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kaioh813


Registered: 02/26/10
Posts: 130
Loc: Under the Firmament
Last seen: 5 months, 10 days
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Please help unknown yellow contam
#12251335 - 03/22/10 08:33 PM (14 years, 9 months ago) |
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Does anyone know what this is, myc piss or a contam? Appeared 3 days after innoc. I heard sometimes you can knock some contams out, is that true?
http://img51.imageshack.us/g/picture003e.jpg/
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Masticore
Perpetually Curious



Registered: 12/16/09
Posts: 1,159
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Re: Please help unknown yellow contam [Re: kaioh813]
#12251457 - 03/22/10 08:50 PM (14 years, 9 months ago) |
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I can't imagine you beating contaminates while the jar stays sealed to let the mycelium colonize. Looks like the color of "myc piss", but I've only seen those liquids gather on the bottom of a jar.
-------------------- Treat my posts like the Bible. I am fallible, subject to human limitation, and usually open to interpretation.
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Captain Caveman
Uh-oh!



Registered: 01/12/09
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Re: Please help unknown yellow contam [Re: Masticore]
#12252778 - 03/23/10 01:03 AM (14 years, 9 months ago) |
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Toss the shit. Dont even open it.
-------------------- "Captain CAAAAAAAVEMAAAAAAAAANNNN!!!!"
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BelovedLaughter
BelovedLaughter



Registered: 02/23/10
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Re: Please help unknown yellow contam [Re: kaioh813]
#12252816 - 03/23/10 01:13 AM (14 years, 9 months ago) |
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It's not mycelium metobolites. It's contamination. You could wait and see, but the odds of the mycelium outrunning it under these circumstances are slim to nil.
-------------------- Rev. Beloved Laughter
Church of the Aztec Sun
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scatmanrav
Brainy Smurf


Registered: 05/08/04
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Id say about 95% sure thats contamed..
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kaioh813


Registered: 02/26/10
Posts: 130
Loc: Under the Firmament
Last seen: 5 months, 10 days
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Re: Please help unknown yellow contam [Re: scatmanrav]
#12253832 - 03/23/10 07:53 AM (14 years, 9 months ago) |
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Well thanks for the help, do you have any ideas what caused this to happen in each jar? The first ones my friend did are now at 2 weeks and look pretty healthy. But these ones were used with an already open bag of brf and verm, not brand new, could that've been the problem since sterilization procedures were the same?
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scatmanrav
Brainy Smurf


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Re: Please help unknown yellow contam [Re: kaioh813]
#12254375 - 03/23/10 10:17 AM (14 years, 9 months ago) |
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No the open bag isnt gonna be a problem..either something got in or on the syringe (you sterilize the needle right?)..or something got through the verm layer..maybe it got wet? Lots of ways to get contams..
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kaioh813


Registered: 02/26/10
Posts: 130
Loc: Under the Firmament
Last seen: 5 months, 10 days
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Re: Please help unknown yellow contam [Re: scatmanrav]
#12254685 - 03/23/10 11:08 AM (14 years, 9 months ago) |
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Well thats good atleast the verm an brf is still usable...
Everything was sterilized with iso alc, the jars were washed clean, syringe had been in refrigerator in ziploc bag, sterilized needle with iso alc, flame sterilized, wiped syringe between each hole... I'm thinking it might have been from sterilizing the jars in the pot because a towel was used to elevate them out of the water, and when the jars were pulled out the water had turned a weird milky color... no clue how. Maybe from the towel.
Anyways hope the other jars are good, can contams appear at any point when mycelium is growing? Because the contams started showing up in like 2 days, along with mycelium, when it took all other jars atleast a week before any signs of mycelium.
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punkrocker292004
i am a liar


Registered: 12/17/09
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Re: Please help unknown yellow contam [Re: kaioh813]
#12254704 - 03/23/10 11:14 AM (14 years, 9 months ago) |
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''wiped syringe between each hole'' there is your problem you need to flame between each jar
-------------------- EVERYTHING YOU NEED TO KNOW
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kaioh813


Registered: 02/26/10
Posts: 130
Loc: Under the Firmament
Last seen: 5 months, 10 days
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Actually I think it was because of the water used to make the cakes, could that be the problem? I've heard some people say to use distilled, others sink, others lake. So I didn't think using a recently opened jug of water out the fridge would cause any contams. Because the next few jars that were done are now just 4 days old, same procedure and sterilized between every jar. But it looks like the same color is appearing again right at every innoc hole, a little too early to tell but I've thought of every possibility an its getin to me. Any help is much appreciated!
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rberg79
Psychonaut

