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Invisiblehamloaf
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PF tek and petri dishes Answers (update) 3-23
    #12199553 - 03/14/10 11:44 AM (11 years, 7 months ago)

PF tek: Would it be alright or do you forsee any problems with using "medium" vermiculite for pf cakes?

Petri Dishes: Does any body know of or is it even possible to sterilize used plastic petri dishes for re-use? Can't afford to buy more at the moment and if I could. I would just get glass petri dishes. Used plastic perti dishes is what I have at the moment and I am ready to go.


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Edited by LiquidMyce (03/23/10 04:34 PM)


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Offlinebuggers
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Re: PF tek and petri dishes. Q/A [Re: hamloaf]
    #12199578 - 03/14/10 11:51 AM (11 years, 7 months ago)

Your vermiculite will most likely do just fine.

As for your dishes. Don't try to PC them... you'll have molten dishes. I've heard of people soaking them in alcohol.  Not sure if just boiling them would melt them or not but you could try it... just make sure they don't touch the sides or bottom of the pot.

I found a killer deal on some nalgene ones on ebay.  They're autoclavable and shatter proof.


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InvisibleDoc_T
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Re: PF tek and petri dishes. Q/A [Re: buggers]
    #12199591 - 03/14/10 11:54 AM (11 years, 7 months ago)

Quote:

buggers said:  They're autoclavable and shatter proof.




Fishing place had little PP5 boxes with separate lids on each section.


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Re: PF tek and petri dishes. Q/A [Re: Doc_T]
    #12199609 - 03/14/10 11:59 AM (11 years, 7 months ago)

Quote:

Doc_T said:
Quote:



Fishing place had little PP5 boxes with separate lids on each section.




Are you referring to those little things that sinkers come in?


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InvisibleDoc_T
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Re: PF tek and petri dishes. Q/A [Re: buggers]
    #12199611 - 03/14/10 12:01 PM (11 years, 7 months ago)

Yep. All different styles, and not too expensive.

They aren't very clear, if that's a factor.


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Offlinedeucedbi9
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Re: PF tek and petri dishes. Q/A [Re: hamloaf]
    #12199779 - 03/14/10 12:49 PM (11 years, 7 months ago)

Quote:

hamloaf said:
PF tek: Would it be alright or do you forsee any problems with using "medium" vermiculite for pf cakes?

Petri Dishes: Does any body know of or is it even possible to sterilize used plastic petri dishes for re-use? Can't afford to buy more at the moment and if I could. I would just get glass petri dishes. Used plastic perti dishes is what I have at the moment and I am ready to go.




my understanding,never done them personally,is that the larger size vermiculite gives a more airy substrate=good.
maybe sieving it through something like a spaghetti strainer would at least get rid of the more powdery portion.

as for reusing petri dishes,the ones i have will begin to melt before you've even put the weight on the pc.

for those of us that,at the moment,just want to obtain clean samples from uncertain prints...
rather than making numerous isolates of various species at a time,in which case you would likely buy by the crate-load and use a sleeve at a time.
...the occasional incovienience of carefully cleansing a couple of plates is of less importance. if you can't afford more,then you may as well try.i certainly will.
good luck


Edited by deucedbi9 (03/14/10 12:57 PM)


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Re: PF tek and petri dishes. Q/A [Re: deucedbi9]
    #12199883 - 03/14/10 01:21 PM (11 years, 7 months ago)

Verm on the finer side works just as good as medium  verm in my experience.
As long it's no straight powder, you should be OK.

It tends to be a little more dense, but if you pack the jars softly, this shouldn't lead to any problems.

Since there's plenty of different kinds of plastic petri dishes, the question is hard to answer.

You probably just have to ask weather they are suited to be heated up to the required temps, or not.


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Offlinedeucedbi9
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Re: PF tek and petri dishes. Q/A [Re: Fahkface]
    #12200358 - 03/14/10 03:14 PM (11 years, 7 months ago)

Quote:

Fahkface said:
Verm on the finer side works just as good as medium  verm in my experience.
As long it's no straight powder, you should be OK.




indeed.again,i have no personal experience of verm as far as cakes are concerned.
i was reminded of a post a by a major advocate of cakes 'fahtster' in this thread>
http://www.shroomery.org/forums/showflat.php/Number/6753704#6753704

just extrapolating that the less of the powdery portion(in the substrate) the better.ie sieving it.

he does though still advise fine verm for the barrier.
so,OP. if all you have is medium,perhaps a quick blast in a blender will be sufficient to turn your "medium" into "fine".
good luck


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Re: PF tek and petri dishes. Q/A [Re: deucedbi9]
    #12200460 - 03/14/10 03:35 PM (11 years, 7 months ago)

I have tried both and have mixed feelings. My super fine bottom bag verm is great for dunk n roll stage as it adheres really well. As far as using fine for in the cake itself, I feel it allows me to get more mix in the jar, as by volume it should have more weight. I also wonder about its filtering properties in the dry layer. Shouldn't the smaller gaps between pieces allow less airflow and increase the chance the enemy spore does not drift through? Probably more prone to becoming moist however?

