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Invisibleresonance77
Stranger


Registered: 03/01/10
Posts: 103
tubs colonizing slowly , might be ready, new pics 5/13
    #12492897 - 05/02/10 10:14 AM (14 years, 8 months ago)

I have 3 18 gallon rubbermaid roughnecks set up mono style. I spawned 7 quarts of fully colonized wbs to the first one on 4/18. The other two were spawned with 7 quarts each of fully colonized wbs on the 24th. I followed Damion's elementary coir Tek exactly, except I used micropore tape on the top two holes due to my lids being tight. I switched to duct tape 4 days ago, as suggested by Damion in his Tek thread. Since then, everything just about stalled right out, or is at least creeping along very slowly.

The first tub is on day 14 and is at about 60%-70%. The other two tubs are on day 8 and I just checked them today. They only have a few small spots of white poking up. I would guess around 5% of the surface.

There isn't any signs of contams at all. It doesn't look dried out at all. It wasn't too wet to begin with. The spawn jars looked killer, thick white 100%. I spawned to clean tubs in a still air environment. Oust bombed the room 20 minutes prior to spawning. Wore clean gloves. Took a shower and put on clean clothes right before. Used alcohol on everything prior to working (gloves, tub, outside of the jars, etc.) I'm sure shit was clean.

If it matters, the first 7 jars were Cambodian and colonized the wbs in 18 days. The other two batches of jars were Penis Envy colonized in 21, and La Premavera colonized in 19 days. The tubs are in trash bags at room temp (fluctuates from 65 to 75 degrees depending on the day.)

This isn't a patience issue. I can wait forever. I'm just worried because it seems like from everything I've read here, they should be further along by now. Is there anything I'm overlooking and when should I start worrying?

Edited by resonance77 (05/13/10 11:12 AM)

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Invisibledancefloordale
Research Assistant


Registered: 02/13/09
Posts: 2,522
Re: tub colonizing slowly [Re: resonance77]
    #12492932 - 05/02/10 10:24 AM (14 years, 8 months ago)

Give it some more time, those numbers aren't that out of the ordinary. Is there any smell emitting from the tubs at all, like sour odor?

could be a moisture content issue, which can really effect colonization time.


--------------------
Everything posted by the user dancefloordale, aside from what is written in this here clause is completely and utterly fictitious, despite any information read (or seen), above (or below) that might lead you to believe otherwise.

Hydra Tek - A detailed guide, for newest to the most skilled cultivators.

HCA

Bulk growing made easy-discussion
Bulk Growing Made Easy

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Invisibleresonance77
Stranger


Registered: 03/01/10
Posts: 103
Re: tub colonizing slowly [Re: dancefloordale]
    #12492983 - 05/02/10 10:35 AM (14 years, 8 months ago)

Quote:

dancefloordale said:
Give it some more time, those numbers aren't that out of the ordinary. Is there any smell emitting from the tubs at all, like sour odor?

could be a moisture content issue, which can really effect colonization time.




They smell fine. Nothing out of the ordinary.

The moisture content seemed right on to start with. I'm not going to open the tubs to check it now, but from what I can see through the Plexiglas window on the lid, it doesn't look dry at all. There are tiny beads of condensation on the myc that has popped up and on the sides and lid of the tub as well. The rest of the sub still looks dark, not dry.

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Invisiblebotanisthype
P$ylocibe$ounDW@vE!
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Registered: 07/31/03
Posts: 839
Loc: DECEPTICON MANOR
Re: tub colonizing slowly [Re: resonance77]
    #12492990 - 05/02/10 10:37 AM (14 years, 8 months ago)

High co2 helps incubate well before fruiting.....after mixing spawn/sub in tubs i just duct tape all the holes w/gorilla tap and leave them at 80 degress . in 5-6 days for 71 quart tub to fully colonize ...

your using cambos?! they grow fast well too, bu it seems your abit behind , wha size jars are you using for it take that long, are you using Ms or Lc?!

your tubs could be susceptable to contamination due to being in there for too long,
one time i had a tub coonizing for almost weeks it took for ever and when i fruited it i saw trich coming from the bottom ..bleh..

did u pasturize your coir well?!


