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InvisibleSixTango
Mycota

Registered: 01/21/02
Posts: 1,996
Loc: A little North of Paradis...
Cheap permeant jar lid filter.
    #1247575 - 01/25/03 09:44 AM (18 years, 10 months ago)

Get some plastic lids, like these (or metal) ;


Find some rubber grommets like these;



Drill hole in the lid, that the rubber grommet will fit in tight & insert grommet in lid.

Trim & roll some polyester filter fabric into a tiny roll just a bit bigger than the hole in the grommet & insert it into the hole (tightly). Polyester fabric (about the same as a filter disk) can be found at most large fabric stores (cheap).

Drill another small hole in the lid & insert a polycarb plug (as I did).

Or, use any small stainless steel screw threaded into the hole. As long as the hole is smaller than the screw, it will self thread right into the plastic lid.

The end result is this:


This lid provides a port to inject into & a gas exchange filter that does not require changing each time. As in ready to use - whenever you want without adding any internal filter material.

You can also use this lid for liquid cultures (Karo tek - et. al.). As it provides for gas exchange & has an injection & withdrawal port - all on the lid.

All parts will withstand PC'ing. Cost (not counting lid) is about 0.25 cents each, if you build it -- yourself.

6T  :tongue:   


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~whiskey river rafting, hot tubbing, dirty dancing & spending money on - wild women - having fun & just gonna waste the rest~


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Offlinelvleph
mathematicalidiot

Registered: 11/12/02
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Re: Cheap permeant jar lid filter. [Re: SixTango]
    #1247580 - 01/25/03 09:46 AM (18 years, 10 months ago)

Interesting, but I would fuck it up.


--------------------
"Man was born free and is every where in chains."


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InvisibleSixTango
Mycota

Registered: 01/21/02
Posts: 1,996
Loc: A little North of Paradis...
Re: Cheap permeant jar lid filter. [Re: lvleph]
    #1247639 - 01/25/03 10:15 AM (18 years, 10 months ago)

They are as simple to make as flushing a toilet & almost F/U proof. Once built, they cannot be F/U, unless you intend to.
6T :tongue:


--------------------
~whiskey river rafting, hot tubbing, dirty dancing & spending money on - wild women - having fun & just gonna waste the rest~


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Offlinevatoloco
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Post deleted by MOE THE MAD SCIENTIST [Re: SixTango]
    #1247993 - 01/25/03 01:47 PM (18 years, 10 months ago)



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InvisibleSixTango
Mycota

Registered: 01/21/02
Posts: 1,996
Loc: A little North of Paradis...
Re: Cheap permeant jar lid filter. [Re: vatoloco]
    #1248207 - 01/25/03 03:20 PM (18 years, 10 months ago)

You can also build a Cadillac lid like this, If you can find the parts?

This one has a hepa grade mini filter on it, plus a 3 way valve ( 1. closed 2. air exchange 3. fluid addition or withdrawal).

The treads on the valve match a syringe. Pic below is a quart jar with a 60 ml (2 oz) syringe attached. 6T  :wink: :tongue:
 


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~whiskey river rafting, hot tubbing, dirty dancing & spending money on - wild women - having fun & just gonna waste the rest~


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InvisibleSixTango
Mycota

Registered: 01/21/02
Posts: 1,996
Loc: A little North of Paradis...
Re: Cheap permeant jar lid filter. [Re: SixTango]
    #1248220 - 01/25/03 03:26 PM (18 years, 10 months ago)

Here is the same lid without the syringe. 6T  :wink:
 


--------------------
~whiskey river rafting, hot tubbing, dirty dancing & spending money on - wild women - having fun & just gonna waste the rest~


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InvisibleSixTango
Mycota

Registered: 01/21/02
Posts: 1,996
Loc: A little North of Paradis...
Re: Cheap permeant jar lid filter. [Re: SixTango]
    #1248247 - 01/25/03 03:33 PM (18 years, 10 months ago)

And if you want, you can build a 1 gallon liquid culture jar (include internal stirring rod). 6T  :wink:
 


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~whiskey river rafting, hot tubbing, dirty dancing & spending money on - wild women - having fun & just gonna waste the rest~


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OfflineRobbyrob
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Registered: 12/27/02
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Re: Cheap permeant jar lid filter. [Re: SixTango]
    #1248264 - 01/25/03 03:40 PM (18 years, 10 months ago)

man i just had a hard as time making 4 holes on some jars i can imagine this shit..

