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Offlinebhaas
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Bad spore syringe(s) ???
    #12234790 - 03/19/10 11:53 PM (13 years, 10 months ago)

OK, after my last debacle. Which can be seen here http://www.shroomery.org/forums/showflat.php/Number/12012479#12012479

Which by the way produced nothing whatsoever after a month. No mold, no growth of any kind and jars still smelled nice and fresh. Thru 'em away anyway and bought new ones and a pressure cooker.

Doing the WBS tek. This one here --> http://www.shroomery.org/forums/showflat.php/Number/5265629#5265629

I PC'd six 1/2 pint KERR jars and one 1 1/2 quart pickle jar. Noc'd them up last Friday and 2 of the 1/2 pint jars have a couple of little white spots which may be "MYC" who knows.

So, while I was waiting I made an LC jar with real maple syrup. I eyeballed a little bit of syrup into a 1 1/2 qt jar, added some boiled bottled water then PC'd for 20 minutes, let cool and noc'd up yesterday. I did this to see/test the syringes to see if they're any good.

I just made another LC jar (cause I was bored and have no life) I used maple syrup and just a tad of white sugar just for fun then PC'd that for an hour. Will noc it up tomorrow.

I'm trying to grow the PF Classic. Do these grow slow? Just wondering cause I thought the WBS worked fast and you'd see something decent going on in at least a week.


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InvisibleShea25
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Re: Bad spore syringe(s) ??? [Re: bhaas]
    #12234802 - 03/19/10 11:55 PM (13 years, 10 months ago)

Quote:

bhaas said:
OK, after my last debacle. Which can be seen here http://www.shroomery.org/forums/showflat.php/Number/12012479#12012479

Which by the way produced nothing whatsoever after a month. No mold, no growth of any kind and jars still smelled nice and fresh. Thru 'em away anyway and bought new ones and a pressure cooker.

Doing the WBS tek. This one here --> http://www.shroomery.org/forums/showflat.php/Number/5265629#5265629

I PC'd six 1/2 pint KERR jars and one 1 1/2 quart pickle jar. Noc'd them up last Friday and 2 of the 1/2 pint jars have a couple of little white spots which may be "MYC" who knows.

So, while I was waiting I made an LC jar with real maple syrup. I eyeballed a little bit of syrup into a 1 1/2 qt jar, added some boiled bottled water then PC'd for 20 minutes, let cool and noc'd up yesterday. I did this to see/test the syringes to see if they're any good.

I just made another LC jar (cause I was bored and have no life) I used maple syrup and just a tad of white sugar just for fun then PC'd that for an hour. Will noc it up tomorrow.

I'm trying to grow the PF Classic. Do these grow slow? Just wondering cause I thought the WBS worked fast and you'd see something decent going on in at least a week.





Making a LC is a horrible way to see if a syringe is good, Testing it on a jar is the best way. Nay cube strain can grow slow or fast its up to genetics


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OfflineDryGrain
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Re: Bad spore syringe(s) ??? [Re: Shea25]
    #12234833 - 03/20/10 12:00 AM (13 years, 10 months ago)

I would dump out the one with table sugar (sucrose) in it and make it again with a very small amount of maple syrup, since you haven't inoculated it yet.

I have an LC sitting next to my TV, it's as much of a family pet as a fish in a fishtank :smile:


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:atom:


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InvisibleShea25
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Re: Bad spore syringe(s) ??? [Re: bhaas]
    #12234846 - 03/20/10 12:03 AM (13 years, 10 months ago)

Sugar based LC's:thumbdown:

Grain Based LC's :thumbup:


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Offlinebhaas
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Re: Bad spore syringe(s) ??? [Re: Shea25]
    #12234851 - 03/20/10 12:05 AM (13 years, 10 months ago)

Well, I haven't got much to show out of 19 jars so far and my syringes
are about done. Got half of one left and I followed that WBS to the
letter to.

I could just be impatient to. Maybe I should have waited 3 weeks before
whining. Thanks.

I've read that sugar isn't the best thing to use and I'm just goofing
off. I'm gonna have that jar sitting next to me so I can keep and eye
on it. It better not act up either.


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Offlinebhaas
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Re: Bad spore syringe(s) ??? [Re: bhaas]
    #12296800 - 03/30/10 01:18 AM (13 years, 9 months ago)

Here's a couple pics of my MS LC jar after 9 days. Looks pretty tasty.

