39 AM)

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Registered: 10/27/98
Posts: 5,389
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Re: Genome Evolution | First, a Bang Then, a Shuffle [Re: Sclorch]
    #1267004 - 01/31/03 11:29 PM (14 years, 26 days ago)

"BTW, human DNA is something like 95% different from chimpanzee DNA"

I think it is more like 95% similar.


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