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Registered: 08/25/01
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Too much h2o2 agar?
    #1226786 - 01/17/03 02:10 PM (14 years, 8 days ago)

This is a pic of a piece of agar which has a piece of agar clone on it. Frank recently transfered it to agar that was preped with 3 ml of h2o2 and 300ml of MEA. The growth around the tranfered piece is wispy to a point of looking like bacterial contam. Frank is wondering if it is bacterial contam or the high h2o2 content of the MEA is to blame for this weak growth?

Thanks...... :confused:

Support bacteria - they're the only culture some people have.

Edited by upupup (03/13/03 08:11 PM)

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Re: Too much h2o2 agar? [Re: upupup]
    #1226806 - 01/17/03 02:20 PM (14 years, 8 days ago)

P. Cubensis? I usually add 3cc's of peroxide to a 500ml agar mixture, when making peroxidated agar, but i doubt your concentration would have made it unviable.Looks to me like that's just early growth, in a couple days you should know for sure.


-- The information I provide is only information from readings, growing of gourmet mushrooms, and second hand stories--

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Re: Too much h2o2 agar? [Re: upupup]
    #1227104 - 01/17/03 05:05 PM (14 years, 8 days ago)

looks more like there isnt enough agar in the mix and its not very solid. that amount of h202 is very low - i usually use around 5mls per 250mls, but that's just me :smile:


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Mushrooms, Mycology and Psychedelics >> Advanced Mycology

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