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iluvfungi


Registered: 06/17/09
Posts: 1,488
Loc: Oakland, CA USA
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Re: transferring agar cultures [Re: mycoelf]
#12332771 - 04/04/10 11:24 PM (14 years, 1 month ago) |
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Unless I missed something, if all you are doing is keeping cultures, isolating cultures, growing fungi; it ain't that complicated.
Perhaps some people like Paul, who are much more intellectual then the simple hobby of growing fungi, blow it up just a little bit too much.
It only gets complicated if you are going into the genetic level of fungi, then it is very complex. But still, with time even something that is complex is no longer complex.
This just goes to show the sad state of the philosophy of science; I see something, it happens a few times, I call it factual knowledge. Given that people work as one, and everyone experiences group think, it makes one question any and all scientific knowledge.
Back on topic; transferring agar cultures, working with agar was the simplest thing I have ever learned in my entire life. It is just so natural. If you have a sterile environment, contamination is almost like a blessing to do more work, since it happens so infrequently.
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PrimalSoup
hyperspatial illuminations



Registered: 11/17/09
Posts: 13,568
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Re: transferring agar cultures [Re: iluvfungi]
#12338922 - 04/05/10 10:10 PM (14 years, 1 month ago) |
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Quote:
it ain't that complicated.

Nah, you're right. The simply doing part isn't all that complicated once you get it sussed.
But the "now why the hell did that happen?" part is something else entirely. And tends to be far more interesting as well...
BTW this is a great thread. 
Peace -PS
--------------------
if you stand too close to the machine it'll start to eat youPrimal's simple tested teks and projects: Wheat Prep 2.0 Acidic Tea Tek Potency Project!
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Mikeallojee
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Re: transferring agar cultures [Re: PrimalSoup]
#12340192 - 04/06/10 02:08 AM (14 years, 1 month ago) |
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Thank you for all the information on agar, you have saved me many years of trial and error. I read that you do some deep archival research, you must have been doing this for several years?
I will definitely do the agar to grain master. Today I PC three myco bags of wood chips I will spawn with Ps. Cyan. I read Faukface's tek on out door growing. He wrote he doesn't have problem with contamination with the woodchips and outdoor grows. That cyan mycelium must be some aggressive shit to move through and take over the way it does. Mother nature is awsome.
The problem I have is that I am in a rental and will be moving soon so I want to take my chips with me. Plus I want to be successful so I sterilized the chips. Prior I soaked in a coffee/gypsum soup with lots of water. I wasn't sure if I should add coffee or gypsum so I said what the heck in the name of science why not. I was planning on adding one quart of grain to each bag I'm not sure if that will be too much or not. I plan on placing the inoculated chips into some of my planter pots and some of my bonsai pots. I have no idea if this will work but hopefully the chips will stay conlonized so when I get someplaced more perminent I can just transplant. Not sure if I have a chance in hell but you never know unless you try or you get a better idea to try something different.
Hopefully I will get a chance to make up some agar this weekend, I want to expand the specimen on agar to begin isolation. It sounds like a kick to do it. I really need to get a flow hood so I can have some free movement. Working in a dead air box is so confining it seems like I am always fumbling around in it. Again thank you for your years of advice. myc
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mycoelf
Agent Of Chaos



Registered: 06/26/09
Posts: 555
Loc: hyperspace
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Re: transferring agar cultures [Re: Mikeallojee]
#12340956 - 04/06/10 08:42 AM (14 years, 1 month ago) |
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It is my pleasure to serve. That is part of my personal purpose being involved, to find those who want to know and open the doors that are available to me to open. My years in are but a mere Decade, But my background as a student goes back a life time. When I got interested in mushies I sought out all experience in the friend collective, many of which are Old hippy types, they were glad to offer what information they had, part of which was the original Oss and Oeric book written by the late great Terrance Makenna, and his bro under pen names. As a side note It stated that fact about P. Car, that I have not seen restated anywhere. I think that is why there is so little interest In this species, it is listed as mildly active in the lit, but the strain that I have is Var. Mazatecorum, which all of my freinds agree is the most mind blowing mushie that I have.
Good luck with your grow, I love your potted plant idea, maybe a mulch to preserve moisture would help, I think it will work. You never know you may stumble upon some unknown symbiosis.
My teacher told me that when he started his first flow hood was made from a HEPA furnace filter, that is only 1" thick and considerably cheaper. If you ae going to build a nice box plan one to accept a proper hepa, but a cheap hepa may get you by in the meantime. I think that the duct tape approach has a value to serve the short term goals and adds greatly to long term understanding. Stametes wrote that failure is a profound teacher, and I believe that to be true
Gypsum is definitely beneficial, it helps in many ways but lacks the ability to buffer PH. Coffee grounds I would guess are acidic, so watch your PH at make up. You can buffer your mix with aglime or oyster shells, the latter of which will only buffer when acids are present, the former of which should be added in at around 2-4% by mass.
Peace Mycoelf
-------------------- Mycoelf Sterility is a process that can be likened unto infinity, which is a long walk, the closer to the end you start before beginning, the more achievable the goal of infinity becomes. Remember, cleanliness in next to goddessness
      
