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Amazon Shop for: ½ Pint Jars, Laminar Flow Hood, Vermiculite, Wild Bird Seed

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OfflineAnnoA
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The Importance of Gas Exchange
    #1226637 - 01/17/03 01:18 PM (13 years, 10 months ago)

When mushrooms digest a substrate, they produce carbon dioxide, and consume oxygen.
This is a fairly important factor determining success or demise of a growth attempt.

I prepared a few plastic containers with 1/2 pint content with my standard substrate I use for PFtek jars.The plastic containers have a lid, that completely seals the container, so there is no gas exchange.

When I use the same substrate in jars with an aluminum foil lid, like described at www.fungifun.org I get full colonization in 8-12 days.

The spores germinated after 2 days and the colonization went fairly fast for another 3 days, then it suddenly stopped.

I left is then another week, and the colonization barely moved on.
Then I opened the container in font of a flow hood for a few seconds to provide some air.
The colonization again started, and again halted after a few days....
I repeated this another 2 times, and after 1 month into colonization, the substrate is still not completely colonized.
You can see at the pictures how the mycelium has a different appearance in some areas and it shows the different growth and stall phases.

The inoculation was at the sides and one inoculation point at the top of substrate. There is no vermiculite seal, but a thin vermiculite bottom layer.





So, people, never seal your jars.....



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Offlineajg2425
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Re: The Importance of Gas Exchange [Re: Anno]
    #1226713 - 01/17/03 01:43 PM (13 years, 10 months ago)

thats not completley accurate...you should try doing it with like 8sealed and 8unsealed to get better information.


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InvisibleSixCee
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Re: The Importance of Gas Exchange [Re: Anno]
    #1226724 - 01/17/03 01:46 PM (13 years, 10 months ago)

Yeah I never seal them. After sterilization I rip that tin foil off and leave it like that.


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OfflineGratefulDread
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Re: The Importance of Gas Exchange [Re: Anno]
    #1226735 - 01/17/03 01:50 PM (13 years, 10 months ago)

Yet another useful informative post anno thanks.

For quite a while now I?ve been using drinking glasses instead of wide mouth jars becuse they are cheaper, easier to get the colonized cake out of and seem to colonize faster.

One thing I have noticed though is that the foil lids seem to allow some moisture escape and I was wondering if maybe the mycellium is colonizing faster because the substrate is drier?

Here?s a couple of bad webcam pics of some glasses prepared with a foil lid and fully colonized. I know you can?t see them well but it does give you a basic idea





I cross the tape over the top before innoculating to keep the foil from ripping at the innoculation points and then retape it after to keep out contams



--------------------
Before you criticize someone, you should walk a mile in their shoes.
That way, when you criticize them, you're a mile away and you have their shoes.


Edited by GratefulDread (01/17/03 01:52 PM)


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OfflineGratefulDread
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Re: The Importance of Gas Exchange [Re: Anno]
    #1226736 - 01/17/03 01:50 PM (13 years, 10 months ago)

Yet another useful informative post anno thanks.

For quite a while now I've been using drinking glasses instead of wide mouth jars becuse they are cheaper, easier to get the colonized cake out of and seem to colonize faster.

One thing I have noticed though is that the foil lids seem to allow some moisture escape and I was wondering if maybe the mycellium is colonizing faster because the substrate is drier?

Here's a couple of bad webcam pics of some glasses prepared with a foil lid and fully colonized. I know you can't see them well but it does give you a basic idea

[image] [/image]

[image]
[/image]

I cross the tape over the top before innoculating to keep the foil from ripping at the innoculation points and then retape it after to keep out contams



--------------------
Before you criticize someone, you should walk a mile in their shoes.
That way, when you criticize them, you're a mile away and you have their shoes.


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InvisibleEthnoJimmy
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Re: The Importance of Gas Exchange [Re: SixCee]
    #1226738 - 01/17/03 01:50 PM (13 years, 10 months ago)

I leave the foil on, but unscrew the lids just a touch.


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InvisibleSixCee
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Re: The Importance of Gas Exchange [Re: EthnoJimmy]
    #1226742 - 01/17/03 01:51 PM (13 years, 10 months ago)

I dont unscrew them at all and leave them on tight. I rely on the verm. barrier to keep out contams but this micorice tek I am doing now I might have to shake my ajrs which may mess everything up.


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InvisibleEthnoJimmy
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Re: The Importance of Gas Exchange [Re: SixCee]
    #1226748 - 01/17/03 01:53 PM (13 years, 10 months ago)

I use wbs, and don't use a verm seal. You're right, it'll suck if you have to shake it.