ROB


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Offlineshirley knott
not my real name
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Re: Cheap permeant jar lid filter. [Re: SixTango]
    #1248273 - 01/25/03 03:42 PM (18 years, 10 months ago)

yet another 5-shroom 6Tango post.
if you can get the materials and have the skills, this is the way to do it limo-style

:tongue:shirl
xx


--------------------
buh


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InvisiblePinhead
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Re: Cheap permeant jar lid filter. [Re: SixTango]
    #1248284 - 01/25/03 03:46 PM (18 years, 10 months ago)

who sells those plastic lids?


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InvisiblePinhead
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Re: Cheap permeant jar lid filter. [Re: Pinhead]
    #1248345 - 01/25/03 04:07 PM (18 years, 10 months ago)

Quote:

who sells those plastic lids?



Nevermind.. I used the "Search" feature.Sporeworks sells 'em.


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InvisibleSixTango
Mycota

Registered: 01/21/02
Posts: 1,996
Loc: A little North of Paradis...
Re: Cheap permeant jar lid filter. [Re: Pinhead]
    #1248492 - 01/25/03 05:14 PM (18 years, 10 months ago)

Cough..........ummmmmm CLICK HERE:

Something like $2.79 = 8 plastic lids

6T


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~whiskey river rafting, hot tubbing, dirty dancing & spending money on - wild women - having fun & just gonna waste the rest~


Edited by SixTango (01/25/03 05:23 PM)


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InvisiblePinhead
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Re: Cheap permeant jar lid filter. [Re: SixTango]
    #1248582 - 01/25/03 05:43 PM (18 years, 10 months ago)

oh oh..you did a bad thing, sporeworks is a sponser I beleive! But thanks for the link.will save me a buck or two!


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Invisibleboxtop703
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Re: Cheap permeant jar lid filter. [Re: SixTango]
    #1249006 - 01/25/03 09:16 PM (18 years, 10 months ago)

Post deleted by administrator.


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InvisibleRoadkillM
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Re: Cheap permeant jar lid filter. [Re: SixTango]
    #1249080 - 01/25/03 10:37 PM (18 years, 10 months ago)

hi....bro

Any chance you have some links for those parts?
Or tell us where we can get those locally.
I'd like to try making some of those up.  :grin:
Tyvek has been good to me...but to have a permanent lid without having to use some kind of filter disc's is just too fukin cool.
I stopped buying filter disc's from a certain Vendor...why support a jerk when I can make them for free.

 


--------------------
Laterz, Road

Who the hell you callin crazy?
You wouldn't know what crazy was if Charles Manson was eating froot loops on your front porch!


Brainiac said:
PM the names with on there names, that means they have mushrooms for sale.



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OfflineStarspawn
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Re: Cheap permeant jar lid filter. [Re: SixTango]
    #1249378 - 01/26/03 05:33 AM (18 years, 10 months ago)

Hey 6T would you please post the way you put that 1G.liquid jar together.That could be the greatest of all your inventions.I hope your patents are filed,because im sure your concepts would make perfect new products.


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Invisibledog
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Re: Cheap permeant jar lid filter. [Re: SixTango]
    #1249457 - 01/26/03 06:36 AM (18 years, 10 months ago)

Great post. Thanks 6T.  :laugh: 


--------------------


Fascism (fash'izem) n. A governmental system marked by a centralized dictatorship, stringent socioeconomic controls, and often belligerent nationalism. see also: the Bush Administration.


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InvisibleSixTango
Mycota

Registered: 01/21/02
Posts: 1,996
Loc: A little North of Paradis...
Re: Cheap permeant jar lid filter. [Re: Roadkill]
    #1249560 - 01/26/03 07:49 AM (18 years, 10 months ago)

THIS COMPANY

THESE GROMMETS

There are about 2 dozen online companies that sell them. Just do a "google" search with "rubber grommet" as the search term.

You can also find this type rubber grommet at most local electrical supply stores.

6T :tongue: 


--------------------
~whiskey river rafting, hot tubbing, dirty dancing & spending money on - wild women - having fun & just gonna waste the rest~


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InvisibleSixTango
Mycota

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Posts: 1,996
Loc: A little North of Paradis...
Re: Cheap permeant jar lid filter. [Re: Starspawn]
    #1249580 - 01/26/03 08:11 AM (18 years, 10 months ago)

The one gallon liquid culture jar lid was made from 2 brass fittings that you can buy at any hardware store. The stir rod bearing / bushing came from the same company that I linked in this thread. The rod is simple stainless steel - matched to the bearing & pressed in. The filters are medical grade infusion filters, you can find around. Any type high quality small inline air filter will work.