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Mikeallojee
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Registered: 10/02/09
Posts: 897
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Re: transferring agar cultures [Re: mycoelf]
#12343741 - 04/06/10 05:25 PM (14 years, 1 month ago) |
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I appreciate your help and you taking the time to answer my noob questions. If you don't mind I would like to continue picking your mind as time goes on and I continue my work. I am unfamilure with Var. Mazatecorum. You said mind blowing Im not sure what you mean by this? I don't dabble in psych too much so they all blow my mind. I have noticed in myself that the PE gives me better visuals than the other strain I have going. The other strain is a mystery to me. I bought some mushies at a phish show a while back and broke open a closed cap and placed one of the gills on agar and it grew very well. I had no contamiation which still astonishes me. Something odd happend when I had grown it out three times, I had a white colored mushies pop up. I expected it to turn to a darker color but it stayed white. I decided to clone it to see if I can get more white ones. I have no idea at this point if there is any active properties but I am hopeful. myc
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mycoelf
Agent Of Chaos



Registered: 06/26/09
Posts: 555
Loc: hyperspace
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Re: transferring agar cultures [Re: Mikeallojee]
#12349143 - 04/07/10 02:24 PM (14 years, 1 month ago) |
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Hello,
It is my pleasure and my service to continue to correspond. Part of a Mushroom inspired mission to be a point of inoculum, also this is how I can give back to the community folks who put on the site, I could keep my viewpoint to myself, but a seed that is not planted does not grow.
By mind blowing I mean the general feed back that I get is that the strain that came to me from and old seasoned hippie "mazatec" I believe to fit the profile of P Caerulescens, physiologically, I have not had the resources to do any microscopy, some day I will and know forever for certain. There is a Var called Mazetecorum, that I feel that this strain is, This is the shroom in my signature pic. If anyone else has a more educated opinion I am dying to hear it, but by mind blowing I mean that it lends its experience towards the spiritual mystical realm, not really a party trip, but putting one in a state of in tune with the green force of nature, everything has a purpose, and is Karmicly bound to serve that purpose, any entity not serving its purpose risk extinction, The purpose of Psilocybes in my meager opinion is to serve the interface between humans and the rest of life on earth/ cosmos, why else would they produce those psi compounds that appear to have no other purpose?? That is their purpose, to speed our evolution so that we can become tech monkeys and carry life as a whole to other planets in space!!
But I digress, back on thread,
I would love it if you would share a pic or two of your fruiting cultures. I have extracted genetic material from the center of such sterile fruit and generated strains that were legendary and memorable to my cultivation experience.
Mycolight find you,
Mycoelf
-------------------- Mycoelf Sterility is a process that can be likened unto infinity, which is a long walk, the closer to the end you start before beginning, the more achievable the goal of infinity becomes. Remember, cleanliness in next to goddessness
      
