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Offlineerrorserror
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Sterilization through boiling - Twice and time in between?
    #1228638 - 01/18/03 11:13 AM (14 years, 8 months ago)

Hi there,
This has probably been asked before but I have been searching and could not find a direct answer. Here is the situation: I have 12 jars which were boiled for an hour one thursday afternoon. Something came up and the jars could not be inoculated. If the jars were re-sterilized the following monday and then innoculated several hours later, would any problems arise? The jars will have been sitting, covered in tin foil in their original box during the span. Is it worth trying this or just remixing the substrate in new jars. I also have 1 jar of honey water under the same circumstances. Thanks for your help! J


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OfflineFunker
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Re: Sterilization through boiling - Twice and time in between? [Re: errorserror]
    #1228740 - 01/18/03 11:54 AM (14 years, 8 months ago)

I dont know if resterilizing them would affect it, but I once prepared and sterilized the substrates one day and didnt innoculate until about a week or two later because I had no time. One jar did get contaminated but I think you should be ok, as long as nothing dried out, which i doubt.


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OfflineISH
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Re: Sterilization through boiling - Twice and time in between? [Re: Funker]
    #1229724 - 01/18/03 07:41 PM (14 years, 8 months ago)

If it's been a week or more since sterelized, and there is no sign of infection, I wouldn't even resterelize them I would use them. By now if there were any contams evident, you would see them.

My 2 cents anyway.

ISH


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Offlineerrorserror
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Re: Sterilization through boiling - Twice and time in between? [Re: ISH]
    #1230245 - 01/19/03 03:54 AM (14 years, 8 months ago)

Yeah, I unless there are contaminants in there that will take over, i cant imagine any problem, since theres nothing getting in there; the jars are tightly wrapped in foil. I have read that not everything is killed, especially when just boiling. We'll see. Anyone else have any input? Thanks guys for the help . J


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Mushrooms, Mycology and Psychedelics >> Mushroom Cultivation

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