I used maple syrup and a dash of processed sugar. The shrooms from this
should taste SWEEEEEEEEET!!!!!:headbanger:





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InvisibleDoc_T
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Re: Bad spore syringe(s) ??? [Re: bhaas]
    #12297292 - 03/30/10 04:43 AM (13 years, 9 months ago)

Processed sugar isn't a very good choice for LC. :shrug:


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Offlinehamloaf
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Re: Bad spore syringe(s) ??? [Re: Shea25]
    #12297393 - 03/30/10 05:43 AM (13 years, 9 months ago)

Quote:

Shea25 said:
Sugar based LC's:thumbdown:

Grain Based LC's :thumbup:



:imwithstupid:
When making lc's less sugar is better. You do not want a hyper active mycelium that goes into shock once put to grain which has less sugar.
You want the opposite. A healthy low sugar mycelium that goes hyper active once it hits the grain that has more sugar.
It is best as with any lc to use a live culture from a petri dish.
https://files.shroomery.org/files/08-003/052619518-IMG_1423.jpg


--------------------

   
How I Get Stuff done. - My Reference Guide. - My Grows.


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Offlinebhaas
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Re: Bad spore syringe(s) ??? [Re: hamloaf]
    #12297568 - 03/30/10 06:55 AM (13 years, 9 months ago)

Quote:

hamloaf said:
Quote:

Shea25 said:
Sugar based LC's:thumbdown:

Grain Based LC's :thumbup:



:imwithstupid:
When making lc's less sugar is better. You do not want a hyper active mycelium that goes into shock once put to grain which has less sugar.
You want the opposite. A healthy low sugar mycelium that goes hyper active once it hits the grain that has more sugar.
It is best as with any lc to use a live culture from a petri dish.
https://files.shroomery.org/files/08-003/052619518-IMG_1423.jpg




I was trying to get something going cause my MS WBS jars are goin real slow. Been 3 weeks and barely got anything goin so I made an LC cause I was runnin out of MS syringes.

Must have got a slow growing strain of PF Classic.


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OfflineSpongiform
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Re: Bad spore syringe(s) ??? [Re: bhaas]
    #12297673 - 03/30/10 07:44 AM (13 years, 9 months ago)

I did some testing with different household sugars awhile back, the results might help you.

The only processed sugar from the supermarket that's worth a shit is sugar in the raw.


--------------------
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Spongi's PF Block Tek & Automated Shotgun Project.


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Offlinebhaas
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Re: Bad spore syringe(s) ??? [Re: Spongiform]
    #12300647 - 03/30/10 04:53 PM (13 years, 9 months ago)

Quote:

Spongiform said:
I did some testing with different household sugars awhile back, the results might help you.

The only processed sugar from the supermarket that's worth a shit is sugar in the raw.




I've seen that before. Great test. I was surprised raw sugar did so well. I was just messing around with this jar. It's going real good. I have no complaints so far. It's not just straight table sugar, it's maple syrup and just a tad of table sugar.

Seems to be doing better then my other jar of just maple syrup.

I also swirl it around real good everyday.


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InvisibleDoc_T
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Re: Bad spore syringe(s) ??? [Re: hamloaf]
    #12301202 - 03/30/10 06:32 PM (13 years, 9 months ago)

Quote:

hamloaf said:
You do not want a hyper active mycelium that goes into shock once put to grain which has less sugar.
You want the opposite. A healthy low sugar mycelium that goes hyper active once it hits the grain that has more sugar.





What? No, that's nonsense.
You need a low sugar concentration so the solution isn't hyperosmotic, inhibiting cell growth.
4% by weight of simple sugars like dextroseor fructose are fine.

Sucrose just isn't usable at any concentration.


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Offlinehamloaf
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Re: Bad spore syringe(s) ??? [Re: Doc_T]
    #12301704 - 03/30/10 08:06 PM (13 years, 9 months ago)

Quote:

Doc_T said:
Quote:

hamloaf said:
You do not want a hyper active mycelium that goes into shock once put to grain which has less sugar.
You want the opposite. A healthy low sugar mycelium that goes hyper active once it hits the grain that has more sugar.





What? No, that's nonsense.
You need a low sugar concentration so the solution isn't hyperosmotic, inhibiting cell growth.
4% by weight of simple sugars like dextroseor fructose are fine.

Sucrose just isn't usable at any concentration.