--------------------
Please +REP if i've been of any help... thanks!!!

Dinosaur of the Funny Family, though I have short arms, they fit in my GB... lol

Edited by botanisthype (05/02/10 10:41 AM)

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Invisibledancefloordale
Research Assistant


Registered: 02/13/09
Posts: 2,522
Re: tub colonizing slowly [Re: resonance77]
    #12493009 - 05/02/10 10:40 AM (14 years, 8 months ago)

Quote:

resonance77 said:
Quote:

dancefloordale said:
Give it some more time, those numbers aren't that out of the ordinary. Is there any smell emitting from the tubs at all, like sour odor?

could be a moisture content issue, which can really effect colonization time.




They smell fine. Nothing out of the ordinary.

The moisture content seemed right on to start with. I'm not going to open the tubs to check it now, but from what I can see through the Plexiglas window on the lid, it doesn't look dry at all. There are tiny beads of condensation on the myc that has popped up and on the sides and lid of the tub as well. The rest of the sub still looks dark, not dry.




Cool, just wait it out and see what happens. Agreed, the last thing you want to do is open the tubs.


--------------------
Everything posted by the user dancefloordale, aside from what is written in this here clause is completely and utterly fictitious, despite any information read (or seen), above (or below) that might lead you to believe otherwise.

Hydra Tek - A detailed guide, for newest to the most skilled cultivators.

HCA

Bulk growing made easy-discussion
Bulk Growing Made Easy

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Invisibleresonance77
Stranger


Registered: 03/01/10
Posts: 103
Re: tub colonizing slowly [Re: botanisthype]
    #12493051 - 05/02/10 10:49 AM (14 years, 8 months ago)

Quote:

botanisthype said:
High co2 helps incubate well before fruiting.....after mixing spawn/sub in tubs i just duct tape all the holes w/gorilla tap and leave them at 80 degress . in 5-6 days for 71 quart tub to fully colonize ...

your using cambos?! they grow fast well too, bu it seems your abit behind , wha size jars are you using for it take that long, are you using Ms or Lc?!

your tubs could be susceptable to contamination due to being in there for too long,
one time i had a tub coonizing for almost weeks it took for ever and when i fruited it i saw trich coming from the bottom ..bleh..

did u pasturize your coir well?!




The jars were quart jars inoculated with ms syringes from trusted vendors. They rocked through the wbs pretty fast. That's part of the reason I'm concerned about the tubs.

I used Damion5050's elementary coir tek to pasteurize the coir. I followed that tek exactly. I even temped it at 45 minutes when I mixed it and it was at about 160F. I think I will just pasteurize in a pot on the stove next time so I can watch the temps better.

EDIT: Did you mean tub size instead of jars?
The tubs are 18 gallon so 72 quart tubs. I spawned 7 quart jars that were 3/4 full so a little more than 5 quarts of bird seed to about 10 quarts of sub for a 1:2 ratio. The sub is 4" deep.

EDIT again:
Quote:

botanisthype said:
High co2 helps incubate well before fruiting...



I just thought of something, might be a dumb idea.
I have a 10 pound co2 tank and regulator. You think I could give them a quick blast through one of the holes and see if they take off? Just to see if the co2 concentration is my problem?

Edited by resonance77 (05/02/10 11:15 AM)

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Invisiblebotanisthype
P$ylocibe$ounDW@vE!
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Registered: 07/31/03
Posts: 839
Loc: DECEPTICON MANOR
Re: tub colonizing slowly [Re: resonance77]
    #12493463 - 05/02/10 12:31 PM (14 years, 8 months ago)

ummm i dunno..i never tried it...but

i think...it could be a temp..problem...what temp. were you incubating your tubs?

the time your stating...sounds like the tubs have been in room temp .like in 60's? so hence theyre a few days behind...try raising it to 80....then fruit in room temp,  as the temp drop. helping it initiate fruiting conditions.


--------------------
Please +REP if i've been of any help... thanks!!!

