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Amazon Shop for: ½ Pint Jars, Autoclave, Brown Rice Flour, Impulse Sealer, Rye Grain, Vermiculite

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Anonymous

BAGS
    #1217928 - 01/14/03 01:44 PM (14 years, 3 months ago)

My buddy has had terrible luck trying to colonize in those spawn bags. Lots of contams. Can anyone give me some advice or procedural information so I can help him out?


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Offlinegirlskater
***meow***

Registered: 12/18/01
Posts: 260
Loc: Here
Last seen: 13 years, 8 months
Re: BAGS [Re: ]
    #1217944 - 01/14/03 01:48 PM (14 years, 3 months ago)

mind giving some more info about the procedures?


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InvisibleEthnoJimmy
Voyage thegalaxy

Registered: 12/02/02
Posts: 244
Re: BAGS [Re: girlskater]
    #1217957 - 01/14/03 01:51 PM (14 years, 3 months ago)

I've never tried bags, so I have no idea..and you didn't give any detail.
But if you want some of the best advice you can get in this hobby:
http://www.fanaticus.com/


--------------------
Don't walk behind me, I will not lead?
Don't walk in front of me, I will not follow?
Just walk beside me and be a friend.



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Anonymous

Re: BAGS [Re: girlskater]
    #1218079 - 01/14/03 02:37 PM (14 years, 3 months ago)

I just mean using a spawn bag (like those available at The Spore Works) and sealing with an impulse sealer to colonize suubstrate instead of using jars. No luck so far....


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OfflineGunther
Stranger
Registered: 04/29/02
Posts: 17
Last seen: 14 years, 20 days
Re: BAGS [Re: ]
    #1218269 - 01/14/03 04:13 PM (14 years, 3 months ago)

first you must kill all bacteria in the bag and your substrate. add your substrate into the bag. Close with a heavy duty clip or impulse sealer. Put in a pot with some water and steam boil for an hour, 2, if you want to be sure. after this place your bag in a cool draft free area i would wait probably at least 4 hours. Then take your syringe which hopefully you purchased cause it sound like you are not that clean (sterile), and rub down the injection site with isopropyl alcohol, flame sterilize your needle and rub it down with the alcohol as well. Air dry both, should happen in seconds, and then inject the solution. 2cc's for a small bag id say 4 for a medium or big one. more spots = faster colonization. Making your own substrate is tricky especially for newbies. Just remember isopropyl alcohol, lysol and oil lamps for flame sterilization are your best friends at this point. Use in excess.


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Anonymous

Re: BAGS [Re: Gunther]
    #1276606 - 02/04/03 06:23 AM (14 years, 2 months ago)

How long should they be pressure cooked for? My buddy was worried about pressure cooking them too long because the air in the bags might expand too much and pop the bags. Also, the substrate won't dry out from the temperature in this scenario?


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OfflineSillycybe
Sillycybe

Registered: 12/12/02
Posts: 75
Last seen: 11 years, 7 months
Re: BAGS [Re: ]
    #1276821 - 02/04/03 07:30 AM (14 years, 2 months ago)

Does he work in front of a filter hood?


--------------------
"Some say we'll see armagedon soon...I certainly hope we will, I sure could use a vacation."


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OfflineSillycybe
Sillycybe

Registered: 12/12/02
Posts: 75
Last seen: 11 years, 7 months
Re: BAGS [Re: ]
    #1276826 - 02/04/03 07:31 AM (14 years, 2 months ago)

If the spawn bag has a filter or air transfer port on it, you should have no prob. with bursting bags.


--------------------
"Some say we'll see armagedon soon...I certainly hope we will, I sure could use a vacation."


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Anonymous

Re: BAGS [Re: Sillycybe]
    #1276935 - 02/04/03 08:00 AM (14 years, 2 months ago)

No filter hood. What do you think about PC time?


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Anonymous

Re: BAGS [Re: ]
    #1277022 - 02/04/03 08:29 AM (14 years, 2 months ago)

I pc millet at an hour and a half


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Offlinemoldmaster
mold fighter forlife
Registered: 01/24/03
Posts: 160
Loc: contamville, Trich
Last seen: 13 years, 9 months
Re: BAGS [Re: ]
    #1277127 - 02/04/03 08:55 AM (14 years, 2 months ago)

It really sounds like you should go to sporeworks and print out the rye tek. It is under lab notes at their website. To date, I have done over 400 of those large autoclave bags and if you go by the instructions you will not have problems. It sounds like you are not letting the bacteria germ. before you attempt to sterlize. The rye needs to soak in water before you PC. Usually, you can get by with one hour of PC, but sounds to me like you might want to go 1.5-2hours.


--------------------
Wham bam kick yo ass contam. Mold doesn't even dare to enter my shit because I have the remedy


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Anonymous

Re: BAGS [Re: moldmaster]
    #1278080 - 02/04/03 01:55 PM (14 years, 2 months ago)

My buddy is using the Fanaticus Max Fruiting formula which is vermiculite, brown rice powder, and water. Is that OK in a spawn bag?


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Amazon Shop for: ½ Pint Jars, Autoclave, Brown Rice Flour, Impulse Sealer, Rye Grain, Vermiculite

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