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Offlineniall_b73
a quick learner
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Registered: 10/01/08
Posts: 508
Loc: Eire Flag
Last seen: 3 days, 9 hours
Re: liquid culture question ?? [Re: Spongiform]
    #10128540 - 04/08/09 08:24 AM (14 years, 11 months ago)

shit...... i forgot to show you guys the finished product





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next time i order a pizza i'm gonna ask them to cut it into 6 slices instead of 8, because i was'nt fit for 8 the last time !!


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Invisible200proofhonky
bluethumb
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Registered: 06/26/08
Posts: 806
Loc: Timbucktoo
Re: liquid culture question ?? [Re: Spongiform]
    #10128577 - 04/08/09 08:35 AM (14 years, 11 months ago)

Quote:

Spongiform said:
Without an air supply of some sort the mycelium will grow some, then stall out due to lack of oxygen.

So you've got two options.  One is to provide for filtered gas exchange, the other is to inject flame sterilized air.

I do all my lids (grain jars, lc's, etc) the same basic way:



Injection port + tyvek glued down over an air hole.

Little bit extra work, but they're re-usable and no fuss.

You can suck in air through a flame (slowly) into a clean syringe, then inject that into the LC jar and shake/swirl well.

It seemed like I needed to inject 20-30cc of air into a pint sized jar to get any significant growth and it would only last 2-3 days then stop growing again.

I did some comparisons awhile back and came to the conclusion that "sugar in the raw" was the cheapest, easiest sugar that produced good results to use in an LC. 

So I'd recommend that over honey, although I'm sure some people would disagree.  The sugar in the raw produces little to no discoloration or sediment and the mycelium grows well in it :-D

Plus it's already premeasured for you, 1 packet of sugar per 1 cup of water.






i use spongifoms ports too.

i use a polyfil breather hole on one side and a port on the other with tyvek under the lid.

there is no chance of the polyfil getting wet because the tyvek is there and when you tilt to suck some out you use the port.

basically the same thing he does but with polyfil instead.


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They try to take away everything God gave us,yet...
our money still says "In God We Trust".
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You take a mortal man and put him in control,
Watch him become a God,watch peoples heads a'roll.
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If you kill a man then your a murderer,kill many an your a conqueror, kill them all and your a God.

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Offlineniall_b73
a quick learner
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Registered: 10/01/08
Posts: 508
Loc: Eire Flag
Last seen: 3 days, 9 hours
Re: liquid culture question ?? [Re: 200proofhonky]
    #10128594 - 04/08/09 08:40 AM (14 years, 11 months ago)

good idea....


--------------------
next time i order a pizza i'm gonna ask them to cut it into 6 slices instead of 8, because i was'nt fit for 8 the last time !!


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Invisible200proofhonky
bluethumb
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Registered: 06/26/08
Posts: 806
Loc: Timbucktoo
Re: liquid culture question ?? [Re: kiyote]
    #10128657 - 04/08/09 08:58 AM (14 years, 11 months ago)

i would at least stuff that hole with some polyfil.

you should be able to find tyvek or something suitable.

also,you cant punch 2 holes,one on each side and cover one with rtv sealant or silicone and let it dry....stuff the other with polyfil.

you can then use the rtv or silicone sealed hole as a self healing injection port.
you also may want to PM spongiform...he used to have ports to trade.


--------------------
------------------------------------------------------------------------------------------------------------------------------
They try to take away everything God gave us,yet...
our money still says "In God We Trust".
------------------------------------------------------------------------------------------------------------------------------
You take a mortal man and put him in control,
Watch him become a God,watch peoples heads a'roll.
------------------------------------------------------------------------------------------------------------------------------
If you kill a man then your a murderer,kill many an your a conqueror, kill them all and your a God.

Extras: Filter Print Post Top
OfflineImpatient
Beginner
Female

Registered: 02/27/10
Posts: 39
Last seen: 13 years, 3 months
Re: liquid culture question ?? [Re: niall_b73]
    #12313596 - 04/01/10 06:10 PM (13 years, 11 months ago)

i found that the foil should be off,mine double there innoc time when i found that it should.this is my first hand at all this,so i did 8 jars and birthed 5(because my foil was suffocating them)no contams YET!plus just did my first lc and 5 days going GREAT so thats my opinion best of luck

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