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Offlineupupup
guardian

Registered: 08/25/01
Posts: 889
Loc: George "I love Hitler" Bu...
Last seen: 20 years, 1 month
Unable to get rhyzo growth on agar...... *DELETED*
    #1238674 - 01/22/03 05:04 AM (21 years, 3 months ago)

Post deleted by upupup


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Support bacteria - they're the only culture some people have.

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OfflineRaadt
nicht

Registered: 06/07/02
Posts: 2,107
Loc: azurescending
Last seen: 4 years, 11 months
Re: Unable to get rhyzo growth on agar...... [Re: upupup]
    #1238742 - 01/22/03 05:27 AM (21 years, 3 months ago)

keep it going, i guess. I usually get rhizo's in about 3-4 transfers.


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Raadt

-- The information I provide is only information from readings, growing of gourmet mushrooms, and second hand stories--

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OfflineOn_the_Down-Low
Polyfil-ophile-Say it 3 timesfast.

Registered: 10/15/02
Posts: 401
Last seen: 19 years, 2 months
Re: Unable to get rhyzo growth on agar...... [Re: upupup]
    #1238805 - 01/22/03 05:53 AM (21 years, 3 months ago)

What about agar to grain, and then start over with a spore from that fruiting? Back to the agar , and try reselecting.

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InvisibleChampion des Champignons
long standing member;)

Registered: 07/26/00
Posts: 2,680
Loc: Alba
Re: Unable to get rhyzo growth on agar...... [Re: upupup]
    #1239359 - 01/22/03 09:20 AM (21 years, 3 months ago)

if even cloned tissue from a healthy fruit is not growing rhizomorphically then it's likely that the medium is the problem. I find adding a bit of yeast always helps mycelium grow rhizomorphically.


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hmmm........

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OfflineMycolaureat
mycophyle

Registered: 01/16/03
Posts: 43
Last seen: 19 years, 8 months
Re: Unable to get rhyzo growth on agar...... [Re: Champion des Champignons]
    #1239816 - 01/22/03 11:43 AM (21 years, 3 months ago)

I never had rhyzo growth from a multispores innoculation.
But when i innoc with a mushroom tissue i always get nice rhyzo mycelium (even without yeast.)


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InvisibleZen Peddler
Male User Gallery

Registered: 06/18/01
Posts: 6,379
Loc: orbit
Re: Unable to get rhyzo growth on agar...... [Re: Mycolaureat]
    #1240962 - 01/22/03 06:29 PM (21 years, 3 months ago)

Really? I get constant rhizomorphic mycelia from my original multispore plates - and if i dont by the third transfer I use a different germination point. Go back to your original plate and try with a different germination point, transfer that and see if you get rhizos then. Are the plates still sectoring or is the mycelia all consistant. As long as its sectoring rhizos should be a possibility around the corner.
To be honest, some mushrooms perform better from linear. As an example ive been isolating five different spore-matches of Argent cubies and letting the plate fruit to ensure fruiting genetics. The most rhizomorphic mycelia hasnt fruited as well in this case as the linear stuff.
Another example is pan cyans. Ive found that faster linear mycelia isolated from multispore tends to fruit very poorly, while the sluggish cottony stuff does well often.
Clones are just clones - there shouldnt be a difference in their performance. The idea that cloning nice mushrooms should produce the same rhizomorphic mycelia and strong fruiting is redundant. It often doesnt work out that way.
Second generation and rhizo sectoring:

Pan cyan cottony mycelia fruiting off agar:


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Invisibleblahblahblah
Mad Scientist

Registered: 10/24/01
Posts: 1,022
Loc: South America
Re: Unable to get rhyzo growth on agar...... [Re: upupup]
    #1241000 - 01/22/03 06:50 PM (21 years, 3 months ago)

I would also suggest adding some yeast and also making sure that you are not pressure cooking the your agar too long as the sugars will carmelize resulting in fluffy growth (from what I hear anyway :grin:)

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OfflineMycolaureat
mycophyle

Registered: 01/16/03
Posts: 43
Last seen: 19 years, 8 months
Re: Unable to get rhyzo growth on agar...... [Re: Zen Peddler]
    #1242086 - 01/23/03 05:58 AM (21 years, 3 months ago)

hey bleuemeanie,
Which agar recipe are you using ?

thanks



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InvisibleAlkaloids
3,4,5-trimethoxyphenethylamine
Male

Registered: 11/15/98
Posts: 743
Loc: pubis mons
Re: Unable to get rhyzo growth on agar...... [Re: Mycolaureat]
    #1244060 - 01/23/03 06:06 PM (21 years, 3 months ago)

that's really odd. I only have one print (a B+ print) that I never get rhizo's from , but it still throws off fruit like mad.
I agree that rhizo's can be good, but as Bluemeanie pointed out some strains do well to excellent without ever having rhizo growth.
On that note I have had isolates on agar be finely linear and then get rhizo growth on the final medium.
So listen to what everyone has suggested, but keep testing your isolates, rhizo or not.
good luck


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InvisibleZen Peddler
Male User Gallery

Registered: 06/18/01
Posts: 6,379
Loc: orbit
Re: Unable to get rhyzo growth on agar...... [Re: Alkaloids]
    #1244498 - 01/23/03 10:33 PM (21 years, 3 months ago)

Exactly. If your growing out cubies - test the plates anyway for fruiting performance (with cubies it just takes a bit of warmth, light and a colonised plate) even if there arent any rhizos.
I just use standard MEA


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