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Shop: Myyco.com Isolated Cubensis Liquid Culture For Sale   North Spore Injection Grain Bag

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Invisiblevegas69
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Registered: 02/28/10
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Test tube
    #12313138 - 04/01/10 04:49 PM (14 years, 1 month ago)

25x 150mm with stoppers... Can they be used for masters?

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OfflinePrimalSoup
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Re: Test tube [Re: vegas69]
    #12313186 - 04/01/10 04:58 PM (14 years, 1 month ago)

I use 20x100s, so, sure!  Need screw on caps, though, for sterilizing.

-PS


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Invisiblevegas69
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Re: Test tube [Re: PrimalSoup]
    #12313200 - 04/01/10 05:02 PM (14 years, 1 month ago)

Bummer these came with stoppers

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OfflinePrimalSoup
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Re: Test tube [Re: vegas69]
    #12313688 - 04/01/10 06:25 PM (14 years, 1 month ago)

Are they autoclavable?  That's all that matters, if you're careful in storage...

PS


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if you stand too close to the machine it'll start to eat you
Primal's simple tested teks and projects: :awesomenod: Wheat Prep 2.0  Acidic Tea Tek  Potency Project! 

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Invisiblevegas69
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Re: Test tube [Re: PrimalSoup]
    #12313759 - 04/01/10 06:36 PM (14 years, 1 month ago)

this is what know... i assume they are?25 x 150mm test tubes, capacity is 55ml
Heat resistant Borosilicate glass
Rims are fire-polished for extra strengh
Fitted with #4 rubber stoppers
Are they?

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Offlinewhitelight7
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Re: Test tube [Re: PrimalSoup]
    #12313907 - 04/01/10 07:00 PM (14 years, 1 month ago)

Quote:

PrimalSoup said:
I use 20x100s, so, sure!  Need screw on caps, though, for sterilizing.

-PS




Yeah, screw caps are the best for masters, but I've gotten away with regular caps plus perifilm for about a yr. with some of my lesser fav cultures.


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Invisiblevegas69
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Re: Test tube [Re: vegas69]
    #12313927 - 04/01/10 07:03 PM (14 years, 1 month ago)

Borosilicate lab glassware can be sterilized or autoclaved by dry heat or in a steam autoclave.
Proper protocol for steam autoclaving of borosilicate glassware is 15-20 minutes at 100-120° C or 212-248f. Always leave closures off or loose during autoclaving.
The real pressure ratings depend on the wall thickness of the piece and the temperature as well. Most standard lab glass products, at room temperature, can take pressure up to 25-30 PSI. Also, be sure there are no scratches in the glass, as they will weaken the piece, which could cause it to break under pressure. Some glass vessels have very little or NO pressure rating. Most flasks or beakers are 5-15 psi maximum

So what about the stoppers?

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Invisiblevegas69
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Re: Test tube [Re: whitelight7]
    #12313947 - 04/01/10 07:06 PM (14 years, 1 month ago)

Quote:

whitelight7 said:
Quote:

PrimalSoup said:
I use 20x100s, so, sure!  Need screw on caps, though, for sterilizing.

-PS




I've gotten away with regular caps plus perifilm for about a yr. with some of my lesser fav cultures.



Well i already bought these, so if i can use them i would like to.
I am a beginner so would I not put the stopper on all the way and then wrap with a gas permeable film of some kind, like cling wrap or parafilm?

Edited by vegas69 (04/01/10 07:08 PM)

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Offlinewhitelight7
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Re: Test tube [Re: vegas69]
    #12314012 - 04/01/10 07:16 PM (14 years, 1 month ago)

Your main concern is gona be drying out over time.


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Invisiblevegas69
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Re: Test tube [Re: whitelight7]
    #12314059 - 04/01/10 07:23 PM (14 years, 1 month ago)

can i sterilize with stoppers? do i use foil instead at that point then stoppers or stoppers at time of sterilization????

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Offlinewhitelight7
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Re: Test tube [Re: vegas69]
    #12314116 - 04/01/10 07:34 PM (14 years, 1 month ago)

what are the stoppers made of?  My guess is that they can withstand that ind of heat if they came w/ the tubes


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Invisiblevegas69
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Re: Test tube [Re: whitelight7]
    #12314234 - 04/01/10 07:52 PM (14 years, 1 month ago)

ya i would agree they are rubber... but will they seep oils into tube is what i am wondering now???
I think i am going to return them.
Sucks i didn't even think of it until i opened the shipment today. the place i bout my 3 section petris has these;Economical, high-quality, borosilicate, reusable culture tubes that meet ASTM standards. All include black phenolic screw caps and are autoclaveable.
Standard Grade Glass Culture Tubes with Caps, 25 x 100 mm, Pack of 12  under 12 bucks

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OfflinePrimalSoup
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Re: Test tube [Re: vegas69]
    #12314476 - 04/01/10 08:29 PM (14 years, 1 month ago)

If they seep anything it's not good.  The alternative you mentioned is a good price, I'd go with those.

-PS


--------------------

if you stand too close to the machine it'll start to eat you
Primal's simple tested teks and projects: :awesomenod: Wheat Prep 2.0  Acidic Tea Tek  Potency Project! 

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Invisiblevegas69
humble

Registered: 02/28/10
Posts: 317
Re: Test tube [Re: PrimalSoup]
    #12314725 - 04/01/10 09:14 PM (14 years, 1 month ago)

thanks for the help

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OfflineRogerRabbitM
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Re: Test tube [Re: vegas69]
    #12319343 - 04/02/10 05:38 PM (14 years, 1 month ago)

Throw away the stoppers and just cover the tubes with foil.  After you inoculate them, punch a hole in the foil on the top and then wrap over it with parafilm.  Make sure the parafilm covers the hole in the top of the foil and seals the edges against the glass.
RR


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