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Mikeallojee
Coolaid smile



Registered: 10/02/09
Posts: 897
Loc: SW WA
Last seen: 11 years, 3 months
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Re: transferring agar cultures [Re: mycoelf]
#12351288 - 04/07/10 07:52 PM (14 years, 1 month ago) |
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http:// http:// http:// http:// http://
Here is that weird white one that popped up. I have no idea if it will continue to produce white fruit but it was cloned. I did three transfers and all look okay as of a few days ago. Once I get a grain transfer and fruit it I will know more. Hopefully I can get it to spore out white fruit after a few hundred cycles of isolation. We will see.
The majority of my other strains are not actives. I have a bunch of prints that I have traded for but as I said before I don't trip much so I use what I have and give most of them away or just throw them out with the trash. Some may feel this is a waist but for me its more about the growing than then consuming. Consuming is a nice bye product of a kick ass hobby.
I will be honest, I got into cultivation last fall because I got so frustrated not being able to find any cyan's. I live in the PNW about an hour from Astoria and couldn't find shit. It pissed me off so bad that i became motivated. I think I spent over a hundred hours shrooming last fall. I found tons of cool mushrooms and one azure.The other problem was that there were state troopers all over out at fort stevens and that really sketched me out. The kids are getting good at finding them and we work on identifying as many as we can. Its funny because we go down by the river all of us in our rain gear tromping through the trees and brush.
The other reason I got into cultivation is that I love to grow things and typically have a garden and bonsai but there isn't much to do with either in the winter and the house plants I have are not that entertaining so I jumped on the ban wagon of cultivation and now it has become a lifestyle. I love this website and the trades that take place...it is cool as hell. I am trying to build a decent stock of prints and trade or purchase what I can from who ever is interested. I won a contest a while back for ATL #7 I plan on getting them going here in the next few weeks. Have you grown any of these?
I am off now to search for an agar recipe...I am still intimidated on creating it from scratch but that is the only way one learns. myc
If you would like I could post more of my non active pics. myc
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Professor Frink
Mycomaniac


Registered: 11/02/08
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Re: transferring agar cultures [Re: Mikeallojee]
#12351445 - 04/07/10 08:18 PM (14 years, 1 month ago) |
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What the hell is that? That's a cube right? The gray color is confusing the hell outta me, never seen one like that....
You need to clone that and for sure get a damn spore print
-------------------- Culture trade list Wanted Culture list: Panellus stipticus Yellow Morel
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PrimalSoup
hyperspatial illuminations



Registered: 11/17/09
Posts: 13,568
Loc: PNW
Last seen: 1 year, 8 months
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Re: transferring agar cultures [Re: mycoelf]
#12351610 - 04/07/10 08:52 PM (14 years, 1 month ago) |
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Quote:
That is their purpose, to speed our evolution so that we can become tech monkeys and carry life as a whole to other planets in space!!
Including spores in space - the master plan.  
@Mikeallojee: That white fruit is a nice sport. Be interesting to see how the cloning turns out!
Peace -PS
--------------------
if you stand too close to the machine it'll start to eat youPrimal's simple tested teks and projects: Wheat Prep 2.0 Acidic Tea Tek Potency Project!
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Mikeallojee
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Registered: 10/02/09
Posts: 897
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Re: transferring agar cultures [Re: PrimalSoup]
#12352141 - 04/07/10 10:29 PM (14 years, 1 month ago) |
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http:// http:// http://
Here are three samples taken off the grey mushroom. Notice that one looks kinda normal with a little contamination. The other two look odd to me. They started out with standy looking hairs from the sample. All clone pieces were taken from various spots of the stipe. Low, mid and high. Ideas of what may be happening??? myc
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mycoelf
Agent Of Chaos



Registered: 06/26/09
Posts: 555
Loc: hyperspace
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Re: transferring agar cultures [Re: Mikeallojee]
#12354212 - 04/08/10 09:58 AM (14 years, 1 month ago) |
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3127110 looks fab, there is some kind of bacterial contam going on in the lower edge of the plate, contams on the edge means your fingers got too close to the edge, something hopped the wall. This type of contam is easy to clean, a couple of drops of peroxide via syringe will react on the contam direct, your best fans is lower left, I would sample at 3 or 4 o'clock, invert the inoculum so that the myc makes contact with a peroxidated agar and your culture should be clean. Just based on looks and intuition I would pursue this substrain on a small scale, just to see what will come of the mutant mushroom. I also noticed that there is a real nice blueing going on where the clone flesh was inoculated-cool, this means that this particular strain is going well and getting everything it needs for active compound production.
I have grown PC parallel with yeast culture and got similar flat looking distorted color, I really do wonder what is going on with the one shroom, where the rest is normal looking.
The mushies in the tray look to be cub's ???? I thought that you were growing cyan, Are we looking at a wood sub in these trays???
I have had success growing on wood indoors myself-- intrigued as I have always wondered if P cyan would fruit on wood in a pod setup?????
Congratulations, you are more accomplished then you give yourself credit for- you obviously have a grasp of basics- now it is just a matter of time.
I also cultivate a large variety of edible and medicinal. MY best crops are reishi and Shitake. I am glad that you have a broader interest, I started out with cubes like everyone else, found out how deep the rabbit hole really goes,
Welcome to brotherhood
Mycoelf
-------------------- Mycoelf Sterility is a process that can be likened unto infinity, which is a long walk, the closer to the end you start before beginning, the more achievable the goal of infinity becomes. Remember, cleanliness in next to goddessness
      