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Don't walk behind me, I will not lead?
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OfflineAnnoA
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Re: The Importance of Gas Exchange [Re: ajg2425]
    #1226780 - 01/17/03 02:09 PM (13 years, 10 months ago)

>thats not completley accurate...you should try doing it with like 8sealed and
>8unsealed to get better information.

Will those be enough?



I can?t show you the regular cakes, since they were done and away a long time


Edited by Anno (01/17/03 11:48 PM)


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Offlinevatoloco
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Post deleted by MOE THE MAD SCIENTIST [Re: Anno]
    #1226799 - 01/17/03 02:16 PM (13 years, 10 months ago)



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InvisibleEthnoJimmy
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Re: The Importance of Gas Exchange [Re: vatoloco]
    #1226941 - 01/17/03 03:18 PM (13 years, 10 months ago)

I use foil over the regular lid. I just lift the foil a touch and inoc through a hole in the top of the lid.


--------------------
Don't walk behind me, I will not lead?
Don't walk in front of me, I will not follow?
Just walk beside me and be a friend.



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InvisibleTinMan
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Re: The Importance of Gas Exchange [Re: Anno]
    #1227032 - 01/17/03 04:18 PM (13 years, 10 months ago)

I noticed your containers are next to your computer. Do you use your tower to incubate them in?


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Anonymous

Re: The Importance of Gas Exchange [Re: Anno]
    #1227033 - 01/17/03 04:18 PM (13 years, 10 months ago)

:blush: 


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OfflineAnnoA
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Re: The Importance of Gas Exchange [Re: TinMan]
    #1227039 - 01/17/03 04:21 PM (13 years, 10 months ago)

>I noticed your containers are next to your computer. Do you use your tower to incubate them in?

No, this is just an old pentium laying around and waiting that I find time and will to set it up for making time laps photos...


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InvisibleSixCee
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Re: The Importance of Gas Exchange [Re: Anno]
    #1227114 - 01/17/03 05:09 PM (13 years, 10 months ago)

I have the regular lids on screwed tight. After sterilization I take off the foil and throw it out.


--------------------
~~~~~~~~~~~~~~~~~~~~~~~~
-->The above statements may or not be true.
->Quote of the Moment :
"Yea. All bitches are whores who love sex." -Cubie
----> PMs checked daily.


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OfflineLoop_Theorist
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Re: The Importance of Gas Exchange [Re: ajg2425]
    #1227137 - 01/17/03 05:18 PM (13 years, 10 months ago)

Tell me you did NOT just question Anno's methods...

-Loop


--------------------
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Some take tha plane, you, you on tha boat,
right beside tha money which right beside tha coke
Passin by tha coastgaurd, right under their nose.

To the US-HEY where anyone can getny, MIA all the way to New Your city, you a twenty now....


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InvisibleSixCee
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Re: The Importance of Gas Exchange [Re: Loop_Theorist]
    #1227145 - 01/17/03 05:20 PM (13 years, 10 months ago)

Who me!? No not me! I swear you got the wrong guy! No its not me Anno please..no, no.......NNNNOOOOOOOOO, aaaaaaaaggghhhhhhhh!!!!!!!


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~~~~~~~~~~~~~~~~~~~~~~~~
-->The above statements may or not be true.
->Quote of the Moment :
"Yea. All bitches are whores who love sex." -Cubie
----> PMs checked daily.


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OfflineAnnoA
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Re: The Importance of Gas Exchange [Re: SixCee]
    #1227762 - 01/17/03 10:11 PM (13 years, 10 months ago)

No, not you the reply wa to ajg2425.

Good luck with the sealed jars.


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Offlinehappygrins
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Re: The Importance of Gas Exchange [Re: Anno]
    #1227805 - 01/17/03 10:38 PM (13 years, 10 months ago)

Does this same rule apply when your spawning your Poo/Straw?  Does having holes in your aluminum foil opposed to not having holes in the aluminum foil change colonization times?

--happygrins :grin: 


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OfflineAnnoA
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Re: The Importance of Gas Exchange [Re: happygrins]
    #1227866 - 01/17/03 11:47 PM (13 years, 10 months ago)

No. Aluminium foil put over anything provides enough gas exchange. I didn?t notice a difference between with or without holes.


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Amazon Shop for: ½ Pint Jars, Laminar Flow Hood, Vermiculite, Wild Bird Seed

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