Dinosaur of the Funny Family, though I have short arms, they fit in my GB... lol

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Invisibleresonance77
Stranger


Registered: 03/01/10
Posts: 103
Re: tub colonizing slowly [Re: botanisthype]
    #12496301 - 05/02/10 10:31 PM (14 years, 8 months ago)

Quote:

botanisthype said:
ummm i dunno..i never tried it...but

i think...it could be a temp..problem...what temp. were you incubating your tubs?

the time your stating...sounds like the tubs have been in room temp .like in 60's? so hence theyre a few days behind...try raising it to 80....then fruit in room temp,  as the temp drop. helping it initiate fruiting conditions.




The tubs have been at 75F most of the time. It has dipped as low as 65F but not for long. I would say 75F for 90% of the time.

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Invisibleresonance77
Stranger


Registered: 03/01/10
Posts: 103
Re: tub colonizing slowly [Re: resonance77]
    #12502105 - 05/03/10 10:29 PM (14 years, 8 months ago)

I'm pretty sure these lids are tight like a tiger. Hasn't anyone else used these Rubbermaid Roughnecks before? I don't think that this shit can breath with all the holes taped tight. I think that the two later tubs should be farther along than 5% by day 9. Everything still looks great, except for the lack of growth. It's not pinning. There are no metabolites. It hasn't completely stalled. There are no signs of any sort of contamination. I'm getting worried. At this rate, the first tub will be done colonizing at about the three week mark and the other two hopefully some time this year. I'm reading all this shit about tubs being colonized in like 10 days, and reading that the longer it takes, the more chance of contamination. I need some advice and guidance with trouble shooting this thing. I am out of ideas, except for the gas exchange. Anyone rocking the Roughnecks out there?

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Offlinetajmahal420
Stranger than some....

Registered: 05/23/09
Posts: 533
Last seen: 2 years, 4 months
Re: tub colonizing slowly [Re: resonance77]
    #12502202 - 05/03/10 10:44 PM (14 years, 8 months ago)

Try loosening the lids just a tad.  You do not want a completely sealed tub.  CO2 is good for colonization, but too much can cause your substrate to stall.  You need to give excess gases an outlet to escape the tub somehow.  The fact that it's not completely stalled harbors signs of hope.

With a spawn ratio of 1:2, those tubs should have been done pretty damn fast.  If it's not GE, then I'm stumped, unless the substrate dried out, but that usually induces premature pinning.  Hmmmmm.

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Invisibleresonance77
Stranger


Registered: 03/01/10
Posts: 103
Re: tub colonizing slowly [Re: tajmahal420]
    #12519883 - 05/06/10 10:57 PM (14 years, 8 months ago)

I took the tubs out of the bags two days ago and replaced the duct tape on the top two holes with micropore tape. It made a noticeable difference over night. I then replaced one of the micropore holes with tightly stuffed polyfill and the shit took off. Probably the biggest one day difference I have seen in myc growth. I'm pretty sure that the problem was GE all along. I hope these tubs make it to first flush. The first tub is on day 20 and is at 90%. The other two tubs are at day 14 and at 20%. But at least they are going now, and everything still looks great. At least, I am prepared for the next round and know how to set my shit up right from the start.

Edited by resonance77 (05/09/10 10:19 PM)

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Invisibleresonance77
Stranger


Registered: 03/01/10
Posts: 103
Re: tub colonizing slowly [Re: resonance77]
    #12534474 - 05/09/10 10:16 PM (14 years, 8 months ago)

Still waiting. The Cambo tub has a few small spots not colonized. It is on day number 23. The LP tub is looking better, I'd guess 50% colonized. It is on day number 17. The PE tub is at about 60% colonized with some killer looking white. It is also on day number 17. Everything still looks 100% healthy. Temps are good (room temp is ~70F). Sub hasn't dried out, I can clearly see it through the Plexiglas top and it is still dark. No bad smell. No pins. No stalling. Just slow as shit and I can't figure out why. As of now, I have one of the two top holes covered with a single layer of micropore tape and the second top hole stuffed tight with polyfill. They seem to be moving along faster than they were, but still very slow. If I restrict the holes any further, it slows down dramatically. I'm afraid to loosen the holes any more and dry the sub out. Just stuck with slow ass tubs, I guess.