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Mikeallojee
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Registered: 10/02/09
Posts: 897
Loc: SW WA
Last seen: 11 years, 3 months
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Re: transferring agar cultures [Re: mycoelf]
#12354974 - 04/08/10 12:22 PM (14 years, 1 month ago) |
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Thank you for the compliment. I certainly don't feel accomplished. Yes they are cubes...I thought I would start out with something easy to grow first and from my studying cubes and oysters seemed to be the best way to start i figured that once I had some success I would branch out. I have several mushrooms grows happening all at once. I have reishi growing too. I had some kick ass antlers but I bumped the tub and the suckers just fell off...big bummer. I am growing them for friend who is Dx with terminal cancer. I dried the antlers boiled them up making a tea and gave the tea to him. I hope it helps. Since the first reishi grow which I began on alder wood dust and chip I have moved on to paper/cardboard. I noticed that the cardboard that I soaked in a coffee/gypsum mixture is taking off. Where as the ones with cardboard just soaked in water have been moving slowly.
I also have two strains of shiitake. One from FP and one 75 from noobieshroomy. The blocks I have going now are clones from a block I bought for a friend who was Dx with prostrate cancer a few years ago which is in remission. I read that shiitake help reduce the return of some cancers so I decided to buy him the block with the plan of cloning and continuing the cycle so he can always have fresh ones for his salad or what not. I think I placed too much bran in the block because all I have growing now are big blobs. RR said to pick them off which I did. I now have four petri dishes with 75 on it that need to be placed in some grain jars. Just getting around to is seems to be the biggest struggle.
Thanks for the advice on the petri dish. Do you have any idea as to why the other two dishes grew the way they did with the strandy looking mycelium and the other dish performed much better? I do plan on doing some small scale experimenting on the the white cube just for fun. The anomaly has really caught my interest.
I had no idea that placing my figures too close would cause a contamination to jump the wall. It isn't the first time this has happened I just wrote it off as, "oh well it happens." Peroxide for the contamination what great idea. I will have to do this. I kinda like watching competing fungus compete to see what will happen.
The sub in the trays is CCV with no casing layer. It occurred on the third flush.
On your signature is that a PE shroom? Did you grow it in a bag? myc
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mycoelf
Agent Of Chaos



Registered: 06/26/09
Posts: 555
Loc: hyperspace
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Re: transferring agar cultures [Re: Mikeallojee]
#12355872 - 04/08/10 02:26 PM (14 years, 1 month ago) |
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Hello,
My first strain of cultivation was PC, var golden teacher. I believe that there are some mushrooms that are so damn hardy as to allow you to make all of the mistakes in a run and still they are so forgiving/ hardy they still pay you with a moderated success. Cubies are one, oysters are another. The tag of "golden teacher" took on a new meaning as I first tuned into what was going on on the dish with them. I was growing cub's and studying my ass off, while one day I was sitting outside in to forest reading about mushrooms and Lo and behold I found a wild Reishi. I preformed a low impact tissue sample/ clone and met with perfect success this was the pinnacle of my then growing love of the myc. when I came to understand what power the Reishi had I realized that ALL mushrooms have something to offer, and the world was bigger than just growing to trip w/ my friends. I have been taking reishi for years now and wouldn't do with out it.
As for the differentiation in your clone, your guess is as good as any. For Stopharia type's I usually start with a longitudinal dissection, and examine a the interior. I am a believer in a quiet mind while in sterile arena and I wait for a chunk of fuzz to call to me for transfer. This is the intuitive process that I have referred to in the past, with cubes the long ropey myc in the core seems to be the winner. I have cloned the majority of my strains from the wild and have spent many hours pondering the subject. It may just boil down to getting lucky, I keep a 10x 60 mm plate for cloning and strain work They are so cheap, like 500 for 80$ that I pour special runs just for this purpose. If I really want a clone I do 10 plates, if it is precious I do 20, if it is just another strain of oyster I may do a few as 3. I have found that the myc that leaps off from the wild clone is as aggressive as it gets, I try to go in-vitro within 2 transfers of this clone. This is when the myc is in top genetic form and should be preserved thusly.
There is an excellent discussion on myc archetypes in GGMM, you might check it out, and while there look at the section (so dry) on hand position protocol while handling plates in sterile arena, it takes a bit of practice but once you get what he teaches there you wont commit the same mistakes over and over, train you hand how to make those motions and when habit is burned in you won't have to give it another thought. You will also save yourself some work and expense by not having to re-mediate cultures.
my sig pic is the "Mazetec" strain that I mentioned earlier in this thread, it weighed in at 155g wet and was part of an experiment fruiting on an enriched wood substrate spawned in a 5lb SAB, run flat with a vermic casing layer in an aspirated mono tub type setup. I don't know what you mean by "PE"
time to go feed the critters.
Mycoelf
-------------------- Mycoelf Sterility is a process that can be likened unto infinity, which is a long walk, the closer to the end you start before beginning, the more achievable the goal of infinity becomes. Remember, cleanliness in next to goddessness
      