If you want a smaller cheaper fool proof liquid culture container. Just use a good quality baby bottle. You can find them everywhere & cheap


Glass & polycarb ones, plus screw lid & nipple will withstand PC'ing. Insert polyfill in nipple hole, for gas exchange. Squeeze the nipple shut with 2 fingers to protect the polyfill -- so you can agitate the solution, without soaking the polyfil.

You can insert a syringe needle into the nipple & it will reseal when you withdraw it.

Toilet flush simple & available at almost all grocery stores & marts.

6T :tongue: 

EDIT: You should cover the nipple & top 1/4 of the bottle with alc swabbed (allowed to evap) double coffee filters, as a dust cover - held down with a rubber band. Or, you can add them, then PC the whole thing. The coffee filters will dry almost instantly, when you remove the BB from a hot PC. Doing so simply adds an external layer of protection from contams.


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~whiskey river rafting, hot tubbing, dirty dancing & spending money on - wild women - having fun & just gonna waste the rest~


Edited by SixTango (01/26/03 08:34 AM)


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OfflineStarspawn
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Re: Cheap permeant jar lid filter. [Re: SixTango]
    #1249836 - 01/26/03 10:14 AM (18 years, 10 months ago)

Thanks 6T,i thought you had an iv tube in the jar.Is it hooked to a brass pipe?And are those disks a filter?I can see the polyfil in the top so i could'nt understand why you put two of them?My guess would be it needs that much air for the size.The baby bottle was also nice but im ready for a fully loaded model,bells and whistles.What is hooked to the end of the mixing rod,is it a blender type and how much of the bottom does it cover?


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InvisibleSixTango
Mycota

Registered: 01/21/02
Posts: 1,996
Loc: A little North of Paradis...
Re: Cheap permeant jar lid filter. [Re: Starspawn]
    #1249892 - 01/26/03 10:48 AM (18 years, 10 months ago)

The inline filters on  that lid are sterile. The polyfill is just stuck in the tops, to keep them that way.

You simply use a dremel drill tool (or any little drill with a chuck) & tighten it to the external metal rod, then give it a spin, for a few seconds. The end of the rod is flattened a tiny bit, like an oar. It mixes the interior liquid content like a milk shake mixer. Works fantastic.

I use a medical grade hepa filtered air pump.

But, anybody can hook the air line to the "in" filter to any small fish tank air pump, then hook that to a timer, to automate positive pressure air exchange.

6T  :tongue:

Quote:

Thanks 6T,i thought you had an iv tube in the jar.Is it hooked to a brass pipe?And are those disks a filter?I can see the polyfil in the top so i could'nt understand why you put two of them?My guess would be it needs that much air for the size.The baby bottle was also nice but im ready for a fully loaded model,bells and whistles.What is hooked to the end of the mixing rod,is it a blender type and how much of the bottom does it cover?   


 


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~whiskey river rafting, hot tubbing, dirty dancing & spending money on - wild women - having fun & just gonna waste the rest~


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Offlineroygbiv
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Re: Cheap permeant jar lid filter. [Re: SixTango]
    #1249936 - 01/26/03 11:20 AM (18 years, 10 months ago)

excellent info as usual... any suggestions on where to obtain a medicalgrade hepa unit?... just yr standard medical supply outlet?... approximate cost, etc... thanks!



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InvisibleSixTango
Mycota

Registered: 01/21/02
Posts: 1,996
Loc: A little North of Paradis...
Re: Cheap permeant jar lid filter. [Re: roygbiv]
    #1250144 - 01/26/03 01:10 PM (18 years, 10 months ago)

If you know any nurses, med students, EMT's or doctors - they could bag you a few infusion type filters. The larger ones in the 0.22 micron caliber can get spendy. Use google to do a seach on "infusion filters".

You can also convert any number of other filters to this use. Inexpensise in/line gas filters for better model high performance cars work & can be had at any autoparts store for a few bucks - each.

If you are handy & like to tinker? You can build very effective small inexpensive in/line air filters out of PVC pipe & caps. Just use small threaded brass fittings on the caps to create in/out connectors for the air lines.