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Offlinekentishale
Stranger

Registered: 03/01/10
Posts: 64
Last seen: 14 years, 6 months
Re: tub colonizing slowly [Re: resonance77]
    #12535990 - 05/10/10 09:17 AM (14 years, 8 months ago)

Damn, sounds like my mycobags, they took way longer than I expected when comparing them to other folks notes.... I wish there was a list of factors that can cause slow myc growth....

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Invisibleresonance77
Stranger


Registered: 03/01/10
Posts: 103
Re: tub colonizing slowly [Re: kentishale]
    #12554720 - 05/13/10 11:09 AM (14 years, 8 months ago)

The first tub is on day 26. It hasn't done anything in a couple days. Appears to be stalled. This is the cambo tub. Should I try to fruit it as is?




This is the PE tub on day 20. It's looked like this for a couple days with no noticeable growth as well. Is this one good to go?



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Invisibleresonance77
Stranger


Registered: 03/01/10
Posts: 103
Re: tub colonizing slowly [Re: resonance77]
    #12557645 - 05/13/10 07:51 PM (14 years, 8 months ago)

I went ahead with the PE tub. Still not sure about the Cambodian tub though. Any thoughts on if I should fruit this thing or not. It hasn't moved at all in the last couple days.

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Invisibleresonance77
Stranger


Registered: 03/01/10
Posts: 103
Re: tub colonizing slowly [Re: resonance77]
    #12559378 - 05/13/10 11:56 PM (14 years, 8 months ago)

Quote:

resonance77 said:
... Any thoughts on if I should fruit this thing or not. It hasn't moved at all in the last couple days.




all yall where noobies once too, right? at least tell me off, but please give
me a clue

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Invisible99BOOMERMAN
The Professional
Male


Registered: 06/21/09
Posts: 539
Loc: USA Flag
Re: tub colonizing slowly [Re: resonance77]
    #12559955 - 05/14/10 02:06 AM (14 years, 8 months ago)

Results vary sometimes. From what I have gathered , coir won't colonize in a solid brick in every instance. I have "seen" tubs that look like yours and fruit alright.
  But the solid , fully colonized substrates do fruit better IMHO.However, if you see the mycelium growing up the walls a bit, like looking for substrate to feed on, then you are ready to fruit that bad boy.
  Just wanted to give a hand , since no one jumped in yet.Good luck, and remember , results will vary a Bit depending on conditions(temp,moisture content, G/E, FAE etc.).


--------------------
Anything posted in this thread is just some well thought out bull-shit,which is completely for entertainment purposes only.
                                     
                                      AMU      "Q&A's  Thread"


INGENIOUS MONO TUB LINER

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Invisibledrewb149
Male

Registered: 09/24/09
Posts: 363
Loc: Flag
Re: tub colonizing slowly [Re: 99BOOMERMAN]
    #12561026 - 05/14/10 09:54 AM (14 years, 8 months ago)

fruit it. i like using the wax paper method. and if you can supply constant fae contams wont be any problem. at least for a while i have a tub on its 4 or 5th flush looks brutal but its still good. how was the moisture in your sub when u applied spawn cause if its not field capacity and the surface dries out too much the mycelium will have a more difficult time colonizing it. i ran into that problem with a tub and because spots of the surface took so long to colonize i ended up with trich.

Edited by drewb149 (05/14/10 09:55 AM)

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Invisibleresonance77
Stranger


Registered: 03/01/10
Posts: 103
Re: tub colonizing slowly [Re: drewb149]
    #12572220 - 05/16/10 10:13 AM (14 years, 8 months ago)

Lost the La Premavera tub today. 23 days since spawning and never colonized completely. Checked it this morning and it has a big ugly spot right in the middle of the surface.

The PE tub looks awesome and the Cambo tub is holding on even though it isn't looking great. The strange thing is I prepared all three tubs exactly the same way and kept them in the exact same conditions and I'm getting very different results with each of them. Makes it difficult to deduce the cause of the troubles.

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