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Professor Frink
Mycomaniac


Registered: 11/02/08
Posts: 144
Loc: USA
Last seen: 6 years, 2 months
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Alternative agar formula for cross species hybridization
#12338003 - 04/05/10 07:56 PM (14 years, 1 month ago) |
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I have a bunch of gourmet cultures now and just ordered 500 dishes. I'd like to try and cross a yellow oyster (Pleurotus cornucopiae and Pleurotus citrinopileatus) with a blue oyster (Pleurotus columbinus). However, it doesn't look like i'll be getting a hold of any western diamondback rattlesnake venom anytime soon. I thought about using different concentrations and possibly combinations of formic acid, polyethylene glycol, and niacin to attempt to perforate the mycelial cell walls as part of an alternative agar formula to induce hybridization of different species.
I am unsure of the concentrations i should start adding these ingredients to the agar and was curious if anyone else was currently experimenting with alternative agars as well. Would it be a necessity to use an LC for inoculating plates to ensure a higher success rate? I have a 2X2 laminar flow hood so it's pretty convenient for me to mass plate cultures. I intend to post my results whether success or failure but right now I'm thinking of how I'll go through the procedure.
Should i test initial growth at different formulas, then test 10 dishes of each of these "grow" capable formulas innoc'd with the yellow and blue oyster mycelium and wait for lucky crosses? Maybe based on what i'm seeing i can pick a favored formula or hypothesis and do a 100 plate test to look for a 3rd sector formation?
I think i'll just use the R7 AGAR RECIPE
2.1 L Distilled Water
50 g Light Malt Extract
34 g Agar 10 g Humus
5 g Activated carbon
1 g MgSO4
10 ml 1% KOH solution
Would the addition of 5 grams or so of yeast extract help this make this formula better for the oyster mycelium or is it unnecessary?
Thanks!
-------------------- Culture trade list Wanted Culture list: Panellus stipticus Yellow Morel
Edited by Professor Frink (04/05/10 08:17 PM)
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Professor Frink
Mycomaniac


Registered: 11/02/08
Posts: 144
Loc: USA
Last seen: 6 years, 2 months
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Re: Alternative agar formula for cross species hybridization [Re: Professor Frink]
#12346137 - 04/06/10 11:50 PM (14 years, 1 month ago) |
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bump
-------------------- Culture trade list Wanted Culture list: Panellus stipticus Yellow Morel
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iluvfungi


Registered: 06/17/09
Posts: 1,488
Loc: Oakland, CA USA
Last seen: 13 years, 6 days
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Re: Alternative agar formula for cross species hybridization [Re: Professor Frink]
#12346230 - 04/07/10 12:04 AM (14 years, 1 month ago) |
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5 minutes on google yields 10 times more useful information on advanced fungi research then this site ever would.
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Ritual
Spore Collecter

Registered: 05/05/09
Posts: 249
Last seen: 8 years, 10 months
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Re: Alternative agar formula for cross species hybridization [Re: iluvfungi]
#12346931 - 04/07/10 04:42 AM (14 years, 1 month ago) |
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Use TCBS agar with a vibrio furnissii culture. This is a bacteria from water that will have the same affect as snake venom. You might know vibrio as cholera.
The role that Vibrio furnissii plays is that it eats chitin (the cell walls of the fungi) among other things.
TCBS agar will also grow fungi.. not ideal but it works. Will work for the purpose.
Using this cholera bacteria you should be able to cross different mushroom species and bypass the snake venom requirement.
Edited by Ritual (04/07/10 09:23 AM)
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