Use polyfill & tight spunbonded polyester fabric from any FABRIC store (rolled up) as filter material. The polyester filter type fabric is around $2 a yard. Simple to make, very effective & cheap.

6T :tongue:
 


--------------------
~whiskey river rafting, hot tubbing, dirty dancing & spending money on - wild women - having fun & just gonna waste the rest~


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InvisibleSixTango
Mycota

Registered: 01/21/02
Posts: 1,996
Loc: A little North of Paradis...
Re: Cheap permeant jar lid filter. [Re: roygbiv]
    #1250272 - 01/26/03 01:59 PM (18 years, 10 months ago)

There is also another inexpensive way to skin the same liquid culture cat.
You can get a few rubber stoppers w/holes in them at any lab, chem or science supply place. Then get a few glass tubes that fit through the holes in the rubber stoppers (same source). As below:

Then gather up a few quart, half gallon or gallon jugs, as below:

Plug them togather, apply in/line air filters & you ready to do some large volume liquid culture at ghetto prices for the materials. Toilet flush simple.

6T  :tongue:
 


--------------------
~whiskey river rafting, hot tubbing, dirty dancing & spending money on - wild women - having fun & just gonna waste the rest~


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InvisibleSixTango
Mycota

Registered: 01/21/02
Posts: 1,996
Loc: A little North of Paradis...
Re: Cheap permeant jar lid filter. [Re: Starspawn]
    #1250974 - 01/26/03 07:15 PM (18 years, 10 months ago)

Getting back to the subject of this thread. Here is an easy to build lid with a permanent filter installed, as well as an injectable port, that you can injecxt right through & it reseals itself instantly - when you withdraw the syringe needle.
6T  :tongue:
 


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~whiskey river rafting, hot tubbing, dirty dancing & spending money on - wild women - having fun & just gonna waste the rest~


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Invisibleboxtop703
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Re: Cheap permeant jar lid filter. [Re: SixTango]
    #1251010 - 01/26/03 07:29 PM (18 years, 10 months ago)

Post deleted by administrator.


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InvisibleSixTango
Mycota

Registered: 01/21/02
Posts: 1,996
Loc: A little North of Paradis...
Re: Cheap permeant jar lid filter. [Re: boxtop703]
    #1251076 - 01/26/03 08:03 PM (18 years, 10 months ago)

Parts bought in bulk by the K:
Lid around 0.21 cents
plug around 0.2 cents
grommet around 0.7 cents
filter material around 0.1 cent
Labor around 0.16 cents
Total = 0.47 cents each.

6T  :tongue: 


--------------------
~whiskey river rafting, hot tubbing, dirty dancing & spending money on - wild women - having fun & just gonna waste the rest~


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Anonymous

Re: Cheap permeant jar lid filter. [Re: SixTango]
    #1251190 - 01/26/03 08:47 PM (18 years, 10 months ago)

6T,

Where did you get that HEPA pump? WWW?

I'm nobody.


Edited by nobody (01/26/03 10:09 PM)


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InvisibleSixTango
Mycota

Registered: 01/21/02
Posts: 1,996
Loc: A little North of Paradis...
Re: Cheap permeant jar lid filter. [Re: ]
    #1251300 - 01/26/03 09:43 PM (18 years, 10 months ago)

Quote:

6T, Where did you get that HEPA pump?  WW? I'm nobody. 



.............................................

I bought it from "Joe", for a few bucks & it works like a champ. WTF diff does it make where I got it, or how old it is?  :smirk: It serves it's function  :laugh:.

6T  :tongue:

 


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~whiskey river rafting, hot tubbing, dirty dancing & spending money on - wild women - having fun & just gonna waste the rest~


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Anonymous

Re: Cheap permeant jar lid filter. [Re: SixTango]
    #1251327 - 01/26/03 09:51 PM (18 years, 10 months ago)

My friend's monkey was currious. He was kind of wanting one.

I'm nobody.


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OfflineShrewDigsby
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Re: Cheap permeant jar lid filter. [Re: SixTango]
    #1251339 - 01/26/03 09:55 PM (18 years, 10 months ago)

I've read too many posts to not give 6T his 5 deserved shrooms...Like you need them :tongue: 


--------------------
Marijuana is a horticultural plant.  Hemp is an industrial weed.  I believe they were both provided to us by GOD to use and enjoy.