But the bigger stuff is tried and true, and a bit more forgiving as far as water content and over packing. I would recommend sifting out the smaller bits and saving them for the roll stage. Don't forget to bake it first for extra protection.


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Re: PF tek and petri dishes. Q/A [Re: SomeNewGuy]
    #12200468 - 03/14/10 03:38 PM (11 years, 7 months ago)

Quote:

I would recommend sifting out the smaller bits and saving them for the roll stage. Don't forget to bake it first for extra protection.




i have been saying for a while now that finer verm is best used for the dry roll. Dont look though the whole bag and shift for the smaller pieces of verm. Just run some verm though a coffee grinder a few times and make a foil pouch and keep it in there, Then al lyou have to do is bake the pouch at 350 for 20-30 minutes and use it when its cooled down


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InvisibleSomeNewGuy
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Re: PF tek and petri dishes. Q/A [Re: Shea25]
    #12200578 - 03/14/10 03:57 PM (11 years, 7 months ago)

^ Genius to save it in a pouch!

Sorry to go off topic hamloaf but who is your avatar? That guy looks crazy as fu**!


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Edited by SomeNewGuy (03/14/10 03:58 PM)


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Invisiblehamloaf
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Re: PF tek and petri dishes. Q/A [Re: SomeNewGuy]
    #12200640 - 03/14/10 04:13 PM (11 years, 7 months ago)

HAHA....That was the last picture they took of before I got out. I had just gotten out of Seg.


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Invisiblehamloaf
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Re: PF tek and petri dishes. Q/A [Re: hamloaf]
    #12213536 - 03/16/10 05:40 PM (11 years, 7 months ago)

:calledajoke: :laugh2:

  I gave the the medium coarse verm a whirl. We shall see how it goes:popcorn:

  As far as those plastic petri dishes. I took a hammer to them.

  I mixed a batch of MEA. :stirthepot:  Took 4 ounce mason jars and threw them all in the PC for 20 minutes at 15 psi. Let it cool enough that I can handle the jars and more importantly. The hot agar mix. Without burning myself through the gloves. Got out my glove box. Then poured my agar into these 4 ounce jars at about a quater of an inch of agar high.

  Now I'm like forget plastic petri dishes for life.

  I'm like forget petri dishes all together.

  At least til I come up with the  :money: for the construction of my own flow hood.

  Still. What is so great about petri dishes anyway. These little 4 ounce jars really impressed me. You get the quality of glass both affordable, readily available, and easy to handle.

  No polyfill. No pain in the neck saran wrap. No seams! Just jars. With lids. That screw on and off.

  You can even put self healing inoculation points on these lids and not even have to open them until it's time to transfer.

  When your done with them. Just wash them out with bleachy water. Then soap and water. Throw them in your PC at 15 psi for 20 minutes to use again.

  Brilliant.  :super:


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Re: PF tek and petri dishes. Q/A [Re: hamloaf]
    #12214665 - 03/16/10 08:38 PM (11 years, 7 months ago)

How's visibility in the jars? Do you get a good view of sectoring?


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OfflineDamion5050
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Re: PF tek and petri dishes. Q/A [Re: Doc_T]
    #12214678 - 03/16/10 08:40 PM (11 years, 7 months ago)

Question for you.  Did you PC the agar ?  I don't see that in your write up.. I hope u did.  Cause you should have heated up your agar mix then PC'ed that for 20-30 minutes as well as your jars.


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Invisiblehamloaf
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Re: PF tek and petri dishes. Q/A [Re: Damion5050]
    #12215384 - 03/16/10 10:36 PM (11 years, 7 months ago)

Thank you for the interest in my thread.
Quote:

Doc_T said:
How's visibility in the jars? Do you get a good view of sectoring?