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Mikeallojee
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Registered: 10/02/09
Posts: 897
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Re: transferring agar cultures [Re: mycoelf]
#12358298 - 04/08/10 09:27 PM (14 years, 1 month ago) |
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"try to go in-vitro within 2 transfers of this clone" What is meant by invitro?
PE=penis envy. I saw another post where the person had grown them in a bag and they were as big as the one you have there. Its obvious that I still have a lot of learning to do as it goes for identification. I will go to the GGMM and look for the hands technique and strain archetype. I had never thought of taking 20 cultures from one mushroom, that's one sleeve that I am using. But $80 for 500 sounds like a good deal. Where do you get your dishes from?
I just poured 30 agar dishes today, I plan on expanding the white clone in about five days. As well as the shiitake 75 I have. I want to wait to make sure there is no contamination before I do though.
Reishi? what do you use for your sub? Do you have a specific type of agar you use specifically for reishi? You also said that you found a wild specimen of reishi that would be a great find. I talked to the wife about spending our summer vacation out on the Washington peninsula. I was thinking that I might get lucky and find some wild samples out there. I also go hiking alot in the cascades but until about three months ago I didn't know what reishi was. Reading a thread on a guy who made a reishi monotub that kicked ass. I want to try one of those as well but am afraid that it will fail miserably. Since I have other people counting on me to provide, I want to make sure I am successful so I will continue with the wood blocks and paper/cardboard. I would love some advice on reishi as well. Tips are always appreciated. myc
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badman


Registered: 06/14/06
Posts: 4,039
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Re: transferring agar cultures [Re: Mikeallojee]
#12358437 - 04/08/10 09:50 PM (14 years, 1 month ago) |
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In vitro means behind glass, with shrooms...I like these pics
   