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InvisibleSixTango
Mycota

Registered: 01/21/02
Posts: 1,996
Loc: A little North of Paradis...
Re: Cheap permeant jar lid filter. [Re: ]
    #1251367 - 01/26/03 10:06 PM (18 years, 10 months ago)

Watch Fed & State gov, plus Collage surplus lab eqt auctions & sales. You can find all sorts of lab stuff -- CHEAP. 6T  :tongue:


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~whiskey river rafting, hot tubbing, dirty dancing & spending money on - wild women - having fun & just gonna waste the rest~


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InvisibleSixTango
Mycota

Registered: 01/21/02
Posts: 1,996
Loc: A little North of Paradis...
Re: Cheap permeant jar lid filter. [Re: ShrewDigsby]
    #1251373 - 01/26/03 10:09 PM (18 years, 10 months ago)


:shocked: :laugh: :wink: :tongue: 6T 


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~whiskey river rafting, hot tubbing, dirty dancing & spending money on - wild women - having fun & just gonna waste the rest~


Edited by SixTango (01/26/03 10:20 PM)


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InvisibleSixTango
Mycota

Registered: 01/21/02
Posts: 1,996
Loc: A little North of Paradis...
Re: Cheap permeant jar lid filter. [Re: ShrewDigsby]
    #1253376 - 01/27/03 01:39 PM (18 years, 10 months ago)

:wink: Thanks. Sometimes I need all the help I can get.  :wink:

Forgot to add:

Some of the rubber bumpers will work as injectable septum plugs. Cheap, too.

CLICK HERE:

THE WHOLE CATALOG

6T  :tongue: :wink:
 


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~whiskey river rafting, hot tubbing, dirty dancing & spending money on - wild women - having fun & just gonna waste the rest~


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OfflineNumba9
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Re: Cheap permeant jar lid filter. [Re: SixTango]
    #1253714 - 01/27/03 03:34 PM (18 years, 10 months ago)

Hate to be nit-picky.. but on that True Value link those are storage lids and right at the bottom of that picture of the lids it says specifically..."Not for processing"


--------------------
Remember..what the Door Mouse said..."Feed your Head".......... Jefferson Airplane


Edited by Numba9 (01/27/03 03:38 PM)


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Offlineshroomologist
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Re: Cheap permeant jar lid filter. [Re: Numba9]
    #1253727 - 01/27/03 03:39 PM (18 years, 10 months ago)

they aren't for actually pressure sealing the jars. If you don't put a hole in them, the jars are gonna burst. If you seal them tight, they'll break either themselves or the jar. They're made for sealing air tight with no pressure difference from inside to outside. BUT they are high heat tolerant.


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InvisibleSixTango
Mycota

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Posts: 1,996
Loc: A little North of Paradis...
Re: Cheap permeant jar lid filter. [Re: Numba9]
    #1253750 - 01/27/03 03:48 PM (18 years, 10 months ago)

Quote:

Hate to be nit-picky.. but  those are  storage lids and right at the bottom of that picture of the lids it says specifically..."Not for processing" 




Wow................... :ooo:

You should have told every vendor that sells them -- here, every myco vendor on the web & P. Stamets that. Because they work. Amoungst the small circle of peeps I know nationwide, I would speculate 10's of thousands of them have been repeatedly PC'ed on spawn jars, with nada problemo.



6T  :wink:

EDIT -- I did not mean to sound like a condescending A/H. They work, for this purpose - perfectly. Having PC'ed 20 quarts at a time -- all with these lids, time & time again -- in an AA 941 -- I know they work. WBS is bird seed & it works as mushroom substrate spawn spawn. Cow & horse shit is fecal matter & works as a substrate. Just go with whatever works -- for you & be happy.  :wink: :smile: :tongue: 6T


 


--------------------
~whiskey river rafting, hot tubbing, dirty dancing & spending money on - wild women - having fun & just gonna waste the rest~


Edited by SixTango (01/27/03 05:10 PM)


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InvisibleRoadkillM
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Re: Cheap permeant jar lid filter. [Re: Numba9]
    #1254005 - 01/27/03 05:21 PM (18 years, 10 months ago)

Numba,

I have been using plastic lids for a long time now.
They are fantastic...no rust...and no rust stains on the filter disc's.

Speaking of filter disc's...these will become a thing of the past.