Registered: 03/02/10
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Quote:
punkrocker292004 said: ''wiped syringe between each hole'' there is your problem you need to flame between each jar
I'm assuming he's wiping it with the alcohol, which would be okay.
OP, I'm thinking you didn't sterilize your substrate after all. What procedure did you use? Boil it on the stove for a while? If so, that's your problem. A pressure cooker is a worthwhile investment, I swear.
-------------------- &T
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kaioh813


Registered: 02/26/10
Posts: 130
Loc: Under the Firmament
Last seen: 5 months, 10 days
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Re: Please help unknown yellow contam [Re: rberg79]
#12306610 - 03/31/10 03:43 PM (14 years, 9 months ago) |
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yeah with alcohal, figured it would be, this time made sure to flame a lot. and yeah im just boilin it for 90 minutes.. no problems with the first ones though. Could you explain the reason for pressure cooking, i seemed to have missed it, does it sterilize the mixture of the jar? If so then the water i used couldnt have been the problem right?
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RogerRabbit
Bans for Pleasure


Registered: 03/26/03
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Re: Please help unknown yellow contam [Re: kaioh813]
#12307040 - 03/31/10 04:59 PM (14 years, 9 months ago) |
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I won't open imageshack pictures, but if it's contaminated, toss it out. Flame sterilize between jars. Also, you don't boil for 90 minutes, you steam sterilize for 90 minutes and don't start counting the time until steam escapes from under the tight fitting lid. The BRF tek part 2 video gives a demonstration of sterilizing in steam. You don't need a pressure cooker for the brf tek.
The most likely cause if all your jars contaminated the same way is a bad syringe. RR
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"I've never had a failed experiment. I've only discovered 10,000 methods which do not work."
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JonEveryman88
ಠ_ಠ



Registered: 01/18/10
Posts: 1,638
Loc: Land of Maple Syrup
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Re: Please help unknown yellow contam [Re: RogerRabbit]
#12307058 - 03/31/10 05:02 PM (14 years, 9 months ago) |
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Quote:
The most likely cause if all your jars contaminated the same way is a bad syringe.
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anonjon
Partially Right

Registered: 11/03/08
Posts: 6,322
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Re: Please help unknown yellow contam [Re: JonEveryman88]
#12307144 - 03/31/10 05:15 PM (14 years, 9 months ago) |
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looks like yellow slime. also seems to be some cobweb in there.
Edited by anonjon (03/31/10 05:18 PM)
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JonEveryman88
ಠ_ಠ



Registered: 01/18/10
Posts: 1,638
Loc: Land of Maple Syrup
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Re: Please help unknown yellow contam [Re: anonjon]
#12307161 - 03/31/10 05:17 PM (14 years, 9 months ago) |
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That's a really big photo, lol.
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kaioh813


Registered: 02/26/10
Posts: 130
Loc: Under the Firmament
Last seen: 5 months, 10 days
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Re: Please help unknown yellow contam [Re: RogerRabbit]
#12307380 - 03/31/10 05:57 PM (14 years, 9 months ago) |
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Sorry I didnt mean boil for 90 minutes, typo. I been following the vids exactly and I research the threads a lot, but I do not cover the holes with tape during steam sterilization, just foil wrap 3 pieces around and make sure there arent holes in the foil. Should I be using tape for the holes? I used a different syringe than last time, different sponsors, brand new placed right in the fridge where theyre kept and flame sterilized between all jars. Im not 100% sure the jars just recently done with the new syringe are contaminated, but there is a weird color at every innoc point like a grey stain with a yellowish tint to it again.
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JonEveryman88
ಠ_ಠ



Registered: 01/18/10
Posts: 1,638
Loc: Land of Maple Syrup
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Re: Please help unknown yellow contam [Re: kaioh813]
#12307383 - 03/31/10 05:58 PM (14 years, 9 months ago) |
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No it's best to use foil during steaming; it stops water from sloshing into jars messing up water content.
Pics?
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kaioh813


Registered: 02/26/10
Posts: 130
Loc: Under the Firmament
Last seen: 5 months, 10 days
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Re: Please help unknown yellow contam [Re: anonjon]
#12307543 - 03/31/10 06:23 PM (14 years, 9 months ago) |
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Im not sure what cobweb looks like, Could you take a look at my colonising jars and tell me if im missing the cobweb? http://img30.imageshack.us/g/sany0072l.jpg/
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JonEveryman88
ಠ_ಠ



Registered: 01/18/10
Posts: 1,638
Loc: Land of Maple Syrup
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Re: Please help unknown yellow contam [Re: kaioh813]
#12307556 - 03/31/10 06:25 PM (14 years, 9 months ago) |
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I don't mean to be a dick, but please upload images to the shroomery next time. Some people don't even open imageshack files...
Those jars look fine to me
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