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Amazon Shop: Brown Rice Flour, Laminar Flow Hood, Oyster Shell, pH Test Strips, Vermiculite

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Invisibledefiningsound
lurker
Registered: 05/14/02
Posts: 8
using microwave to sterilize
    #1244338 - 01/23/03 10:26 PM (14 years, 4 months ago)

Just over two weeks ago I mixed together a substrate with the following components:

2000 mL vermiculite
750 mL water
750 mL organic brown rice flour
2 shot glasses yeast extract (for B vitamins)
2 shot glasses dextrose (for growth energy)
2 shot glasses oyster shell (I should have used aquarium buffer)
2 shot glasses dilute phospohoric acid pH ~5 (I should have used bonemeal)

Psilocibin is pretty much identical to the unstable psilocin with an additional phosphate group attached. I have read a study that showed % psilocin content increasing with each flush. I believe that as there is less available phosphate, less psilocibin is produced. I wanted to provide the fruit with some available phosphate, so that the dried fruit would store longer (psilocin is less stable in air).
Bonemeal has lots of goodies like tryptophan and glycine, I should have used it as a source of phosphate rather than phosphoric acid.

To continue.

I put the substrate into an opaque rubbermaid bin, and microwaved it 10 mins at a time, 6 times. So the wet substrate inside was pretty hot for an hour, and I was hoping that the bad things were dead.

I took a few spore prints and mixed them with a few syringes of water, and sprayed a syringe all over the top of each cooled substrate, in an "oven tek flow hood".

I got impatient (yes, I know patience is the most important part of growing) and opened the bins. Pictures follow:

The first bin is growing quite well! I love the mycelium blasting up the sides of the bin. Very aggressive. There are some greenish areas beneath the mycelium, but my money is on the mycelium.


This bin is almost as strong. Some edges are not completely filled.


I ran out of vermiculite for this bin, so I substituted some straw. It looks pretty lame. To tell you the truth, I am *not* impressed with straw. Sure, it doesn't contaminate easily, but that's because it has almost zero nutrients. I have come back to the PF tek (albeit modified to fit in a bin), because it rocks. My attempts at straw teks have produced massive massive fruits, for one flush. Give me a break, I'm not going to do all kinds of work for one flush.


This one shows zero growth, and absolutely reeks of bacteria. Rotting apples is such an understatement. It is like rotting apples, spiced with the finest ass and feet one can find. I am quite surprised that there was no growth at all. Maybe I was just lucky with the two that worked well.


Does anyone have questions or comments? My plan is to put a casing layer of vermiculite on top, and fruit in the same containers. Air exchange once a day if I can remember to.


--------------------
Despite some very good movies to the contrary, a casino is *not* a good place to be, while on drugs.


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InvisibleRoadkill
Retired Shroomery Mod
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Registered: 12/11/01
Posts: 22,598
Loc: Snoqualmie, Wa.
Trusted Cultivator
Re: using microwave to sterilize [Re: definingsound]
    #1245728 - 01/24/03 11:39 AM (14 years, 4 months ago)

Quote:

The first bin is growing quite well! I love the mycelium blasting up the sides of the bin. Very aggressive. There are some greenish areas beneath the mycelium, but my money is on the mycelium.



My money is on the GREEN mold winning.

good luck!


--------------------
Laterz, Road

Who the hell you callin crazy?
You wouldn't know what crazy was if Charles Manson was eating froot loops on your front porch!


Brainiac said:
PM the names with on there names, that means they have mushrooms for sale.



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Invisibledefiningsound
lurker
Registered: 05/14/02
Posts: 8
Re: using microwave to sterilize [Re: Roadkill]
    #1316244 - 02/18/03 09:13 AM (14 years, 4 months ago)

Roadkill: I have read many of your posts. You were mostly correct. I have given up on this attempt, as all the containers were heavily infected with some sort of bacteria, as well as some yeast perhaps. The green mould never did show up though.

I believe that the massive contamination was because the rubbermaid container had a very poor seal around the lid. As the container got warmer and cooler, the container "breathed" trough the unfiltered loose seal, sucking in all sorts of new flora? fauna? Also, the substrate was too wet.


--------------------
Despite some very good movies to the contrary, a casino is *not* a good place to be, while on drugs.


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InvisibleRoadkill
Retired Shroomery Mod
Male User Gallery

Registered: 12/11/01
Posts: 22,598
Loc: Snoqualmie, Wa.
Trusted Cultivator
Re: using microwave to sterilize [Re: definingsound]
    #1316651 - 02/18/03 12:21 PM (14 years, 4 months ago)

Quote:

Also, the substrate was too wet.



That is one of the main reasons your friend got contams IMHO.
Try putting a drain plug in the bottom side of the container so you can drain excessive water out.
Probably got bacterial contams.

Sorry your friend lost his casings.


--------------------
Laterz, Road

Who the hell you callin crazy?
You wouldn't know what crazy was if Charles Manson was eating froot loops on your front porch!


Brainiac said:
PM the names with on there names, that means they have mushrooms for sale.



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Amazon Shop: Brown Rice Flour, Laminar Flow Hood, Oyster Shell, pH Test Strips, Vermiculite

Mushrooms, Mycology and Psychedelics >> Mushroom Cultivation

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