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Amazon Shop for: ½ Pint Jars, Agar, Petri Dish, Potato Dextrose, Rye Grain

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Invisibledog
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Registered: 06/20/02
Posts: 2,790
Loc: Route 66
agar problems
    #1219897 - 01/15/03 05:52 AM (13 years, 10 months ago)

I've recently starting experimenting with agar and I am having some major contamination issues. Do you folks who are experienced with agar find that agar contaminates more readily than other growth mediums?

For instance, if I exposed some prepared grain and an equal surface area of agar to the open air, are the chances of the agar contaminating greater than that of the grain?

I'm wondering if my glovebox, which works fine for working with grain transfers etc., is insuffient for agar.

:confused: 


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Offlinegrowmore
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Re: agar problems [Re: dog]
    #1220278 - 01/15/03 08:30 AM (13 years, 10 months ago)

Dog agar does contam easy cause it is rich in nuts . your glove box should work just fine . Are u useing petredishes or something els, maybe you are serializing it wrong . explain how u prepar it .


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Invisibledog
straw dog

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Re: agar problems [Re: growmore]
    #1221231 - 01/15/03 01:06 PM (13 years, 10 months ago)

I used the standard PDA recipe in the FAQ, PC'd at 15 PSI for 50 minutes. After it cooled I poured it into petri dishes in my glovebox and then inoculated each with three drops from a syringe after it had set.

The same syringe was used to inoculate a couple of rye jars 1/2 hour later. The jars grew the good stuff, the dishes contamed.


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InvisiblePinhead
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Re: agar problems [Re: dog]
    #1221813 - 01/15/03 05:23 PM (13 years, 10 months ago)

Mine always contaminates! But it might have something to do with my house being filthy! :blush:


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Invisibleblahblahblah
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Registered: 10/25/01
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Re: agar problems [Re: dog]
    #1222098 - 01/15/03 07:57 PM (13 years, 10 months ago)

It sounds like you may have pressure cooked it to long, I think that after 30 minutes or so the sugars of the dextros carmelize. I think this is true for all agar formulas. I am pretty sure that 30 minutes was the maximum you should cook them but not 100%


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Offlinezeronio
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Re: agar problems [Re: dog]
    #1222320 - 01/15/03 11:26 PM (13 years, 10 months ago)

Agar contains a lot of nutrients and contaminates easily.
Try to use peroxide (H2O2) next time. It works great!


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OfflineMAIA
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Re: agar problems [Re: dog]
    #1223418 - 01/16/03 11:28 AM (13 years, 10 months ago)

How long do you wait for inoculation after pouring the agar in the petri dishes ?

MAIA


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Invisibledog
straw dog

Registered: 06/20/02
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Loc: Route 66
Re: agar problems [Re: MAIA]
    #1223426 - 01/16/03 11:33 AM (13 years, 10 months ago)

I do not recall exactly but it was at least 1/2 hour. The agar had set so I assumed it was cool enough.

Thanks for the suggestions. It's time for my next attempt!  :smile: 


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Fascism (fash'izem) n. A governmental system marked by a centralized dictatorship, stringent socioeconomic controls, and often belligerent nationalism. see also: the Bush Administration.


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OfflineMAIA
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Re: agar problems [Re: dog]
    #1223436 - 01/16/03 11:37 AM (13 years, 10 months ago)

If it was set then it was cool enough. Watch out that step, don't be in hurry.

MAIA


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Use, do not abuse; neither abstinence nor excess ever renders man happy.
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Amazon Shop for: ½ Pint Jars, Agar, Petri Dish, Potato Dextrose, Rye Grain

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