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Amazon Shop for: ½ Pint Jars, Agar, Petri Dish, Potato Dextrose, Pressure Cooker

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OfflineMycolaureat
mycophyle

Registered: 01/16/03
Posts: 43
Last seen: 12 years, 3 months
Q's for agar experts
    #1249578 - 01/26/03 08:10 AM (13 years, 10 months ago)

- Which is your best agar recipe for tissue cloning?
- Which kind of yeast work best for you? for wine or for beer?
- What can replace 'para-film' to seal up petri dish's ?
- What's your tricks to prevent condensation in your petri ?
- How do you sterilise petri after use ?

Thanks a lot!


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OfflineAnnoA
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Re: Q's for agar experts [Re: Mycolaureat]
    #1249586 - 01/26/03 08:13 AM (13 years, 10 months ago)

- What can replace 'para-film' to seal up petri dish's ?
Glad wrap.

- What's your tricks to prevent condensation in your petri ?
www.fungifun.org/agar


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OfflineMycolaureat
mycophyle

Registered: 01/16/03
Posts: 43
Last seen: 12 years, 3 months
Re: Q's for agar experts [Re: Anno]
    #1249628 - 01/26/03 08:29 AM (13 years, 10 months ago)

glad wrap!!!
dawm, why i didn't thought about it ..

-i've tried the hot water cup thing and my glove box became filled with condensation... i need another kind of heat source

thanks anno


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OfflineAnnoA
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Re: Q's for agar experts [Re: Mycolaureat]
    #1249638 - 01/26/03 08:34 AM (13 years, 10 months ago)

>i've tried the hot water cup thing
What about a jar with hot water and a lid?


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InvisibleRoadkill
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Re: Q's for agar experts [Re: Mycolaureat]
    #1249648 - 01/26/03 08:39 AM (13 years, 10 months ago)

I like the PDYA best.

Potato Dextrose (Yeast-extract) Agar (PD(Y)A)
broth from boiling 150 grams sliced potatoes
in 500ml water for 30 minutes(add water to 500ml)
(You can use 5 g of instant potato flakes instead)
9 g agar agar
7 grams dextrose( = glucose, or 10 ml honey or corn syrup )
1 gram brewers yeast or yeast-extract (optional)

I make my PDYA like this.
500ml of water.
5 grams of instant potato flakes.
9 grams of agar agar.
7 grams of honey.
1 gram of nutritional yeast.
Pressure cook for 40 minutes.


I use electrical tape instead of para-film.


I use Anno's method to stop condensation on the petri dishes.  :grin:


I wash the petri dishes out really good with beachy water.
Put them in the dishwasher and run that once.
I have a dry-clave tattoo sterilizer so I don't have to use a pressure cooker.
I heat them up at 350 degrees in the dry-clave for 60 minutes.
 


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Who the hell you callin crazy?
You wouldn't know what crazy was if Charles Manson was eating froot loops on your front porch!


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PM the names with on there names, that means they have mushrooms for sale.



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OfflineMycolaureat
mycophyle

Registered: 01/16/03
Posts: 43
Last seen: 12 years, 3 months
Re: Q's for agar experts [Re: Mycolaureat]
    #1249679 - 01/26/03 09:01 AM (13 years, 10 months ago)

Thanks guys!

You rock!!!

peace


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Amazon Shop for: ½ Pint Jars, Agar, Petri Dish, Potato Dextrose, Pressure Cooker

Mushrooms, Mycology and Psychedelics >> Mushroom Cultivation

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