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OfflineLegoulash
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Freeze sterilization?????
    #1239041 - 01/22/03 09:33 AM (13 years, 10 months ago)

My friend thought about this today. Would freezing your substrate kill bacteria and other contam problems? Like if i were to freeze Millet or BRF for a couple days already prepared in jars, then let them cool off in a sterile oven or something. Then do a normal PCing or boiling?


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Offlineshroominbloom
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Registered: 11/18/02
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Re: Freeze sterilization????? [Re: Legoulash]
    #1239066 - 01/22/03 09:44 AM (13 years, 10 months ago)

If you're going to PC, then there really isn't any reason to do anything else. As long as you PC for 60 mins, you should be just fine. I think that bacteria (and other contams) are able to survive cold temperatures the same way they are (sometimes) able to survive boiling: endospores. This is what they use in nature, when the temperature drops below freezing (or to 7*F like it is where I am right now).


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OfflineCurious_George
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Re: Freeze sterilization????? [Re: shroominbloom]
    #1239106 - 01/22/03 09:56 AM (13 years, 10 months ago)

If you thaw out a steak and leave it on the counter for a week.. will it stink!?

You would need like -xxx degrees to do something like that,, and even then.. i don't think it would work anyways!!

nice thought, but nope! sorry.

cg


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OfflineSHR00M0L0GIST
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Re: Freeze sterilization????? [Re: Legoulash]
    #1239170 - 01/22/03 10:22 AM (13 years, 10 months ago)

Reply to:
Quote:

if i were to freeze Millet or BRF for a couple days already prepared in jars, then let them cool off in a sterile oven or something




If you freeze something, how are you gonna let it "cool off" in an oven? LOL j/k


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Offlinekb73
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Re: Freeze sterilization????? [Re: Legoulash]
    #1239171 - 01/22/03 10:22 AM (13 years, 10 months ago)

I don't think so. Freezing just slows down the growth of bacteria. The colder it is, the slower they grow. That's why a freezer keeps food longer than a fridge. And eventually, you have to throw things out in the freezer because they will still go bad, it just takes longer.


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InvisibleSixCee
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Re: Freeze sterilization????? [Re: kb73]
    #1239411 - 01/22/03 11:41 AM (13 years, 10 months ago)

I think if you have substrate in the freezer for weeks it may work. Of course a steak will smell if you lieave it out, you could PC it and leave it out and it will smell.


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Offlinemetalchimp
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Re: Freeze sterilization????? [Re: SixCee]
    #1239424 - 01/22/03 11:46 AM (13 years, 10 months ago)

as far as I am aware freezing doesn't kill bacteria it just suspends it's animation as soon as you warm it up the bacteria will resume its business!


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InvisibleSixCee
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Re: Freeze sterilization????? [Re: metalchimp]
    #1239444 - 01/22/03 11:51 AM (13 years, 10 months ago)

So maybe if you seep your substrate in water for a day or so and then freeze it might work.


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Offlineflow
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Re: Freeze sterilization????? [Re: Legoulash]
    #1239453 - 01/22/03 11:53 AM (13 years, 10 months ago)

i think you'd need a very cold freezer for this to work


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Anonymous

Re: Freeze sterilization????? [Re: Legoulash]
    #1239456 - 01/22/03 11:53 AM (13 years, 10 months ago)

unless you have some liquid nitrogen or something like that, but a normal household freezer wouldnt work

and im also wondering how your gonna cool off some frozen substrate. please expound


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OfflineAnnoA
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Re: Freeze sterilization????? [Re: Legoulash]
    #1239581 - 01/22/03 12:29 PM (13 years, 10 months ago)

Freezing doesn?t eliminate bacteria, no.


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