  Visibility in the jars is decent. It would be better in deeper jars. Depending on the size of your hands. Then wider would be keen also. For cutting chunks of the live culture out.

  I figured when the mycelium reaches the sides of the jars. I will get out my glove box and do the best I can to view the sectoring through my glove box.

Quote:

Damion5050 said:
Question for you.  Did you PC the agar ?  I don't see that in your write up.. I hope u did.  Cause you should have heated up your agar mix then PC'ed that for 20-30 minutes as well as your jars.



  Damion, Damion, Damion. Did i PC the agar.

  I PC'ed the agar right along with the 4 ounce jars in the PC for 20 minutes at 15 psi.

  Then I poured the agar into the 4 ounce jars when it had cooled off enough to handle yet nowhere near solidification.


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OfflineDamion5050
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Re: PF tek and petri dishes. Q/A [Re: hamloaf]
    #12215422 - 03/16/10 10:43 PM (11 years, 7 months ago)

Hey just checking, you never know now a days.


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Invisiblehamloaf
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Re: PF tek and petri dishes. Q/A [Re: Damion5050]
    #12215575 - 03/16/10 11:12 PM (11 years, 7 months ago)

Hey. Forget about it. I was just kidding around. Thank you for checking to see if I PC'ed my agar mix. That's good looking out.

So. You tell me. What do you think? Pretty stupid idea. Huh.


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Re: PF tek and petri dishes. Q/A [Re: hamloaf]
    #12215806 - 03/16/10 11:45 PM (11 years, 7 months ago)

See I find the petri dishes are simply easy to stack heaps in a small space. 

The jars work fine for 4-5 'plates' but when I clone wild shrooms or use wild prints I will take a good 10-20 samples for each mushroom. 2 stacks of 10 takes up the same room as 2 pint jars.  It just lets you be a bit more lazy and throw out most of the first-round samples and select a cleaner one right from the start.

If you just doing 1-2 lots from clean spores the jar is great as I use an innoc point and can easy inject sterile water shake the crap out of it and draw it back out again and a quick and easy MS-LC :laugh:

Best is (funds permitting) getting bulk dishes I got a carton of 600 80mm dishes for $50 and that lasts a while...  Same with scalpel etc as well etc 

I'm a full-time engineering student so my wallet has its own tumble-weed!  But seriously, buying bulk every 6 months really shaves down costs. Just split between a few people if you can as well


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Re: PF tek and petri dishes. Q/A [Re: SN122A]
    #12215960 - 03/17/10 12:14 AM (11 years, 7 months ago)

Na I have seen people use jars as petri dishes.  It would be easier if someone came up with a way to make a clear lid for the jars so you can see the actual culture growing.

U can easily turn it into an LC once the agar is colonized just inject 100mL of sterile water and boom instant LC


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Invisiblehamloaf
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Re: PF tek and petri dishes. Q/A [Re: hamloaf]
    #12255758 - 03/23/10 04:41 PM (11 years, 6 months ago)

Quote:

hamloaf said:
:calledajoke: :laugh2:

  I gave the the medium coarse verm a whirl. We shall see how it goes:popcorn:

  As far as those plastic petri dishes. I took a hammer to them.

  I mixed a batch of MEA. :stirthepot:  Took 4 ounce mason jars and threw them all in the PC for 20 minutes at 15 psi. Let it cool enough that I can handle the jars and more importantly. The hot agar mix. Without burning myself through the gloves. Got out my glove box. Then poured my agar into these 4 ounce jars at about a quater of an inch of agar high.

  Now I'm like forget plastic petri dishes for life.

  I'm like forget petri dishes all together.

  At least til I come up with the  :money: for the construction of my own flow hood.

  Still. What is so great about petri dishes anyway. These little 4 ounce jars really impressed me. You get the quality of glass both affordable, readily available, and easy to handle.

  No polyfill. No pain in the neck saran wrap. No seams! Just jars. With lids. That screw on and off.

  You can even put self healing inoculation points on these lids and not even have to open them until it's time to transfer.

  When your done with them. Just wash them out with bleachy water. Then soap and water. Throw them in your PC at 15 psi for 20 minutes to use again.

  Brilliant.  :super:



PF tek is kicking ass with medium coarse vermiculite.

  Those four ounce jars are not the best for watching your sectoring form:shrug:
  I still like the jars in the place of petri dishes for now. I think for my aown specifications I will go with wide mouth pint sized jars. I think it will be alot easier to examine the sectoring in them and still have the convience of a jar. We will see.


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