|
Some of these posts are very old and might contain outdated information. You may wish to search for newer posts instead.
|
scatmanrav
Brainy Smurf
Registered: 05/08/04
Posts: 11,483
Loc:
Last seen: 11 years, 2 months
|
Re: TNG thenexgenration isolation techniques w/ no hepaorgbox [Re: fungus_tao]
#12448429 - 04/24/10 09:36 AM (13 years, 11 months ago) |
|
|
Right see, hes trying to put agar in a jar, and sector the agar using a needle through an injection port..not a LC, agar still..but sectoring with needle.
So hes trying to use the 2D medium...just not in a way thats going to allow him to get the sectors out..I think he thinks he can suck a whole agar wedge into his needle.
|
Biffzilla
Emperor of Bongolia
Registered: 08/14/08
Posts: 1,277
Last seen: 11 years, 3 months
|
Re: TNG thenexgenration isolation techniques w/ no hepaorgbox [Re: scatmanrav]
#12448779 - 04/24/10 11:15 AM (13 years, 11 months ago) |
|
|
ANYWAYS here's a great link
http://www.shroomery.org/forums/showflat.php/Number/4947987#4947987
Agar's ghettobag [glovebox substitute]. That's a beaut. Use only one hand as needed tied inside the bag... lol brilliant. so ya fuck this idea... lol i was so obsessed about doing syringe isolates... ====================
...my intention with the tiny jars is to have a flat surface of agar on the bottom of them. only one nib will be nipped off with the tip. then a little bit of water will be squirted into the very shallow jar, which only has medium on the bottom like a petri dish, and suck up the piece that was jabbed off. I DONT THINK I can suck up an entire sector, lol, no, but could chupp off a tiny piece with a thin blunt tipped syringe like a cookie mold, and let it loose in the jar with a tiny squirt of sterile water. I mentioned I wanted to clone a spawn jar's multispore mycelium, but I couldn't get a piece of mycelium sucked up through the syringe wit the water I squirted in. Which is why I thought maybe a thinner needle with a flat tip to make the tinier 'cookie' mold of a section of mycelium, and then having a fatter needle suck that tiny loose bit up that I nipped/ chupped (not chopped) off with the thinner needle. I think chances are it will be sucked up easier than chupping it off with the bigger needle so that the chupped off bit is smaller than the needle's bore, otherwise yes it couldn't readily be sucked up... the fatter one which would be better off sharp, to spare the self healin silicon blob...
but as roger suggests still air agar transfers, I MAY try that as well, its just consistency is what Im after I guess in a very anal retentive way. sounds way easier... i just don't want to waste time and effort, rather do something I know won't contam. I have a glovebox no money for a new one, but its a shit glovebox... i guess for doin agar transfers it DOES make things easier, but that's all I'd do in it, no needles in that thing needing sterilization lol!
but I agree on the still air thing.
and no my intention isnot to such up an entire sector's wedge. only one piece I poke off with the needle tip, someone suggested using a blunt needletip to make a sort of cookie-dough mold, you know. The jar being shallow enough (its a bother where I live to get petri dishes) for the needle to reach the bottom, yes there is a flat 2D surface of medium, off which only a piece of sector will be poked off with the needle. And I would repeat this via the inoculation port until I get single sectors...
but you mention a still air box. would hanging moist bleach solution towels around the work area (sort of curtain it off add in it being a still air area, i mean the wet would make flying particles stick, and the bleach... mmm. the only place I have to work is inside a bath, the bathroom here has a carpet =[ I was thinking of making an island worksurface surrounded by steaming water, creating updrafts to prevent things landing much like the ol' ( think it was shroomgod or shroomking's?) oven tek?
Edited by Biffzilla (04/24/10 01:55 PM)
|
scatmanrav
Brainy Smurf
Registered: 05/08/04
Posts: 11,483
Loc:
Last seen: 11 years, 2 months
|
Re: TNG thenexgenration isolation techniques w/ no hepaorgbox [Re: Biffzilla]
#12449804 - 04/24/10 02:29 PM (13 years, 11 months ago) |
|
|
Once you squirt water into the jar, with the medium at the bottom, the water will wash off pieces from the whole plate, not just a sector..which will continue to go on each time you transfer. All it takes is a few cells, and you cant get an isolate.
All of this idea is exactly a waste of time and effort and something you dont know if it will contaminate or not. If you trying to isolate from bacteria or molds, and you squirt water in, you'll suck up the contamination too.
So if you "just don't want to waste time and effort, rather do something I know won't contam."
Then do what works and whats suggested. Still air glove box or bag, or clean room. There is no 100%..even with a flowhood you can get a contam..but if you do 5 or 10, they wont all contam, theres your guarantee.
|
hisheater
trigger man
Registered: 01/24/09
Posts: 219
Loc: PNW
Last seen: 9 years, 3 months
|
Re: TNG thenexgenration isolation techniques w/ no hepaorgbox [Re: scatmanrav]
#12453444 - 04/25/10 12:35 PM (13 years, 11 months ago) |
|
|
Thanks RR. I was talking about isolating a potential clone, like how you isolate a person to keep them away from everyone. Basically just finding which mush you want to clone and separating it from the rest.