Plastic lids with a permanent filter installed and an injectable port for inserting the needle from a spore syringe is the FUTURE...what a great dirrection to go!
I'm so excited about all of this...no more wasted money on a bunch of filter disc's that only last a few times.
And less chances of getting contams!

6T...thanks bro!



--------------------
Laterz, Road

Who the hell you callin crazy?
You wouldn't know what crazy was if Charles Manson was eating froot loops on your front porch!


Brainiac said:
PM the names with on there names, that means they have mushrooms for sale.



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InvisibleSixTango
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Re: Cheap permeant jar lid filter. [Re: Roadkill]
    #1254155 - 01/27/03 06:24 PM (18 years, 10 months ago)

Shitzzzz

Cats outa the bag --anyway. If you want to buy filter material -- in bulk. There are companies like this all over America & the Internet.

CLICK HERE

That have it on mile long bolts. The cost being about 1 cent each for the same stuff.

But, hell with changing them. Ya got lids. Just install a 0.02 cent perminant filter & that is the end of it -- anyway.

6T


--------------------
~whiskey river rafting, hot tubbing, dirty dancing & spending money on - wild women - having fun & just gonna waste the rest~


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InvisibleSixTango
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Re: Cheap permeant jar lid filter. [Re: Roadkill]
    #1255363 - 01/28/03 12:14 PM (18 years, 10 months ago)



--------------------
~whiskey river rafting, hot tubbing, dirty dancing & spending money on - wild women - having fun & just gonna waste the rest~


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Invisibleboxtop703
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Re: Cheap permeant jar lid filter. [Re: SixTango]
    #1255478 - 01/28/03 12:47 PM (18 years, 10 months ago)

Post deleted by administrator.


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Offlineshroomologist
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Re: Cheap permeant jar lid filter. [Re: SixTango]
    #1255517 - 01/28/03 01:04 PM (18 years, 10 months ago)

here's an even better price

surplus caps for $2 per 24

http://www.labwarehouse.com/lw/recommend.cfm?recommendid=4846&jump=category%2Ecfm%3Fcategory%3D50

1/2 way down the page

they also have gloves, bio suits, glassware, petri dishes, vials and filters

check this filter out, its a fiberglass filterfor air, maybe u can use it for air bubblers inline with the setup, thought i'd post it too.

http://www.labwarehouse.com/lw/recommend.cfm?recommendid=4687&jump=category%2Ecfm%3Fcategory%3D49


Edited by shroomologist (01/28/03 01:12 PM)


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InvisibleSixCee
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Re: Cheap permeant jar lid filter. [Re: shroomologist]
    #1255605 - 01/28/03 01:36 PM (18 years, 10 months ago)

Lab Warehouse is great. There sister company, www.sciplus.com is great too.


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OfflinemotamanM
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Re: Cheap permeant jar lid filter. [Re: SixTango]
    #1255750 - 01/28/03 02:42 PM (18 years, 10 months ago)

6T Lid TEK....


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http://heffter.org


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Offlinedebianlinux
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Re: Cheap permeant jar lid filter. [Re: boxtop703]
    #1257335 - 01/29/03 04:37 AM (18 years, 10 months ago)

these don't appear to be good very long-term use for syringes. I am in need of self-sealing ports that can withstand a whole bunch of punctures. The ports on micronmagick's myco-starters are a good example. they withstand ~50-60 punctures before I don't trust them anymore. myco starters run about 5 bux a pop too. I've had success using RTV silicone sealant but that is really only good for about 5 or six sticks. these that you guys are linking to don't seem to have a "long" enough chunk of rubber to push the needle through before it comes out the underside. correct me if I'm wrong. I'd love to find a good solution.


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Offlineshroomologist
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Re: Cheap permeant jar lid filter. [Re: debianlinux]
    #1257615 - 01/29/03 07:23 AM (18 years, 10 months ago)

those ones i linked are MADE for syringes. When you get a shot, for innoculation at the doctor, he draws a little bit of the serum out of this vial, with that rubber seal on top. Then, puts it back in the storage area (most times a fridge) for use on another patient. They routinely do this, because one vial can innoculate like 10 people. SO its self healing, and its made for a decent amount of punctures. This seal is DESIGNED for multiple punctures with a syringe needle.

Even if you puncture it 100 times, its still gonna seal air tight!, no germs are gonna get through that barrier. They're more likely to come from the polyfil seal.

