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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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Offlinezer0vega
stealing happyhours

Registered: 01/02/03
Posts: 307
Loc: In a van down by the rive...
Last seen: 11 years, 2 months
B+ Growth
    #1235020 - 01/20/03 06:55 PM (22 years, 2 months ago)

i've done my best to research this topic beforehand
about 10 days ago i started to PF tek jars and followed the tek closely, they were steam sterilized very well, and then inoculated with a B+ spore syringe from sporeworks.com, since then they've been in my rubbermaid incubator at about 84 F.

after a few days, they showed signs of growth, and over the next few days showed steady colonization, but in the past couple days i've noticed that the growth has slowed extremely, or has stopped all together, some of the growths(from inoc. points) are about the size of a quarter and display a very slight tan hue, others are whiter and about a half dollar size.. i've also noticed condensation on the sides of the jar.
My questions are, should growth happen in spurts? or should it be steady? and would it seem my jars have a problem? ( i will, of course, give it more time) it would seem that since conditions are fixed, growth should happen at a very steady rate..

also, (i've searched for this as well, but with mediocre results) to save posting another topic, what do you recommend to remedy stalled jars? i know of trying to inject sterile water in the case of dehydrated cakes.. but what are some other possibiliteis?

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InvisibleRoadkillM
Retired Shroomery Mod
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Registered: 12/11/01
Posts: 22,674
Loc: Montana
Trusted Cultivator
OG Cultivator
Re: B+ Growth [Re: zer0vega]
    #1235062 - 01/20/03 07:24 PM (22 years, 2 months ago)

loosen the jar lid and pull the tape off the inoculation holes.
then watch them for a few days and see if they pick back up.

if not you might have a bacterial contam.


--------------------
Laterz, Road

Who the hell you callin crazy?
You wouldn't know what crazy was if Charles Manson was eating froot loops on your front porch!

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Offlinezer0vega
stealing happyhours

Registered: 01/02/03
Posts: 307
Loc: In a van down by the rive...
Last seen: 11 years, 2 months
Re: B+ Growth [Re: Roadkill]
    #1235072 - 01/20/03 07:29 PM (22 years, 2 months ago)

they've got foil lids, wich are fairly loose

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InvisibleMagash
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Registered: 07/25/02
Posts: 5,876
Loc: Near Hilo
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Re: B+ Growth [Re: zer0vega]
    #1235176 - 01/20/03 09:26 PM (22 years, 2 months ago)

They may have dried a little.


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Offlinezer0vega
stealing happyhours

Registered: 01/02/03
Posts: 307
Loc: In a van down by the rive...
Last seen: 11 years, 2 months
Re: B+ Growth [Re: Magash]
    #1240446 - 01/22/03 03:23 PM (22 years, 2 months ago)

bump, well they're still growing, but very slowly... think it's just the strain? what things, other than temperature, can i do to help them?

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InvisibleSixCee
keep rolling
Male

Registered: 06/12/02
Posts: 3,720
Loc: US, Chicago
Re: B+ Growth [Re: zer0vega]
    #1240448 - 01/22/03 03:24 PM (22 years, 2 months ago)

Flip them upside down.


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Invisiblez@z.com
Libertarian
Registered: 10/13/02
Posts: 2,876
Loc: ATL
Re: B+ Growth [Re: zer0vega]
    #1240454 - 01/22/03 03:27 PM (22 years, 2 months ago)

I have found that B+ colonizes PF jars very slowly, but colonizes birdseed very quickly.


--------------------
"Of all tyrannies, a tyranny exercised for the good of its victims may be the most oppressive. It may be better to live under robber barons than under omnipotent moral busybodies. The robber baron's cruelty may sometimes sleep, his cupidity may at some point be satiated; but those who torment us for our own good will torment us without end, for they do so with the approval of their own conscience." - C.S. Lewis

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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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