@Biffzilla- yes a clone would save you alot of time and stress. If you isolate you have to run test jars to see which strain you want, because you can't get an isolate from a clone because there are multiple strains in it. So each isolate you get you would have to knock up a test jar to see if it even produces mushrooms, let alone good mushrooms. With cloning you know you'll at least get mushrooms, and the quality/quantity will be higher that MS.
-------------------- They call them fingers but I never see them fing... Oh wait, there they go. ----------------------------------------------- dangermike78 said: "Or are you to ignorant to get that?" The most hilarious post to date... Doo Yoo Hookah???
|
Psuper
Psilocybin
Registered: 02/12/08
Posts: 2,874
Loc: PNW
Last seen: 3 years, 6 months
|
Re: TNG thenexgenration isolation techniques w/ no hepaorgbox [Re: hisheater]
#12453674 - 04/25/10 01:14 PM (13 years, 11 months ago) |
|
|
Scatmanrav, I don't know how you have the patience to read through this thread. Nothing in this thread is interesting or worth reading except your posts. You must occupy the mind state of an advanced Zen master. ~P~
-------------------- Clinical Management of High Dose Psilocybin Sessions: https://www.youtube.com/watch?v=FbHOTIqjZLk
|
CH HELL
Brain Sturgeon
Registered: 10/02/08
Posts: 6,610
Loc: mars
|
Re: TNG thenexgenration isolation techniques w/ no hepaorgbox [Re: Psuper]
#12454185 - 04/25/10 02:31 PM (13 years, 11 months ago) |
|
|
Quote:
Psuper said: Scatmanrav, I don't know how you have the patience to read through this thread. Nothing in this thread is interesting or worth reading except your posts. You must occupy the mind state of an advanced Zen master. ~P~
|
Psuper
Psilocybin
Registered: 02/12/08
Posts: 2,874
Loc: PNW
Last seen: 3 years, 6 months
|
Re: TNG thenexgenration isolation techniques w/ no hepaorgbox [Re: CH HELL]
#12454267 - 04/25/10 02:48 PM (13 years, 11 months ago) |
|
|
Quote:
CH HELL said:
Quote:
Psuper said: Scatmanrav, I don't know how you have the patience to read through this thread. Nothing in this thread is interesting or worth reading except your posts. You must occupy the mind state of an advanced Zen master. ~P~
I got upset just reading the thread title. ~P~
-------------------- Clinical Management of High Dose Psilocybin Sessions: https://www.youtube.com/watch?v=FbHOTIqjZLk
|
CH HELL
Brain Sturgeon
Registered: 10/02/08
Posts: 6,610
Loc: mars
|
Re: TNG thenexgenration isolation techniques w/ no hepaorgbox [Re: Psuper]
#12454319 - 04/25/10 02:59 PM (13 years, 11 months ago) |
|
|
Quote:
Psuper said:
Quote:
CH HELL said:
Quote:
Psuper said: Scatmanrav, I don't know how you have the patience to read through this thread. Nothing in this thread is interesting or worth reading except your posts. You must occupy the mind state of an advanced Zen master. ~P~
I got upset just reading the thread title. ~P~
I totally agree with you PeaSouper. I didn't read any of the long winded post and these are the first actual words I have typed in it.
|
Biffzilla
Emperor of Bongolia
Registered: 08/14/08
Posts: 1,277
Last seen: 11 years, 3 months
|
Re: TNG thenexgenration isolation techniques w/ no hepaorgbox [Re: CH HELL]
#12482716 - 04/30/10 10:34 AM (13 years, 10 months ago) |
|
|
well good for ya lol.
i dont suppose you could do isolates without [even] a glovebox, or figure out a way to?
-------------------- =================================================================== - Show me the honey - ===================================================================
|
scatmanrav
Brainy Smurf
Registered: 05/08/04
Posts: 11,483
Loc:
Last seen: 11 years, 2 months
|
Re: TNG thenexgenration isolation techniques w/ no hepaorgbox [Re: Biffzilla]
#12482882 - 04/30/10 11:22 AM (13 years, 10 months ago) |
|
|
I dont think anyone sees a reason for it. A cardboard box works well, is free, small, and can be recycled. I posted how it could be done though, in a sterilized bathroom.
Pandora's box is not what they claim it is, But what you believe it is I see the Pandora's box as an opening, As a challenge, To see the things you want to hide from yourself, Perspectives run amok when pushed into a corner Up and going down, So sure of ever after, My heart scraped raw, I drink from the well of dreams, Sensibility on a leash, Hallucination on a looser leash So I can play with it, When everything else fails It seems, Things just fall together the way that they want to, Regardless of what one wants or anticipates Maybe this is what's happening here, Maybe we never stopped happening One quarter inch by One quarter inch, Times two, Equals 8 hours of infinite perception You have to love When life comes into perspective, And you can live for the moment
|
Biffzilla
Emperor of Bongolia
Registered: 08/14/08
Posts: 1,277
Last seen: 11 years, 3 months
|
Re: TNG thenexgenration isolation techniques w/ no hepaorgbox [Re: scatmanrav]
#12487434 - 05/01/10 05:29 AM (13 years, 10 months ago) |
|
|
i mentioend in the meantime i found agars ghetto glove bag tek i think ill go that route =] some drugs are bad, mkay, thas all i gotsta say bout this thread of mine. apologies for the cuntliness of it all.
-------------------- =================================================================== - Show me the honey - ===================================================================
|
|