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Amazon Shop for: ½ Pint Jars, Brown Rice Flour, Vermiculite

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InvisibleSixCee
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Isopropyl Sterilization
    #1239473 - 01/22/03 11:58 AM (14 years, 3 months ago)

Well I was reading the freeze sterilization post and an idea came to me...

Do you think it could be possible to load up your substrate into jars substituting water with Isoprpyl, letting it evaporate, and then injecting sterile water?

I know it would take a very long time and is not even worth it but do you think it would work?


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Offlinevatoloco
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Post deleted by MOE THE MAD SCIENTIST [Re: SixCee]
    #1239499 - 01/22/03 12:05 PM (14 years, 3 months ago)



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InvisibleSixCee
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Re: Isopropyl Sterilization [Re: vatoloco]
    #1239506 - 01/22/03 12:07 PM (14 years, 3 months ago)

Are you being sarcastic or does the great Vatoloco actually have faith in me!? :laugh:


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-->The above statements may or not be true.
->Quote of the Moment :
"Yea. All bitches are whores who love sex." -Cubie
----> PMs checked daily.


Edited by SixCee (01/22/03 12:47 PM)


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OfflineQuick Blaze
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Re: Isopropyl Sterilization [Re: SixCee]
    #1239539 - 01/22/03 12:17 PM (14 years, 3 months ago)

try it out..who knows it might just work ...if you dont i will in the next couple of weeks becuase this sounds like it could work...


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"Life is one BIG road with Lots of signs. So when you riding through the ruts, don't you complicate your mind. Flee from hate, mischief and jealousy. Don't bury your thoughts, put your vision to reality "


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OfflineX.O
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Post deleted by Moe Howard [Re: SixCee]
    #1239616 - 01/22/03 12:37 PM (14 years, 3 months ago)



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OfflineViking
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Re: Isopropyl Sterilization [Re: X.O]
    #1239638 - 01/22/03 12:44 PM (14 years, 3 months ago)


Since vermiculite is porous, I doubt all the alcohol would ever evaporate.

Viking


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InvisibleSixCee
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Re: Isopropyl Sterilization [Re: X.O]
    #1239643 - 01/22/03 12:45 PM (14 years, 3 months ago)

Im outta spores. So the earliest I can try it is if my current grow is successfull and I make a print or it fails and I buy some more. Either way it will be a while.

Hey thanks for the kind words all.


--------------------
~~~~~~~~~~~~~~~~~~~~~~~~
-->The above statements may or not be true.
->Quote of the Moment :
"Yea. All bitches are whores who love sex." -Cubie
----> PMs checked daily.


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InvisibleSixCee
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Re: Isopropyl Sterilization [Re: SixCee]
    #1239651 - 01/22/03 12:48 PM (14 years, 3 months ago)

Then dont use vermiculite. :smile:


--------------------
~~~~~~~~~~~~~~~~~~~~~~~~
-->The above statements may or not be true.
->Quote of the Moment :
"Yea. All bitches are whores who love sex." -Cubie
----> PMs checked daily.


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OfflineAngry Mycologist
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Re: Isopropyl Sterilization [Re: SixCee]
    #1239756 - 01/22/03 01:20 PM (14 years, 3 months ago)

You can be the guinea pig 6C :smile:  Tell us what happens. 


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The proper penalty of ignorance, which is of course that those who don't know should learn from those who do... - Plato


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OfflineLoop_Theorist
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Re: Isopropyl Sterilization [Re: SixCee]
    #1239786 - 01/22/03 01:31 PM (14 years, 3 months ago)

if you're taking grain, then no, I dont think it will kill all of the endospores that grain harbours.

if your talking BRF/verm. jars, then I think it would take days or weeks for all the Iso to evaporate, thus slowing you down a LOT.



-Loop


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Pack-tight, midnight, ship it to tha coast.
Some take tha plane, you, you on tha boat,
right beside tha money which right beside tha coke
Passin by tha coastgaurd, right under their nose.

To the US-HEY where anyone can getny, MIA all the way to New Your city, you a twenty now....


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InvisibleSixCee
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Re: Isopropyl Sterilization [Re: Loop_Theorist]
    #1240334 - 01/22/03 04:42 PM (14 years, 3 months ago)

I dont think it will take weeks for it to all evaporate, well two weeks at maximum.

I too dont think that this will work for grains but maybe it will I dunno. The only problem I see with this is thinking that all of the isopropyl is evaporated when it is not and innoculating preventing the spores from germinating.

Ok I will test this out as soon as I get some spores. I see some mycelium growth in my jars so I should be able to at least get an isolate.


--------------------
~~~~~~~~~~~~~~~~~~~~~~~~
-->The above statements may or not be true.
->Quote of the Moment :
"Yea. All bitches are whores who love sex." -Cubie
----> PMs checked daily.


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Amazon Shop for: ½ Pint Jars, Brown Rice Flour, Vermiculite

Mushrooms, Mycology and Psychedelics >> Mushroom Cultivation

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