Just get like 100 of them (~$8) and replace them every 10 batches. Thats more than enough maintenance. Its cheap, and easier than puncturing through a lid, or leaving the hole on the metal lids open to contams after innoculation. By far the best idea i've seen yet. I'm working on a more dependable idea for the filter on the lid, looking at vendors for certain parts that can be used with a filter disk type of setup.


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InvisibleMicronMagick
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Re: Cheap permeant jar lid filter. [Re: debianlinux]
    #1257617 - 01/29/03 07:23 AM (18 years, 10 months ago)

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InvisibleSixTango
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Re: Cheap permeant jar lid filter. [Re: debianlinux]
    #1257644 - 01/29/03 07:40 AM (18 years, 10 months ago)

The ports are designed for "serum" vials or bottles, under strict FDA guidlines & rules. Consiquently, you cannot get a better product, for the purpose. As serum ports are made to withstand repeated syringe needle punctures.

I have no idea of the spec's of MM's ports. Certainly, some are better than -- others. The bigger & better they are, the more money they cost. Some are rubber, ptfe/rubbe, rubber/silicone & some have a teflon lining.

I have differing kinds. As an example, here is a lid I made:


With a port similar to these: CLICK HERE

They work GREAT & will will withstand as many needle punctures, as any -- out there.

If you want to go past 50 or 60 injections, simply pop the port out & replace it with another. Simple as that & MUCH cheaper than buying another lid w/port already installed.

6T :tongue:

 


--------------------
~whiskey river rafting, hot tubbing, dirty dancing & spending money on - wild women - having fun & just gonna waste the rest~


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Offlinedebianlinux
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Re: Cheap permeant jar lid filter. [Re: shroomologist]
    #1257665 - 01/29/03 07:48 AM (18 years, 10 months ago)

thank you both (shroomologist and MM) for your input.


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InvisibleSixTango
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Re: Cheap permeant jar lid filter. [Re: shroomologist]
    #1257798 - 01/29/03 08:29 AM (18 years, 10 months ago)

Certainly, any fiber filter can absorb a liquid solution from the container it is protecting. But, the bulk of the ones I designed are used for innoc of a WBS spawn jar. There is very little possibility of the filter wicking moisture from that, through it to open air. In fact, much less than using a normal filter type disk.

As for liquid culture solutions, as in MM style lid. It will not leak, but -- one cannot provide gas exchange through it. Unless you pierce it with a needle & actually inject air. Excepting, there is no outlet & pressure can build, inside the container.

To resolve that issue, I designed this little puppy:


Which has a 3 way valve ... 1 closed, 2; open to hepa grade filter & 3; open to injection or withdrawal via a syringe.

A tight fitting rubber grommet is placed into a hole drilled in the lid. Then this valve w/filter is inserted tightly in into the grommet. Resulting in an air/liquid tight lid, you can provide gas exchange to, close & agitated without getting the filter wet, and inject or withdraw from, controlled by the valve stem position.

Just tinkering. 6T


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~whiskey river rafting, hot tubbing, dirty dancing & spending money on - wild women - having fun & just gonna waste the rest~


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Offlineroygbiv
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Re: Cheap permeant jar lid filter. [Re: SixTango]
    #1257887 - 01/29/03 08:56 AM (18 years, 10 months ago)

...thanks very much sixtango for all the information... ive got everything i need now...


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InvisibleSixTango
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Re: Cheap permeant jar lid filter. [Re: roygbiv]
    #1257926 - 01/29/03 09:09 AM (18 years, 10 months ago)

There are still a few changes that can be made. One can simply cut tyvek into a small circle (about nickle coin sized), then place it over a rubber grommet, then force the groommet through a tight hole on the lid, from underneath. The grommet holds the tyvek in place on top, perminantly (or until you want to replace it).

6T





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~whiskey river rafting, hot tubbing, dirty dancing & spending money on - wild women - having fun & just gonna waste the rest~


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Offlineshroomologist
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Re: Cheap permeant jar lid filter. [Re: SixTango]
    #1258355 - 01/29/03 11:31 AM (18 years, 10 months ago)

what about this sixtango

If you took a 1 inch grommet, and a 1 inch tube, and cut the tube like 1/2 inch, and got a 1.5 inch piece of filter disk, and put it over the end of the tube, and forced it through the grommet, to create a removable, serviceable filter.

lemme know what u think


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