Think so??
LME has traces of DEX.
Might not seem like much but it is enough.


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How I Get Stuff done. - My Reference Guide. - My Grows.


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InvisibleDoc_T
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Re: Bad spore syringe(s) ??? [Re: hamloaf]
    #12301736 - 03/30/10 08:10 PM (13 years, 9 months ago)

Very, very few organisms can eat refined white sugar, and really nothing can live on that alone.

Edit going ninja, eh?

Neither malt nor DEX are sucrose. Malt+dex makes a nice LC, up to about 4%.

So what's your point?


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You make it all possible. Doesn't it feel good?


Edited by Doc_T (03/30/10 08:18 PM)


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Offlinehamloaf
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Re: Bad spore syringe(s) ??? [Re: Doc_T]
    #12301849 - 03/30/10 08:36 PM (13 years, 9 months ago)

Well Doc my point is that I use 1 gram of LME per 500ml of water for my LC's And have had much higher rate of succession rate plus faster growth then when I have used LME/DEX at 1 teaspoon per 500ml of water and when it hits the grain it's off.
I ran them side by side the last time I did LC's.
Forget about it I will never use any kinda sugar, honey, karo, or DEX again.
lol.

:2cents: :starwars:


--------------------

   
How I Get Stuff done. - My Reference Guide. - My Grows.


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InvisibleDoc_T
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Re: Bad spore syringe(s) ??? [Re: hamloaf]
    #12302738 - 03/30/10 11:02 PM (13 years, 9 months ago)

Well, you could get butthurt or you could go back and read what I said, which is:
"don't use sucrose, and don't exceed 4% by weight". Ok with me either way, I know how to make LC.


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You make it all possible. Doesn't it feel good?


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InvisibleTacoHerder
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Re: Bad spore syringe(s) ??? [Re: Doc_T]
    #12302740 - 03/30/10 11:03 PM (13 years, 9 months ago)

to each his own.


--------------------
TO ALL CULTIVATORS, EVERYTHING YOU NEED TO KNOW http://www.shroomery.org/forums/showflat.php/Number/10899385
We need more member like mycochef, he is truly a kind giving person. Just ask Noobie, mycochef hooked him up big time!
Fastest Cultivation WINNER!!!! NoOneKnowsHowToAct won it twice, must be doing something right:super:

Shoot me a pm if you would like a powerful custom laser built. :smile: 4th pic is a 1.3W 445nm laser burning thru some plastic makeup containers. Yes I was wearing proper safety goggles, otherwise I would be blind right now.


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InvisibleDoc_T
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Re: Bad spore syringe(s) ??? [Re: TacoHerder]
    #12302746 - 03/30/10 11:05 PM (13 years, 9 months ago)

You're saying you use sucrose for liquid culture?


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You make it all possible. Doesn't it feel good?


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Offlinehamloaf
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Re: Bad spore syringe(s) ??? [Re: Doc_T]
    #12302762 - 03/30/10 11:08 PM (13 years, 9 months ago)

Quote:

Doc_T said:
Well, you could get butthurt or you could go back and read what I said, which is:
"don't use sucrose, and don't exceed 4% by weight". Ok with me either way, I know how to make LC.



You are completely insane sometimes DOc.
I know how to make a few LC's also.
If you would go back and read what I said.
I never mentioned sucrose anything.
My anal is not aching.
Sounds like you have projection issues. lol


--------------------

   
How I Get Stuff done. - My Reference Guide. - My Grows.


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InvisibleTacoHerder
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Re: Bad spore syringe(s) ??? [Re: Doc_T]
    #12302778 - 03/30/10 11:11 PM (13 years, 9 months ago)

Quote:

Doc_T said:
You're saying you use sucrose for liquid culture?





dont we all :awedrugs:


--------------------
TO ALL CULTIVATORS, EVERYTHING YOU NEED TO KNOW http://www.shroomery.org/forums/showflat.php/Number/10899385
We need more member like mycochef, he is truly a kind giving person. Just ask Noobie, mycochef hooked him up big time!
Fastest Cultivation WINNER!!!! NoOneKnowsHowToAct won it twice, must be doing something right:super:

Shoot me a pm if you would like a powerful custom laser built. :smile: 4th pic is a 1.3W 445nm laser burning thru some plastic makeup containers. Yes I was wearing proper safety goggles, otherwise I would be blind right now.


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