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mycoelf
Agent Of Chaos



Registered: 06/26/09
Posts: 555
Loc: hyperspace
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Re: transferring agar cultures [Re: badman]
#12363944 - 04/09/10 07:37 PM (14 years, 1 month ago) |
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Hello,
As badman astutely pointed out, the literal definition, I was referring to storage slants. These are test tubes that are autoclaved then "slanted' to cool causing great surface area for colonization. I have been getting the 20x 100 that FP offers, I am looking further into lab supply when I next order a collection. They have screw on lids and are easier to handle while inoculating. Once grown out they are kept in the fridge. You can then revive restart strains from these masters when needed. I would think the standard is 10 transfers from origins would be considered prime, but we all know that a good Myc will go for many more transfers than that before collapse.
I have always referred to stock cultures as IN-vitro. Perhaps this is wrong, I scanned GGMM this morning and did not see the term associated with the appropriate section. I suppose I shall have to adjust my language to fit the definition closer.
http://www.tritechresearch.com/T3308.html
These are the plates that I was referring to. They seem to have dropped in price since last year when I bought last. They also have a vented version, but I would say since you are working at home you may prefer the ones that I am accustomed to.
where Reishi are concerned I have two strains that I cloned from the wild. I have found them very easy to clone once you adapt to the polypore physiology, and learn to clone at the base of the shelf anywhere above the pores in cross section. I really like polypores, they are SO aggressive in culture, I don't make any special provisions, they are fed what ever agar that I have made for my other work, I can innoc a 100 mm plate and get a full colony in 5-7 days. Polypores then to thicken as they mature on a plate and can be difficult to cut with a razor sharp scalpel. I have never expanded them to senescence, as it is bad practice, I suspect that they would go even further than your standard cube before failing.
As for substrate they are as easy to please as on a plate. I get sawdust from a stone industry palate maker, mixed hard wood, oak and poplar, even with some assorted pine shavings and they eat it all. Stametes states that augmenting sawdust with grain at greater than 10% can actually reduce yield. I like to use rice or WBS or red and white millet for grain masters, and I usually match the enrichment of the sawdust with the same grain as so they are prepared enzymaticly for colonization of the bag. They grow so fast when you set them up right that thermo-genesis is the biggest danger.
Out where you are there is the "Oregoneese" variety that should be easy enough to find for a rare mushroom, don't bother looking until summer solstice as their fruiting time is from about June to September. They like the warmth of those months.
My cloning technique is similar to MR Stametes as it was inspired by him. I have a 5/16 stainless steel tube that I sharpened in a lathe. I keep it in my "field collection kit" that is a converted camera case. I alcohol the tube while holding the other end with tweezers, flame it with my bic, then holding the un-sharp end with gloved hands I insert the tube for the underside with a twisting motion cutting a core as I go. when I sense that I am well above the pores I change the angle of the tube thereby,severing the end of the core, once I am sure that I have liberated the core, I withdraw the instrument and quickly drop into a baggie, All this with gloves and alcohol on the hands.
Upon returning to the lab I prepare sterile arena, and use a sterile solid round stainless steel that fits in the handle end of the tube to push it out onto a prepared stainless dissection tray. I usually drop some peroxide onto the specimen and wait for the reaction to stop at @15 min. Then U use a pick to secure one end and slice away the pores and move them down stream from the remaining clone. The remainder minus the field end wich is also removed and discarded is sliced into thin sections like cookie dough, speared with a scalpel and neatly each "cookie" section is placed flat into its own dish.
Cloning wild specimen's is always a crap shoot. That is why I like to use the 60mm plates, they are cheap and I pour no less than 80 at a time. Sometimes I get 7/10 clean then pick the best looking plates for further work. sometimes I get 3/ 10 clean and it is good to have a clean plate to start with then to try to separate using subculture technique.
Where fruiting Reishi is concerned, once they colonize and start to form "snakeheads" I strip or roll the bag down and place them out side. They grow kind of dry so You don't worry about watering them. They form a "skin" around the sawdust block and do quite well retaining moisture. I have run them both flat and as a block and the fruits on the flat seem to be more numerous but smaller. I have seen no evidence that a casing layer in any way helps. When I have run side by side comparison, I would say that casing a culture of Reishi is actually detrimental

This is the first one that I found and cloned in-situ as to allow the mother mushie continue to sporulate. If not "Rare" they are definitely not common. I feel as if I should let those spores do their thing in the wild. I have attempted to collect Reishi spores and failed in the past.

This is the same mushroom showing two wounds where I sampled tissue. I monitored this mushroom until the end of the year and the sampling appeared to have no negative effects.

These are resulting outdoor fruiting colonies from the above mother fungi.

This is a Reishi that I found last fall just before I closed my last lab and moved. It was found in an old growth forest on the remains of a huge tree that was more than likely to have been virgin timber. I made several successful clones and many successful culture slants. I have yet to give this one a trial. The mushroom in this pic weighed 2 oz dried, and was 6 inches across. I have high hopes for this strain.
May the myc be with you all, Mycoelf
-------------------- Mycoelf Sterility is a process that can be likened unto infinity, which is a long walk, the closer to the end you start before beginning, the more achievable the goal of infinity becomes. Remember, cleanliness in next to goddessness
      
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Mikeallojee
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Re: transferring agar cultures [Re: mycoelf]
#12365320 - 04/10/10 12:01 AM (14 years, 1 month ago) |
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Those are beutiful reishi. I hope the paper works, I was kinda sketchy about using it but part of this hobby is expereimentation plus I have seen other people on here being successful. Thank you for the wild culture extraction technique. I can't imagine how bad I might have mangled the first wild reishi I found with out your instructions. The tube idea is good. I need to learn more about poly pores there seeme to be alot around the area. I think I will buy some of those petri dishes 500 for 49 bucks is a good deal. mcy
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